FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC40.3088803088803089No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.23166023166023164No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA30.23166023166023164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.23166023166023164No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.23166023166023164No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.23166023166023164No Hit
ATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACA30.23166023166023164No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA30.23166023166023164No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC20.15444015444015444No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.15444015444015444No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT20.15444015444015444No Hit
CAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGA20.15444015444015444No Hit
GTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTC20.15444015444015444No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20.15444015444015444No Hit
GGGCAAGAATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGT20.15444015444015444No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC20.15444015444015444No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG20.15444015444015444No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT20.15444015444015444No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG20.15444015444015444No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA20.15444015444015444No Hit
CTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAG20.15444015444015444No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.15444015444015444No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG20.15444015444015444No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20.15444015444015444No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.15444015444015444No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC20.15444015444015444No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA20.15444015444015444No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC20.15444015444015444No Hit
GTGGTGAACAGTATATGTAACACAACAGTGGTAGAGAAGCCTAAATTTCT20.15444015444015444No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT20.15444015444015444No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.15444015444015444No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.15444015444015444No Hit
TCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACA20.15444015444015444No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA20.15444015444015444No Hit
ATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTG20.15444015444015444No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG20.15444015444015444No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.15444015444015444No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA20.15444015444015444No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC20.15444015444015444No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA20.15444015444015444No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.15444015444015444No Hit
GGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCA20.15444015444015444No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT20.15444015444015444No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.15444015444015444No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA20.15444015444015444No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT20.15444015444015444No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC20.15444015444015444No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20.15444015444015444No Hit
GTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCA20.15444015444015444No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG20.15444015444015444No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG20.15444015444015444No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCA20.15444015444015444No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG20.15444015444015444No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA20.15444015444015444No Hit
TCTTTGGTCCTACATTTGCTTATCATTGGGCTCAGCTGAAAGTCATCCAT20.15444015444015444No Hit
GAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCT20.15444015444015444No Hit
TGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGCATGGAAA20.15444015444015444No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT20.15444015444015444No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT20.15444015444015444No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)