FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGAATTCTTTTGGTCGCTGTCTGGCTCTCAGTAAGTATGCTAGAGTCA13.8461538461538463No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA13.8461538461538463No Hit
GGTAAGGACAGCACTCTCATATTCAACTAAAACTCCAAAAGAAAGCTTCC13.8461538461538463No Hit
ACAATAGACTGAAATGTTTCAAATTCCATTTTGTTGTATAAAATTGTTGG13.8461538461538463No Hit
CATATATTACCTAGAGAAAGCCAACAATATAAAATCTGAGAATACTCACA13.8461538461538463No Hit
CCCTATTGCTTAATTGAATATGTCTACGGCTTGTTCCTCATTTGTATTCT13.8461538461538463No Hit
GAATGGTAGAGGGGGCGGGAAATGGGTAGACATGCAGGACAGGCGCCAGG13.8461538461538463No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA13.8461538461538463No Hit
AAATAAAACAAAATTCTAAAAGGTCGAAAGGTTGGAACATCTTACCATCT13.8461538461538463No Hit
CTCCAGCGCTTCCATTTCCTTCCATCATCTCTTGCATGGATTACTACAGC13.8461538461538463No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA13.8461538461538463No Hit
ATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACA13.8461538461538463No Hit
CCTTCTACGCATGTCACAAAACCATCATTTAATCAGAACTCATTTGTTTC13.8461538461538463No Hit
CTCGAGGAAATGTCACGAGACTGGTTCATCCTCATGCCTAGGCAAAAGAT13.8461538461538463No Hit
GGGAGAAGCAGGCTCCCTGCGGAGTGAGGAGACCCATGTGGGACTCCATT13.8461538461538463No Hit
ATGTATCACATACTGTATCCTTACAATAAAGTAAGCGAGAGAAAAGAAAA13.8461538461538463No Hit
GAACAGCACATCTCACTTAACTTGTTATAGGCCTCGACTTAATCATATAT13.8461538461538463No Hit
ATAGAATGATGGTTACCCGGGCCTAAAGGGAGGTGGTCATGGGGAGTTAC13.8461538461538463No Hit
GGATACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT13.8461538461538463No Hit
GGATCATAGGTCTAAGCGGGCACTTCTTGTGTGGCTGACAAGCTGCTCTA13.8461538461538463No Hit
ATCACGCAATACTCTATTGATTCATATATCACCCCAATATCTAATGTTCC13.8461538461538463No Hit
GTTCAGATCCCCCCTGACTGCCTTGATCATGCAATCCTCCTGTGAGAGAA13.8461538461538463No Hit
ATCCGCGCGCTTCACTGCTTCGGGTCCTGAAACCAACAAATTCCAACGGC13.8461538461538463No Hit
ATGTGCTACACAAAACTTTAAGTTGAACAATTTCAGGCTCTTGTCCATAA13.8461538461538463No Hit
GCCTTGGTTTCCTCACCTGTAAACCAGAGATCACAAAGCCTTCCTGAGAG13.8461538461538463No Hit
GGATCACTCATCTTTATTTCCCCCCCAAAGCACCTGCGAGTCTGCCTTTT13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)