FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATTTATATAGGACTTCTTTTTGCTCAGGTTGATGCCCACGAACTT13.4482758620689653No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC13.4482758620689653No Hit
GTGCTGGAATAACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTG13.4482758620689653No Hit
GGCTAAAGACAAGACCAAGCGTGTCCCCTAGGACGAAGGGAATTTTAGGA13.4482758620689653No Hit
GATCGGTGGCCGCTAGCGCCTGCGTGGCGGCGTGGGGGGCTGGGCTTGGA13.4482758620689653No Hit
AATGATGCTGCTGTCAACAATGCAGTTGGGGTCCTCATCGTAGGACTGGA13.4482758620689653No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA13.4482758620689653No Hit
GAGAACTCCATACTTACAAAGTTCCCCACATCAGGATCATTTCTCAAATG13.4482758620689653No Hit
CAAGAATATAGAATTAGGGTCATATGTGCAAGACTCTTTCCCATTTCAGG13.4482758620689653No Hit
GGAATAACAAGTGCAACGCATTATTTACAGCTTTACAAACCAGGGCAACA13.4482758620689653No Hit
CTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGAGGAGTGGC13.4482758620689653No Hit
CCCCTATGGCACCGATATGGGTATCTGTAGTCTTTGAGGCACAAGTGACG13.4482758620689653No Hit
CATATAAATGATGCATTAAAATGAATGAAGACAGTCTTTGGATTGAAAGA13.4482758620689653No Hit
CCTGTAAGGTCCTGCAAACATTACCAATAGAGCATTCATCCACTCCCCTG13.4482758620689653No Hit
ATTACGAACAAAGTAAATTCTTTTATTGAAAAGATTAATACACAGTTCAC13.4482758620689653No Hit
GGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG13.4482758620689653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.4482758620689653No Hit
GTATGGGGGCTGGTCGTGGGGGTTTGTTGGGTGGTGGGGGTGTGGGGGCG13.4482758620689653No Hit
ATATTGTCTTGGTGTATTTGTTTTGTCCATGAATAAGTATCGAGACTCAC13.4482758620689653No Hit
TGAGATATCTAATTTCGCAGTACCGCACTCGAGAGATACTCACTAAGACA13.4482758620689653No Hit
GTAATGGATTCAATGAACAAAGAGTAAATAGAGATAACAACCCACTTTCA13.4482758620689653No Hit
GAGAAGAACCACGCACTCATAATTCAGTTGATGATGGCAATGATATACCC13.4482758620689653No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG13.4482758620689653No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG13.4482758620689653No Hit
TTCCGCAGGCAGCGCCGAGTGGTGCCGATGGCCGTCTGTATCAGGATGCA13.4482758620689653No Hit
CTACCAATCAAGTTATCACCCTCCTAAATTTAGCCCCAAAACCCTAGAGA13.4482758620689653No Hit
ATTTTAATCCGAATTCAGGAATGTGAAGATTACGTATATTGTATAAGTTT13.4482758620689653No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG13.4482758620689653No Hit
CTCTATGCGAGAGATTGGACCAGGCGAACATGGAAAAGAACATAGTACTG13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)