FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAA14.545454545454546No Hit
ACATTCCCTTATACTGGAGATACTCCATACAGCCATGGAACAGGAACAGG14.545454545454546No Hit
CCAATGGAACCTCCAAGATCAAGATGAAATGGGGCATGGAAATGAGGCGC14.545454545454546No Hit
TCAAGGAAGTAACTTAATATTGTCTCCCGAAGAAGTCAGTTAAACACAAC14.545454545454546No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAG14.545454545454546No Hit
GGTCGTCATTAAACCCTTAGACAAAAATTTATGGAAATACGAAATATTAT14.545454545454546No Hit
GTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCAC14.545454545454546No Hit
GTGCATGTGAGAGGAAGTAGTTGAACCAAGATGCATAAAGAAAAACCCAC14.545454545454546No Hit
ATCTTTAACCGATTAGAGCCCTTGATACTACTAAGGGCTTTCAATGAGGA14.545454545454546No Hit
AGCAGGTACTGATCCAAAATGGAAGCCTTTGTGCGACAATGCGTCAATCC14.545454545454546No Hit
GATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCA14.545454545454546No Hit
GTTAATCAGACAGCCATGCCATCCCCACCGAGACCAAGTGCTCTATAAAA14.545454545454546No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAAAATCTAATGAGTAAGTAATT14.545454545454546No Hit
GATTAGAGGTTTCTTAAACTTTGTTGAAACTTTATCTAGAAGAATTAGAC14.545454545454546No Hit
TTTTCAGTGTTTTGAGGTGGCCATGTAGCACTTCGTCTTATTTATTGACT14.545454545454546No Hit
GGCATCAATCCCGGCCATAGAAACCATGGCCTCCACCATACTAGAAATAC14.545454545454546No Hit
GTCATACTCCTCTGCATTGTCTACGAAGAAATAAGACCCTTCAATACTCA14.545454545454546No Hit
ATTGACCTCTGGGAAGCTGTTATAAATTTTCCTATGAGGATATTGCACAT14.545454545454546No Hit
GGCCTATCGAAGTCAGCAAGCGAAGGTCTACTATTTCAATAACACAGTAT14.545454545454546No Hit
GCTTGAGGATAAGATCATCTAACAGGTTTGGTGGGTTCACTTTCAAAAGG14.545454545454546No Hit
AATTGCAGACAATGGATTTGGTGATGCAACATTCCTTGATCGGCTCCGCC14.545454545454546No Hit
TCCAAGGCCTCCGCATCCTGATCACACGATCCCGCCATATGTTCCATAGC14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)