FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG32.272727272727273No Hit
GCCATAATGGTTGCTCTTTCAAAAGGGAGATTCCGCTGCACTGAGAATGT10.7575757575757576No Hit
TATGTATTCAGTAGCCCTGCAGTGGGACCCTTCTGCTGTAAAATAGTTCC10.7575757575757576No Hit
GTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCA10.7575757575757576No Hit
GGGGGGGGGCTTGGGGTGGGGGGTGGGGGGGGGGGTGGGGGGGGGGGGTG10.7575757575757576No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC10.7575757575757576No Hit
GTAAAAGGGTTTTCATTCAAATACGGCAATGGTGTTTGGATAGGGAGAAC10.7575757575757576No Hit
GCCTGCCAGTCGGCGGCGTTGGAAGGGGTAAGTGTAGCACGGTGGAGAGG10.7575757575757576No Hit
GTGTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCC10.7575757575757576No Hit
ACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAG10.7575757575757576No Hit
AGCTTGCGCATAGTTCCAGTAATCTCAAATTTTTATTCAATTGTCTCTTC10.7575757575757576No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC10.7575757575757576No Hit
AGATGGCAGTGAGCCGACGGACTGGCAACAAGGCTCTCCAGGTGAATAAA10.7575757575757576No Hit
TGTTCATTGATTCCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGAC10.7575757575757576No Hit
CTTCAATACTTAATTTCAGAGCATCCATCCGAAGGAACTTTGACCGCTGA10.7575757575757576No Hit
GGGGGGGGGGGGGGTGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG10.7575757575757576No Hit
CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAA10.7575757575757576No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC10.7575757575757576No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT10.7575757575757576No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC10.7575757575757576No Hit
GGAGTAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCG10.7575757575757576No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT10.7575757575757576No Hit
ACTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.7575757575757576No Hit
ATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCC10.7575757575757576No Hit
CTAAGTGGAATTTGAGGCATTTCAGGCTCTTGTCCCTAAGGCCGCCAGCG10.7575757575757576No Hit
GATCAGTTGCACATAAATCCTGCCTGCCTGCTTGTGTGTATGGGCTTGCA10.7575757575757576No Hit
AAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATT10.7575757575757576No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG10.7575757575757576No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA10.7575757575757576No Hit
GTCCCCCTCTCCCTCCCTGTCTCAGCCACACTCCTGTCACACGTGCCATA10.7575757575757576No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG10.7575757575757576No Hit
ACACTAATTGATGGCCCTCCGAATTCTTTTGGTCCCTGGCGGGCTGTCAC10.7575757575757576No Hit
GCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAA10.7575757575757576No Hit
ATGCAATTACCTCCTTTAATTTTTCTATTTGGATTGCTGACGCTGTCGCT10.7575757575757576No Hit
CGTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGATAGGCCTAGTGTC10.7575757575757576No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG10.7575757575757576No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC10.7575757575757576No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.7575757575757576No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.7575757575757576No Hit
CACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTT10.7575757575757576No Hit
AAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCGTATAC10.7575757575757576No Hit
GGGTAGGGCTGGGGCGGGGGCGGGGGCTGGGGTTGGGGCATGGGCGCCGT10.7575757575757576No Hit
GGCTCGCGCTGTGGGGGGGGGTCGGGGTGGGGGTGGGGTGGCGGGCGGGG10.7575757575757576No Hit
AACATGGGCCTCGACACTTGTCCTATCGCAGTCCTCAAGAGCATGTCTCC10.7575757575757576No Hit
CAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGA10.7575757575757576No Hit
GGTCAACATCATCATTTCTCACTTCTCCTCCTGGAGTGTACATCTGCTCC10.7575757575757576No Hit
GTTCCATTGGTTCTCACATATAGGAACATGGGCCTCGACACTTGGCCTAT10.7575757575757576No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC10.7575757575757576No Hit
CTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGAT10.7575757575757576No Hit
TTGATGAACAATTGAATAGCCATCTGGGCCGGTGCAGGTCCAAGGTCATT10.7575757575757576No Hit
TTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCGT10.7575757575757576No Hit
GACAAGGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAATTTC10.7575757575757576No Hit
GGGCAGTGAGGGGGGGAGTTGGGGGAAGGCGGGGCCCGGGAGGGGGGGCG10.7575757575757576No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT10.7575757575757576No Hit
CCCCCCCCCATGCAATGTGCATCCAGTAAGCACCAACTGTGTACTTGGGA10.7575757575757576No Hit
CCTTTTGCAAGATAGCTCAGTTCATTAATCCTAAATGCAGAGCCATCTAA10.7575757575757576No Hit
TAAATAGAGATAACACCCCACTTGCACAGAAAAAGGAGAGTAAGAGACCA10.7575757575757576No Hit
GTCCCTGTTTACCCCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTG10.7575757575757576No Hit
CATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAATTTT10.7575757575757576No Hit
CCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGA10.7575757575757576No Hit
AGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCAT10.7575757575757576No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGGCTTCACCGAGAA10.7575757575757576No Hit
GTACATTACAAGGTCCATCGGTCATTACAGAAAAGCAAGAGGCATTTACA10.7575757575757576No Hit
GTCGAGCTTGCGGAAAGGGAAAGAATATGGGGAAGATCCGAAAATCGAAA10.7575757575757576No Hit
TGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTAT10.7575757575757576No Hit
GTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT10.7575757575757576No Hit
ACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAG10.7575757575757576No Hit
AAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGAT10.7575757575757576No Hit
CAACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTACTGGGAGCCCA10.7575757575757576No Hit
GTATGGCAGTNNNGGNNNNNNNGCNNNNNNNNNNNNNNNNNGNNNNNNNN10.7575757575757576No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC10.7575757575757576No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.7575757575757576No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA10.7575757575757576No Hit
CTGACTTTCATTTGTTCTGACGGCTTTTAAAAACTATTAAATAAGAAACA10.7575757575757576No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.7575757575757576No Hit
GCCCAGTATCTGCTTCTCAGTTCCAGGGTATTGGAGGCCATGGTTTCCAC10.7575757575757576No Hit
GACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGAT10.7575757575757576No Hit
CATTTGGAAGTTTGTTTCATGTATTAGGATTTCCATTTCATCGCCGACCG10.7575757575757576No Hit
TCTCAGTGCAGCGGAATCTCCCTTTTGAAAGAGCAACCGTTATGGCAGCA10.7575757575757576No Hit
TCATTCCACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTC10.7575757575757576No Hit
GTCATACTGTTTAAGGTCTCCAACAACTTTGCAGTCATCAAAGTATACTT10.7575757575757576No Hit
TGGTCGGTGTGGAGGCACGCCGCTTCGGATTTCTGGCTGCCTCCTATGCC10.7575757575757576No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCA10.7575757575757576No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTG10.7575757575757576No Hit
GTATTCATCTACTCCCATTTTGCTGCCTCTTATCCCTCTCAGCGCCATCT10.7575757575757576No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT10.7575757575757576No Hit
GCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAA10.7575757575757576No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT10.7575757575757576No Hit
ACTGATTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCT10.7575757575757576No Hit
AACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAA10.7575757575757576No Hit
CTTACAAGCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGA10.7575757575757576No Hit
GACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAG10.7575757575757576No Hit
CTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAG10.7575757575757576No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA10.7575757575757576No Hit
GTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAG10.7575757575757576No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT10.7575757575757576No Hit
GGTCGAAAGGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATC10.7575757575757576No Hit
CCTCCTATGAAACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCA10.7575757575757576No Hit
GTTCAGAAACATCCTGGGCATGGCGCCCATAATGTTCTCAAACTAAATGG10.7575757575757576No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG10.7575757575757576No Hit
GAGTAAAATCAAGTGGTAAAATAAATTAACCAGTAAGTTCAAAAACAGGA10.7575757575757576No Hit
CACACACACACGGCCAGGAACGCCTGCTTGTGTACTCATATGCACACCCA10.7575757575757576No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC10.7575757575757576No Hit
AACCAATACTCATAAACTGGAAAGTGGAAGACAAACACAGAGACTGGGGC10.7575757575757576No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC10.7575757575757576No Hit
GGGGTGGGGTGAGGAGGGGCATGTTGCACATGCTACGTAGGGTCGTGGGA10.7575757575757576No Hit
TTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAGGTAGT10.7575757575757576No Hit
AAAGCACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA10.7575757575757576No Hit
ATTACTACCAAAGTTTATTCTGTTATTGAAAAGATGAATACACAGTTCAC10.7575757575757576No Hit
GTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAG10.7575757575757576No Hit
CTTGTATAGTTCCAGGCCCACCAGGCCTGCCACAGCTGCTGTGGTGGTGG10.7575757575757576No Hit
AACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATTAC10.7575757575757576No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA10.7575757575757576No Hit
GTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTC10.7575757575757576No Hit
TCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACC10.7575757575757576No Hit
AGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGC10.7575757575757576No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGCCAAAAGCAATACCCCAAGCC10.7575757575757576No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT10.7575757575757576No Hit
GAGAGGCAGTAGGCAGGGGGTTTAGAGAGGCAGAGCAGGGAGCCCAAAGG10.7575757575757576No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC10.7575757575757576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG10.7575757575757576No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7575757575757576No Hit
AGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGC10.7575757575757576No Hit
TACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCATACAC10.7575757575757576No Hit
GACAAAAAGAGACTATCTAATATCTACCGTATTGTACTTTGGCTCACGTT10.7575757575757576No Hit
GAAGAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC10.7575757575757576No Hit
ATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTT10.7575757575757576No Hit
ATAATGTGTGTCTGTTTAGGGTTTGATCCAATACTCATGTGAGATCAGTC10.7575757575757576No Hit
CACATACATTTGCATACATTTTACATTTTTATCTACAAAGGCTTGAACAT10.7575757575757576No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7575757575757576No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers