FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTG11.5625No Hit
GTTTTGCCATATTGACAAAGTTCAGATAGCCCATAACTTCCTTGAACATT11.5625No Hit
TAACACAACAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT11.5625No Hit
GAATGGATAGCAAATAGTATAATTCCCACCACTAATTCAGTTCATGTGTG11.5625No Hit
GATCTAATGTCGCAGTCACGCACTCGCGAGATACTCACTAAGACCACTGT11.5625No Hit
GTAATGGATTGAATGGACTTAGAGGAATTAGAGATAGCTAGCCCCTTTCA11.5625No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCAACAAATCCATAGCCA11.5625No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCAGTCAGTAAGT11.5625No Hit
GTGCCTGAAGAATGAGAAGGAATTTTCTACATTCAGCCGAAAACCCCTCA11.5625No Hit
GAGTGCTGTTGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACT11.5625No Hit
CCTTATTAGTATCAAGGTCTCTAATCGGTAAAAGATTACACTGAAGTTCC11.5625No Hit
GGTGACCCGAATGAACTATTGAAGCACCGATTTGAGATAATTGAAGGAAG11.5625No Hit
AGTAGAAAAAAGGTAGTTTTTTACTATAGATATATCTTGACAAAATGAAC11.5625No Hit
GGTCCAGGAGGGAAGTCAAGCGGGAGGGGACAGATTCGCCAGGCCCTGCC11.5625No Hit
CACCACATCAGTATCATTTCTCAATTGAGACCGTCCTGTTAAAATGAACC11.5625No Hit
TAATTATGCTAGAGTCCCGTTTTCGTTTAATTACAAACACAACGTACCCT11.5625No Hit
GTCCCATAGGTGTGCAACAAAACTGATCTGGAGATGCCATTTGTATGATT11.5625No Hit
ATGTGATGTGAATGATCGGAATCCTGCCCGACATGCACCCAAGCACGGAG11.5625No Hit
ATATACTTAAATAATCCAAACAGCCAATAAAAACGAATAACCAACACAAT11.5625No Hit
TATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTC11.5625No Hit
GTTCTATCCAGCTTGAATCAGTCACTTCACATGCCTTATTGAATTCATAT11.5625No Hit
CCATATCGCCATAAACAAAAAGTACACATCAGGAAGCCAAGAGAAGAACA11.5625No Hit
CAGATAGACGCTATCAGAACTAAGTCAATAGGCAAAACCACCGACTACAC11.5625No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG11.5625No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT11.5625No Hit
AACTTAAAAGTTCATCCATACGATTAAATCTCTCATTCCACCTATTGACT11.5625No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGAACAGACCAGACTCGA11.5625No Hit
GTCCAAGATTCAGTATCGAGACTCAAAATCCCGTACTTAGCATGTTGACC11.5625No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11.5625No Hit
CATAGGGATCAGTGCGGACGCAACTGGCCGGCATTCTGAATAAGTTCTGT11.5625No Hit
ACTTAGTGTTGTCACAAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11.5625No Hit
ATACCAGACTCTTCAAGCTATTCGCAAATCCACCTAGATAAAGTATCAAC11.5625No Hit
CCTCAATACCATGAAACATTTCATATTGAACTTGTATGTTCCAATGGTAA11.5625No Hit
TTATACCCCATTGCTGTTTTCAACCAGTCGACCCAACTTTCAGTAACTCC11.5625No Hit
GTTCTAAAGAAACAAATGGGTCACATTCCATTTTAATGTATAGCATAGTA11.5625No Hit
GTGTTGCACTTAACCCAAGGGACGTGCTCGGAGCAGATGTACACTCCAGG11.5625No Hit
CAGGCAATCTGAGGCGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTG11.5625No Hit
ATTCCTGTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTT11.5625No Hit
ACACAGTCAGATTGTGCATACACACTTGGTTCATTATCCTCAGGTAGTGG11.5625No Hit
GATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACA11.5625No Hit
AAGTATGCTAGAGTCCCGGTTTCGATTCATTAACAACACAACTTACCCTT11.5625No Hit
GATCTCTGGTTCTACGTCCAGCAGTAGTTTTCAGGCTGGCAGAAATGTTC11.5625No Hit
GAACGAATGATCCAGGTTCAATCATTTCAATAAGTGACTTAACAATGAGA11.5625No Hit
GTATCAGATGGAGGCCCAAACTTATACAATATACGGAATCTTCACATTAC11.5625No Hit
GGATCGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAATATAGCAG11.5625No Hit
CATATAGGTGCCATAGGGGAGCCACACAAATTCAGACGAGAAGATCATTT11.5625No Hit
GAACCATAACCATCTCGGTTGGAAATGTGCCGCCGCACACAGCTGGGAGG11.5625No Hit
GATACCCATATAGTTGCCATAGGGGAGACACACAAATTCAGACGAGAAGA11.5625No Hit
GAGCATGATTGAGGCCGAGTCTACTGTCAAAGAGAAAGACATGACCAAGG11.5625No Hit
GGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAG11.5625No Hit
TCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTT11.5625No Hit
GATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGG11.5625No Hit
GATGATGGGCATGTTCAAAATTCTAAGTACGGTCTTTGGAGTCTCGATAA11.5625No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11.5625No Hit
CTATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGA11.5625No Hit
TGGCTGCTCAGAAATTAATTATTGAAGACAAGACACACGAGGGGGAGGGA11.5625No Hit
CTGCCTGGCTGTCAGTAAGTATGCTAGAGTCCAGTTTTCGGTTAATGACC11.5625No Hit
AAACAAGGTCTTTTTTAAAAAAAACTAAACTAATTGATGGCAATACTAAA11.5625No Hit
CTCTTAGCTACTCATAATCGATGAAATCTCCAGGGTAACACGTTCCATTG11.5625No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT11.5625No Hit
GGATGATGGCAATGAGATACACAATTACAGCAGACAAGAGAATAATGGAC11.5625No Hit
AGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT11.5625No Hit
GTCCTATATAAATAACACAGGGACATTTCAATTCAGAAGCTTTTTTTATC11.5625No Hit
AACTTCATTTACTCTTATTTTCAGTGTTTGTAGGTTTAACGTTAGCACTT11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCC50.0145.02
TTTCATT50.0145.0145
GTTTTGC50.0145.01
TGCCATA50.0145.05
CCATATT50.0145.07
GCCATAT50.0145.06
ATATTGA50.0145.09
CATATTG50.0145.08
TTTGCCA50.0145.03
TTGCCAT50.0145.04
Produced by FastQC (version 0.11.5)