FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT15.263157894736842No Hit
GGAGAGGAGCTGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG15.263157894736842No Hit
GTGTAACACAACAGGGGTAGATAAGCCGTCATGTGGGCCGGAGTTGTCTG15.263157894736842No Hit
ATGCACACATTTGGAAGTGTGTTTCATGTATTCGGATTTCCATTTCATCG15.263157894736842No Hit
TAGGTATATATTTCTTCCAATACATGCGCATGGTGTCTTTTCCCTTTTGG15.263157894736842No Hit
CCTCTTACCAGTATCCTCACGCCTGATACTCTAAGATTCACAGTCAATGA15.263157894736842No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATACCCGCAAGCGATT15.263157894736842No Hit
CAATAGTACGGTCCCTATTTGATCTCTAACACTTAAAAATCGGTCAATAC15.263157894736842No Hit
GTATCAAGGTCTCTAATCGGTTAAAGGTTACACTGAAGTTCGCTTTCAGT15.263157894736842No Hit
ATACTCTCATGACAGCAGACTCCACCCCAGATGTTCCTTCATATGGAGCT15.263157894736842No Hit
AGTAGAAACAACTCAATAAATAATGAAGGACAAGTTAACTTCAGTAAAAG15.263157894736842No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT15.263157894736842No Hit
GGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15.263157894736842No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG15.263157894736842No Hit
GGGCTATGGGGGGGGGGGGGGCGGCCGGGGGGGGGGGGCCTGGGGCCGGG15.263157894736842No Hit
AAACAAGGTCGTTTTTAAAAAATTCGACCCTAATTGATGCCCCTCCGAAT15.263157894736842No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC15.263157894736842No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT15.263157894736842No Hit
CCACTGGGTGAGTCGGCGATTGGCCATGTTGCCGAGTTGTGATCAAAGAA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)