FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG14.3478260869565215No Hit
TACTTAAGTAATTTGCATAGAAGAGCAGATAAGTAAGCATCACTGCTATT14.3478260869565215No Hit
CAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAG14.3478260869565215No Hit
CCGCCCACCCCAACCCCACGCACGTCCTGTGAGGTGTTCCTCTACTGGTG14.3478260869565215No Hit
CTACATGCCAGGCAATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAG14.3478260869565215No Hit
GCATCAGCAAAGTCTTCAGTACTGCCTGTCACATCAGCTAAACCACCTTC14.3478260869565215No Hit
GAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTG14.3478260869565215No Hit
GTCAAAGGATGTTCTTGTGCAGGTTAGTCAGTACCATAAGAACCACACTG14.3478260869565215No Hit
ACCAAAGTGTGAGACTCACGTTACCACGCTTGTCTGGAAGCCAACCCAAA14.3478260869565215No Hit
AGATTCAGATGACAGGGACCATGCTGTCCCATTCTACATCTCCTCAAGAC14.3478260869565215No Hit
GTGGACAGCAATGCCCTCGGCACCATCAAGCTCCGCTTGGAAGTCACATG14.3478260869565215No Hit
TCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAG14.3478260869565215No Hit
GGGGGGGGGGCGATGCGCAGGTCTGTGGTTTTGGTACCTCTGGTTGGAGG14.3478260869565215No Hit
ACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACT14.3478260869565215No Hit
GAAAAAGGTTGACTAAAAAATTGATACAACGAGTCCCCCGAACATAATGC14.3478260869565215No Hit
CAAAGGTTCANANTNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNN14.3478260869565215No Hit
AATTACAGATCAAATGTCAAAGTCCCGAAAACGCGACAAACACAATAAGA14.3478260869565215No Hit
GTGTGCCATGCCTTCCACCCAGCCCCCCCTTAAGTTATAATAATATATTC14.3478260869565215No Hit
ATGCAATAAAATCTTACACCCCTGAAATCCCACTACTCACCAAAAGGCTT14.3478260869565215No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTCTCTTGTCTTCTTTGCCCAAA14.3478260869565215No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA14.3478260869565215No Hit
TAAAGAGACTGGGCAACTCTAAGACTGAAGAAAAGGAAAGGGAGTGGGCA14.3478260869565215No Hit
GGAAATGCTTACTCTTGATTTAGGCTCAGGGCATGATTCCCAGGGTTGTG14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)