FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT13.4482758620689653No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAGGGCAATGAAAGAATATGGGGAA13.4482758620689653No Hit
ACTTCAAGCACTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT13.4482758620689653No Hit
GGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAGCTTGCGCATAGTT13.4482758620689653No Hit
ATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACC13.4482758620689653No Hit
TTCAAATATAAATAAAAGACCAAGGAATTCTTGGAACAAACATCGGAAAA13.4482758620689653No Hit
GGCGATACCAAATTACAGCAGACACGAGAATAATGGACATGATTCCAGAG13.4482758620689653No Hit
CCGTACCATCCATCTCCCATCCCTGTCCACCCCCCTTCAATGAAACCGGC13.4482758620689653No Hit
TATGATAGTTGTCTTATACTTTTTTTAAAATTTATCTAGGAGCATTTGAG13.4482758620689653No Hit
AGTCAATATTAAGACAAAACTCACCCTCATATTAAACTCAGGGCATATTT13.4482758620689653No Hit
ACATTAGCCTTCTCTCCTTTAGAAATATTGCTCAGTTAATTGATGCTTAA13.4482758620689653No Hit
GCGCATCCAGCAGCCTTCCAGGTGCCCGCTTCTGGCAAAGAGGCCCATCA13.4482758620689653No Hit
CTACAGTGCATCTGTGAGGAAGGAGTTGACCAAAGAAGCAATAAGCAAAA13.4482758620689653No Hit
GCGGAAAAGGCAAGGAAAGAATATGCGGAAGATCCGAACATCGAAACTAA13.4482758620689653No Hit
GGGATGAGGGATTACAAGCAGGAGTGGACAGATTCTACCGGGCCTGCAAG13.4482758620689653No Hit
CCTTGGCACTGAGATCTGCATGGCCAGGGTTTGTATCAACTCTCCTCCTT13.4482758620689653No Hit
GGATACACGATGGACACAGTAAACAGAACACACAAATACTCAGAAAAGGG13.4482758620689653No Hit
CAGCAATAGGGGTGAGGATTAGGTCATCTGTGAGGGTTGGTGGGGTCCCT13.4482758620689653No Hit
GGATCATGGAGATGACTACTGGAAGGGTCCCGTGTCCCCGGGCTCTCAGT13.4482758620689653No Hit
CTGGGGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTG13.4482758620689653No Hit
GACTAATCCAGAATATCATAACAATAGAGANGATGNTGNNTTCTNCTTTN13.4482758620689653No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT13.4482758620689653No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA13.4482758620689653No Hit
CTATTTCATCTTTGTTCAATGATTCCAATACATCATTTAAGAAATATATT13.4482758620689653No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13.4482758620689653No Hit
GTCTTCAGGAGCCCTGCCGTGGGCCACTTGTGCTGGAAAATCGTGCCGCC13.4482758620689653No Hit
GCTGCATTGAGGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCAAA13.4482758620689653No Hit
GTCCAGACTCGAAGTCCACCCTGGCATCAATCCGGGCCCTAGACACCATG13.4482758620689653No Hit
ATATTAAAAAATTCGACACAAATTGATCGACATCAGAAATCTATTCGTCG13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)