FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC21.342281879194631No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG21.342281879194631No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT21.342281879194631No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG21.342281879194631No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC21.342281879194631No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT21.342281879194631No Hit
CTTTTAGAAGAGGCCTGATTTTCTCAATTTTCTTCTTTGTTGCTTCATTG10.6711409395973155No Hit
GGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCCCTAAC10.6711409395973155No Hit
GACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGAC10.6711409395973155No Hit
ATACTGAATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGGGA10.6711409395973155No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGCCACTGTCCAAATAA10.6711409395973155No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC10.6711409395973155No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA10.6711409395973155No Hit
CCAGTCGCCGAAAGGTTAGATAGTCAGCCGACAAAAAAGCAAGTTGTTGA10.6711409395973155No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA10.6711409395973155No Hit
TTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAGA10.6711409395973155No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6711409395973155No Hit
CAGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG10.6711409395973155No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.6711409395973155No Hit
GTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCC10.6711409395973155No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG10.6711409395973155No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.6711409395973155No Hit
GACGCATATAGCCTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACAC10.6711409395973155No Hit
GGCTTGTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCA10.6711409395973155No Hit
GGAGGGGCGGTGCCTGGGCGGCCGGCGGCGGGGAGGGGCCGGGGGGGGGG10.6711409395973155No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCT10.6711409395973155No Hit
GGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTC10.6711409395973155No Hit
TTATAGGTGCCATGCTCAGGATGTATCTGAACCACTCGGGTTGATTTCTG10.6711409395973155No Hit
GCAGAAATGCTAGCAAGCATTGACCTGAAGTACTTCAATGAATCAACAAA10.6711409395973155No Hit
TGATGACTCCGAGCGCAAAGAGGCTGAGGGCAGCAGCAGTAGCAAGCTTG10.6711409395973155No Hit
ATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAGTCTGG10.6711409395973155No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT10.6711409395973155No Hit
GTTCGCTTTCCCTAGGCTTGCCATTTTGTTTGAGAACATTATAGGTGCCA10.6711409395973155No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC10.6711409395973155No Hit
CTACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAAT10.6711409395973155No Hit
AAAGTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTC10.6711409395973155No Hit
AACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTC10.6711409395973155No Hit
ATCTGGTGACCCGAATGCACTATTGCAGCACCGATTTGAGATAATTGAAG10.6711409395973155No Hit
GATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAA10.6711409395973155No Hit
AAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATAT10.6711409395973155No Hit
CTCCAGGATTGTAAAAATGCTCCCTGGATGGTGGCGTACATGCTAGAAAG10.6711409395973155No Hit
GAATAAAAGAACTGAGATCTCGAATGACGCAGTCCCGCACTCTCGAGCGA10.6711409395973155No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT10.6711409395973155No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC10.6711409395973155No Hit
GCTGCCATGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAA10.6711409395973155No Hit
GTTTATCATGTTGTTCTTTATCTCTGTTACTCCAATACTCATGTCAGCTG10.6711409395973155No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGCTGGCT10.6711409395973155No Hit
TCCCTTAGCTCCCATTTTAAGCAGACGTCAGGAATGTGTAGATTCCGTAT10.6711409395973155No Hit
CTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTC10.6711409395973155No Hit
AATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCA10.6711409395973155No Hit
AGAACATAATACTCCCGATCCACACAGAGAAACGCAAAGAACTGAGAAGA10.6711409395973155No Hit
ATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTCATTGCCATCATCCAC10.6711409395973155No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC10.6711409395973155No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT10.6711409395973155No Hit
GGTCCAAGGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAAT10.6711409395973155No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGCCATATGCAAGGCA10.6711409395973155No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG10.6711409395973155No Hit
AGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAG10.6711409395973155No Hit
TTTCCAACAGCACTGCTAATGATTAGGCTGTAACTGATGCACATCTTACA10.6711409395973155No Hit
ATGGTATTCCCTCCCCCTCGTGACTCGGGGCTTCAATACTTAATTTCAGA10.6711409395973155No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10.6711409395973155No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT10.6711409395973155No Hit
GGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGAT10.6711409395973155No Hit
GTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAA10.6711409395973155No Hit
GTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGG10.6711409395973155No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC10.6711409395973155No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG10.6711409395973155No Hit
CCTGAATACATAATGAACGGTCTGTACATTACTACGGCCGAGCTCATTTA10.6711409395973155No Hit
CTTTGAGAGGGCAGGGTAGTGCGTGGTCGGGAGTGGGGGCTTGAACTTGC10.6711409395973155No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG10.6711409395973155No Hit
ATGTCGCAGTCCCGCACTCGTGAGATACTCACTAAGACCACTGTGGACCA10.6711409395973155No Hit
GTAAAATAGTTCCTCCTCATGCGTGCGATATGGTCCATCGGGGCACCATC10.6711409395973155No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGATACTCCAAAGCTG10.6711409395973155No Hit
GTTCATTGAAATTGGAGTAACACGGAGGGAAGTCCACATATATTACATAG10.6711409395973155No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10.6711409395973155No Hit
GCGACAGCATGACCAAGAAGATGGGCACGCAAAGCACAATTGGGAAGAAA10.6711409395973155No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG10.6711409395973155No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA10.6711409395973155No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG10.6711409395973155No Hit
CCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGATGATTG10.6711409395973155No Hit
GATTTGAAGAGTATGGTTAGCAAAATGAAGGAGAGACACAACTAATTGAG10.6711409395973155No Hit
AAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATT10.6711409395973155No Hit
TAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTG10.6711409395973155No Hit
TGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTC10.6711409395973155No Hit
CACGACAACCAACAACGACACTGCTGCTACCCAGTTCTAATTCTATATTA10.6711409395973155No Hit
CTATTCCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT10.6711409395973155No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC10.6711409395973155No Hit
GGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAA10.6711409395973155No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.6711409395973155No Hit
AATCAGGGTGGTGGTGGGTCGGACGGCGGCAGAGGGGAGGGAGCAGCTGG10.6711409395973155No Hit
TCATTCCACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTC10.6711409395973155No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTGGCGTCCCACTAACTT10.6711409395973155No Hit
GTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTT10.6711409395973155No Hit
TGACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTG10.6711409395973155No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA10.6711409395973155No Hit
GACGAAAGGAATCCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTG10.6711409395973155No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG10.6711409395973155No Hit
GAATGTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCT10.6711409395973155No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCTCAATTTCCCAGT10.6711409395973155No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT10.6711409395973155No Hit
TAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCACGCGCATGT10.6711409395973155No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC10.6711409395973155No Hit
GAAGTACTTCAATGAATCAACAATGAAGAAAATTGAGAAAATACGGCCTC10.6711409395973155No Hit
GCGGGGGGGTCGGGGACGGGTGGTGTGGTGGGGGGGGGGGGGGGGGGGGG10.6711409395973155No Hit
CACCTAATGAAAGATTGATCACTTCATTGATCCTTAAACCTGCTCAATGT10.6711409395973155No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT10.6711409395973155No Hit
TTTCTGAAGTGGACAGGGCCGAAGGTACCATGTTTCAACCTTTCGACCTT10.6711409395973155No Hit
GCCTTCTCTCCTTTTGCAAGATTGCTAAGTTCATTGATTCTTAATGCTGG10.6711409395973155No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.6711409395973155No Hit
AATCTGGACCCCTCTTGTGGAAAGCTGTCCTCTTGGAATCACTTTCTTTC10.6711409395973155No Hit
GATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAAATGTC10.6711409395973155No Hit
CCCCTAGCACGGAGAGGTCGCGGAGAGGGATATGCGTCAGCAAAATGGGG10.6711409395973155No Hit
GCGCTACTCAGATAGGGGTGAGTGGGCGGGCTCCCCTGAGCCTGGTGCGC10.6711409395973155No Hit
CCCCACTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG10.6711409395973155No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC10.6711409395973155No Hit
GTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAA10.6711409395973155No Hit
AATTGAAGTGGGCACTCGGTGAAAATGTGGCACCAGAAAAAGTAGACTTT10.6711409395973155No Hit
GTACGTCTGCTCCGCGCACGTCCCTTGGGTTTTGTGCAGGACTTCAATCT10.6711409395973155No Hit
CTCTGAGACCGGGCATCCAGCTGACCCCAACCGGGCGTCCAGCCTCTCCC10.6711409395973155No Hit
CCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAG10.6711409395973155No Hit
AAGCACACCAATACCCTCTCCCTGCTGGAGAACTCATCTAAACCCATTTT10.6711409395973155No Hit
GAAGATGCCTGGGCCGAGGCTTGGGTGTTTGCATATCCTGAACTGCCGGG10.6711409395973155No Hit
AGATAGCCTCTCTTCGTCCGTCGTTGGGTGTTCTTCCCGATGGTTCGGTG10.6711409395973155No Hit
CCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAAT10.6711409395973155No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG10.6711409395973155No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC10.6711409395973155No Hit
GGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGG10.6711409395973155No Hit
GGTTTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACA10.6711409395973155No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10.6711409395973155No Hit
CTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGG10.6711409395973155No Hit
CAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCATAGAGGCCCAT10.6711409395973155No Hit
ACCTGAACCACTCACATTCACACTCAAAGAGGAAAATTGCATCCTACTCA10.6711409395973155No Hit
ATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGAGCTGAACT10.6711409395973155No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.6711409395973155No Hit
TTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTC10.6711409395973155No Hit
TTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTTCT10.6711409395973155No Hit
CTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAG10.6711409395973155No Hit
CCTCAAATGATATCAAAAAATATGGATAGCCTAAGGCAAGTGTTCATCAT10.6711409395973155No Hit
CAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACT10.6711409395973155No Hit
GGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTG10.6711409395973155No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATTTGTAGTTGCAACGGCA10.6711409395973155No Hit
ATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTTCTATGTTTCTCGCA10.6711409395973155No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers