FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA40.3436426116838488No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.3436426116838488No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.25773195876288657No Hit
CCTTGAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTC30.25773195876288657No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATCCTCAAG30.25773195876288657No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.25773195876288657No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.25773195876288657No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.25773195876288657No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.25773195876288657No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC20.1718213058419244No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT20.1718213058419244No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC20.1718213058419244No Hit
ATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT20.1718213058419244No Hit
CTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCA20.1718213058419244No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA20.1718213058419244No Hit
CAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCCTGC20.1718213058419244No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC20.1718213058419244No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT20.1718213058419244No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG20.1718213058419244No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA20.1718213058419244No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.1718213058419244No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG20.1718213058419244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1718213058419244No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.1718213058419244No Hit
AGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGA20.1718213058419244No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.1718213058419244No Hit
AGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGC20.1718213058419244No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20.1718213058419244No Hit
AACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGG20.1718213058419244No Hit
GTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCT20.1718213058419244No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.1718213058419244No Hit
ACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTCACATC20.1718213058419244No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.1718213058419244No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.1718213058419244No Hit
CATTCTGAGGGGATCAGTTGCACATAAATCCTGCCTGCCTGCTTGTGTGT20.1718213058419244No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT20.1718213058419244No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC20.1718213058419244No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.1718213058419244No Hit
CCCACATCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTTGC20.1718213058419244No Hit
GGGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCT20.1718213058419244No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.1718213058419244No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT20.1718213058419244No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG20.1718213058419244No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA20.1718213058419244No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)