FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC210.526315789473683No Hit
TTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACAGGG15.263157894736842No Hit
GTTGAGAATAGAACGCTTCCTCTTGGGACTCCAGGAATGTGTAGTTGCAA15.263157894736842No Hit
CCTATATGTGAGAACCAATGGAACCCCCAAGATCAAGATGAAATGGGGCA15.263157894736842No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG15.263157894736842No Hit
CAACCACCTGGAAAAAAGATTAGAGAATTTAAATAAAAAAGTTGATGATG15.263157894736842No Hit
GTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGAAACGAGACTGGA15.263157894736842No Hit
TCTTTGGTCATCGTATATAATGTCAGGGCTATTATTAGATAGCCTCTCTT15.263157894736842No Hit
TCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGT15.263157894736842No Hit
CTTCTGGTACATGTGGTCGTCCTCAAGCAGTCCCCGGTGGCTTGTGTTGA15.263157894736842No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC15.263157894736842No Hit
ATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAAC15.263157894736842No Hit
GAAGAGGCAGCACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG15.263157894736842No Hit
ATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAAT15.263157894736842No Hit
TCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGA15.263157894736842No Hit
ATGCCATCCACACCAATTGACTCTTGTGAGCTGGGTTTTCATTTGGTCTC15.263157894736842No Hit
TAGTATTCCCCTCACAAGCTGAGACAGACTTATAGAGCTAGAGCAGGTGA15.263157894736842No Hit
CTAGAGTCCCGTTTTCGTTTCATTTCCAACACTACGTCCCCTTTCCCAAT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)