FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA100.33036009250082593No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.2642880740006607No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.2642880740006607No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA70.23125206475057813No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT70.23125206475057813No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA60.19821605550049554No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.19821605550049554No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.19821605550049554No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG60.19821605550049554No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG60.19821605550049554No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG60.19821605550049554No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG60.19821605550049554No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT60.19821605550049554No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT60.19821605550049554No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG50.16518004625041297No Hit
AGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGT50.16518004625041297No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA50.16518004625041297No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG50.16518004625041297No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG50.16518004625041297No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT50.16518004625041297No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA50.16518004625041297No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT50.16518004625041297No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA50.16518004625041297No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.16518004625041297No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG50.16518004625041297No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA50.16518004625041297No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.16518004625041297No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA50.16518004625041297No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT50.16518004625041297No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA50.16518004625041297No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA50.16518004625041297No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA50.16518004625041297No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA50.16518004625041297No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT50.16518004625041297No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC50.16518004625041297No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA50.16518004625041297No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.13214403700033034No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT40.13214403700033034No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG40.13214403700033034No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA40.13214403700033034No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT40.13214403700033034No Hit
GACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAG40.13214403700033034No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC40.13214403700033034No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC40.13214403700033034No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC40.13214403700033034No Hit
GTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCA40.13214403700033034No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC40.13214403700033034No Hit
GATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCC40.13214403700033034No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT40.13214403700033034No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.13214403700033034No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT40.13214403700033034No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT40.13214403700033034No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA40.13214403700033034No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40.13214403700033034No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC40.13214403700033034No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG40.13214403700033034No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.13214403700033034No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40.13214403700033034No Hit
GGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAG40.13214403700033034No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT40.13214403700033034No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT40.13214403700033034No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG40.13214403700033034No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT40.13214403700033034No Hit
GATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCA40.13214403700033034No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.13214403700033034No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.13214403700033034No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.13214403700033034No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)