FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13.7037037037037033No Hit
TCCATACAGCCATGGAACAGGAACAGGATACACCATATTCGAGAAATTTT13.7037037037037033No Hit
ATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCCTGAAGTC13.7037037037037033No Hit
CCCATATATAGCAGAGTAGACGGAAAGTTGATGAGAGAACTCATCCTTTA13.7037037037037033No Hit
TCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCCGTGAAATTA13.7037037037037033No Hit
GTCAAGGGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAATGAAA13.7037037037037033No Hit
ACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATT13.7037037037037033No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT13.7037037037037033No Hit
TCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCT13.7037037037037033No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA13.7037037037037033No Hit
GACTAAGACCACTGTTGACCATATTGCCAAAATAAAAAAATACACAACAG13.7037037037037033No Hit
CCAGCAACGGAGCACATGGGTCTTCAATTGTTCAGCATAGCCAACAGATA13.7037037037037033No Hit
GGGCGGGGCGCGGGGTGGGGGGGGGCGGGGTGGGGTCGGGCGGGGCGCGC13.7037037037037033No Hit
TCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACC13.7037037037037033No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA13.7037037037037033No Hit
GTATGCTAGAGTCCCGGTTTCGTTTCATTACCAACACTACGTCCCCTTGC13.7037037037037033No Hit
GAAATAAACCGGCAGAAAAATAATTGGCCAACCAAATAGAACTATTTAGA13.7037037037037033No Hit
GCCTCAAGAGTTGGTGCAGGGGGGTCCGTCGCGGGTTTGCCCTATTGCCA13.7037037037037033No Hit
CCCAGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTA13.7037037037037033No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA13.7037037037037033No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13.7037037037037033No Hit
GCCCATGAACATGCCTCCACTGAGAGATCCACACCCATCGATATCGACAC13.7037037037037033No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA13.7037037037037033No Hit
AGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTATATGCGT13.7037037037037033No Hit
GACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCAT13.7037037037037033No Hit
ATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTTTGACAAG13.7037037037037033No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)