FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGGAATCATTAATCAGGCACTCCTGGATTGCTTCAGTTAGCCCCCAA13.7037037037037033No Hit
GTCCACTTCAGAAATCAAGTGAAAATAAGGAGGAGAGTTGATACAAACCC13.7037037037037033No Hit
GTACAAACAAGGTCGGTTTTAAACAATTAGACAATAATTGATGGCCATCC13.7037037037037033No Hit
GGAAGACTTNGTNNNNCNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNN13.7037037037037033No Hit
ATGCACACACAGCCTGTGGGATAGCGTCTCTGGCTTTCATTTACGACGAC13.7037037037037033No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13.7037037037037033No Hit
CAAGTAAGGTCCTGCACAATTTAAAAATATAGCCTAACTCCACACCCCAC13.7037037037037033No Hit
ATGTAGATGGATTCGAGCCTAACGGCTGCATTTAGGGCAATCTTTCCCAA13.7037037037037033No Hit
GATTTGATGAGCTTTGGGTATGAATTTCCTTTTTTATCTAGCCATATTAA13.7037037037037033No Hit
GGACCGAGAGCGGGAACAGCCCCGAGTACTGGCCCGTCTACCATGAGGGA13.7037037037037033No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT13.7037037037037033No Hit
GGGACAAGTAGGTGTCCCTAAGCTATTCAACTCGTGCACTTGCAAGTTGC13.7037037037037033No Hit
AGCACCGAATCCCACGACTATACTGGCAAAAACTGAATTAAACAGTCTAT13.7037037037037033No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC13.7037037037037033No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.7037037037037033No Hit
GCAATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGACCCCCGCAGT13.7037037037037033No Hit
GAGTTGATACAAACCCTGGCCAGGCAGATCTCAGTGCCAAGGAGGCCAAG13.7037037037037033No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT13.7037037037037033No Hit
AGAACAAACAATGTCAAAATTAAACAATACGAGACTAATTGAACTCCATC13.7037037037037033No Hit
CTATTAGAAGAGGCATTATTTTCTCAATTTTCTTCTTTGTTTATTAATTG13.7037037037037033No Hit
CCCCCCAAGCTCGAAGGTTCCAGGTTCCAGGTTGTCGCTAAGTGCCTGAA13.7037037037037033No Hit
CAAGAAATAAAAATACAATGATAAACACCAATCATCGACCATCTAGCGGA13.7037037037037033No Hit
AGTAGAAACAATGTCGTTTTTAAACAATTCGACACTAATTGATGTCCATC13.7037037037037033No Hit
CAGATTAACAATTCAAAAGTATTGACTATCACTGACTCAGCGCCATAGAT13.7037037037037033No Hit
AAGCAAGACCCAGGGGTCATTTATCAGGCACTCCTCGATTGCTACCTATA13.7037037037037033No Hit
ATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACA13.7037037037037033No Hit
TATTCTCATTGCACCAACTACTTAAGTACAGTGGGGACGAATGAGGTGAA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)