FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT21.5384615384615385No Hit
GTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGAT10.7692307692307693No Hit
CATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGT10.7692307692307693No Hit
TATGTATATTATGCATCCATTTTTTGTAAAGCCCTTGAGTTCAAAAATGA10.7692307692307693No Hit
CCCTTGGCCTCGATATCGATACAGCCACGCTTGTTGGGAAACAAATCGTG10.7692307692307693No Hit
CTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTAC10.7692307692307693No Hit
GGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGC10.7692307692307693No Hit
CACAACTCATGGAGTCACACTCCGCGATTGTACACGGAGAACTTACTTGA10.7692307692307693No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA10.7692307692307693No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA10.7692307692307693No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGC10.7692307692307693No Hit
GCCAAGGAGGTGGCACTAATCTCGGCACCGGGTGCACGTGCCCTTGGCAT10.7692307692307693No Hit
GTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGAAGGGTA10.7692307692307693No Hit
CATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTA10.7692307692307693No Hit
GTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCAT10.7692307692307693No Hit
GCTCTTTCATTTGCGTCCATGTTTTTAATAATGTAGTAACCATTTGTGGA10.7692307692307693No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG10.7692307692307693No Hit
ATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGA10.7692307692307693No Hit
AATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCC10.7692307692307693No Hit
GCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGC10.7692307692307693No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA10.7692307692307693No Hit
CCTGATCGTGTGCAGAGATGCCATTGACGGGATCGACAACAGCTGTGCCT10.7692307692307693No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC10.7692307692307693No Hit
TTGGAGGACACATGATCAGAAGCATGTTGGGGTTTTCGCTTGTGGAAGCA10.7692307692307693No Hit
CTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAG10.7692307692307693No Hit
AAACTCTGGTAAATCCTTGTTGATTCCAGCTTTACCCCAGCTATTTCTTC10.7692307692307693No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA10.7692307692307693No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCCG10.7692307692307693No Hit
TGGTAAGAGGATAGGTAGTGATTGCAAGCGGAGGGCGTTCCCCTAGAGGG10.7692307692307693No Hit
GAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGA10.7692307692307693No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA10.7692307692307693No Hit
CTTCTTGGTAATGTTTACTATTACTATCATATTTCTTAGAAACTGTGTAG10.7692307692307693No Hit
CCATTTCCTAAACATAAGACATAATAACACTCCATCAGTAGTTATTTTTT10.7692307692307693No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG10.7692307692307693No Hit
CTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGGA10.7692307692307693No Hit
CTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGG10.7692307692307693No Hit
GGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAGCCTTTG10.7692307692307693No Hit
ACCATGGACTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAG10.7692307692307693No Hit
GAATGTCTCTGACCGTTAACTTACTAGAAGCTCTGCATCCAGCTGTCCGT10.7692307692307693No Hit
AGAGCTGACTTCCTCAGGCTCCTCGGGCAATTCCCCGGAGGCCTTGCCTA10.7692307692307693No Hit
GGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGG10.7692307692307693No Hit
TAAGTACTCTTTGCCATTTAAGGACCTATGAATCCAACTGAGGAGTAGAT10.7692307692307693No Hit
GGGTCAGTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATC10.7692307692307693No Hit
GTTCCTCCTGATGCTTGCGATATGTTCAATCGTGGCAACATCTTCTCCTA10.7692307692307693No Hit
GCCAGGGACATCCTCATGGGATTTCTTTTCTTTTCTTTTCCTTTTTTTTT10.7692307692307693No Hit
CAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAA10.7692307692307693No Hit
CTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAAT10.7692307692307693No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA10.7692307692307693No Hit
GTACATCATCTCCTTGTTGCGCCGTGGCCCCCCGAAGGGGATGAAGGGCA10.7692307692307693No Hit
CTATTAGACAAGAACTGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.7692307692307693No Hit
GTTCAAACGTGGAATCAATGACCGAAATTTCTGGAGGGGTGAAAATGGAC10.7692307692307693No Hit
AATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATT10.7692307692307693No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.7692307692307693No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGA10.7692307692307693No Hit
TGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTT10.7692307692307693No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG10.7692307692307693No Hit
CCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCAC10.7692307692307693No Hit
AGATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGTGATTCCGAGAG10.7692307692307693No Hit
TGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCA10.7692307692307693No Hit
CTGGGAGGCCCGGGAGCGGGGAGGCGGCGGCTGGGGGATTAGGGCCGGGA10.7692307692307693No Hit
ATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTC10.7692307692307693No Hit
GCGTGGGTCCGGGGGGTCGCGGGGGTGGGGGGGGTGGGGGGGGTGGGGTG10.7692307692307693No Hit
GTCTAGCCGAGGTCGAAACGTGCGTTCTTTCTATCGTCCCGTCCGGCCCC10.7692307692307693No Hit
TGGAGCAAGGGGGGGATCTAGGCCCGATGTTTCTGGAGGGGGGAATATGG10.7692307692307693No Hit
TCTAGATTGTGCTCACCCAGTGGTAGATGCAAATGGTCAGAGCGCTATTA10.7692307692307693No Hit
GAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACG10.7692307692307693No Hit
CCCCACTTCTGGAAAAGCTCGATTGCATCTTTTTGGAAATGCCTCAAGAG10.7692307692307693No Hit
GGCCTGATCTTCTGGCTTTGCACGTTCCTTCATTCTTAGTAGTGCTGGTC10.7692307692307693No Hit
CGGTCAGCACTCATTCTGAGGGGATCAGTTGCACATAAATCCTGCCTGCC10.7692307692307693No Hit
GTATTGGGGCGCCCTGCAGGGGGAGGCTTGGGGTGTTCGATAGTTGCGCC10.7692307692307693No Hit
AGAAAGGAGAGATATAATGTAGCAGTCACGAGCTAGCGAGATACGCACTA10.7692307692307693No Hit
GTTAGGTCCTGCACACTTTCCCAATAGAGCCTTCCGCCACTCCCCTGGGT10.7692307692307693No Hit
CAGTCAGTGTGCAAATCAGTGTTCTGGTCTGTTCTGGTTTTGCATGTAAT10.7692307692307693No Hit
GAAAATAAGAGGACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA10.7692307692307693No Hit
GCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAG10.7692307692307693No Hit
GATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATG10.7692307692307693No Hit
CCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAG10.7692307692307693No Hit
GAGTTGTTTTCTAGCCCCTGAGGGTGGTTGGTGTGTAGTTCCCACAGGCT10.7692307692307693No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA10.7692307692307693No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC10.7692307692307693No Hit
GTTTTTAACCTGAATATACATGACTCCCATTCTGCTCTACTGCGCCTCTC10.7692307692307693No Hit
AATATAGGAACATGGTCCTCGACCATTGGCCTATCGCAGTCTTAAATCGC10.7692307692307693No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA10.7692307692307693No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC10.7692307692307693No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCACTTTTCAAACCGTA10.7692307692307693No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG10.7692307692307693No Hit
ACTGTACACTGACAGACTCTCTCCTTGACCACATTTCAGCCGGCTTCCTT10.7692307692307693No Hit
GGGAGGGAGGTGGTGGGCGCCGGCGGCGGGTCGGGGGGGGCGGGGGTCTG10.7692307692307693No Hit
CCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATCGC10.7692307692307693No Hit
AACAAAATAATTATCAACATCTGAACAAAATACACTATAAAATAAAAGAT10.7692307692307693No Hit
GGACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC10.7692307692307693No Hit
CCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGG10.7692307692307693No Hit
GCTCAGTCGGTAGAGCACACAACTCCAGATTTCGGGGTCATGAGTTCAAA10.7692307692307693No Hit
ATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTC10.7692307692307693No Hit
CCAGCGACTGAGCCCCATGCACCGACTCTTGAGGGATTTCCAAAAAGCTG10.7692307692307693No Hit
GTACATAAATCTCTGTACCTCTCTTTTCTTTATAAAGGAGGAATATCTTT10.7692307692307693No Hit
GCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGA10.7692307692307693No Hit
CTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCT10.7692307692307693No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC10.7692307692307693No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC10.7692307692307693No Hit
CATTATAGGTGCCCTGCGCAGGATGTTTCTGAACCACTCGGGTTGATTGC10.7692307692307693No Hit
CCATAGAGCCTTCCTCCACTCCCCTGGGGGACTCTCCGATTGGCCATGTT10.7692307692307693No Hit
GCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGT10.7692307692307693No Hit
GTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCA10.7692307692307693No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT10.7692307692307693No Hit
GCTTCATATAGCCCCCCTTGATCGAAGGTTCCAGGTTGCAGGTTGTCGCT10.7692307692307693No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT10.7692307692307693No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT10.7692307692307693No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG10.7692307692307693No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCGCCGTGCCCAG10.7692307692307693No Hit
CTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTT10.7692307692307693No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA10.7692307692307693No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC10.7692307692307693No Hit
GATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCT10.7692307692307693No Hit
CTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGC10.7692307692307693No Hit
CTCTGCGGTCGCCTGGAGGGGGGGCGAGGAGGCGTCAGGGGGGGCGCCGT10.7692307692307693No Hit
AAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGT10.7692307692307693No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.7692307692307693No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC10.7692307692307693No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG10.7692307692307693No Hit
ATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGG10.7692307692307693No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC10.7692307692307693No Hit
GACATGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGA10.7692307692307693No Hit
TGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCT10.7692307692307693No Hit
GTGTTGTGCTGACAGGTTGGGCTCGAGGACCCAGAAGTGACCCTCGAAGA10.7692307692307693No Hit
GAGCAGATGCTTCCCTACGGGCAGATGGAAATAAACCATTAGACTTAACT10.7692307692307693No Hit
GGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTA10.7692307692307693No Hit
CTCACCCACTATCCGCGAAAAATTTAATAAACAACAAAAGCATAAGTTCA10.7692307692307693No Hit
GTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCATCAATG10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers