FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R2_D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGGGCTGATCAGTATTTGCAATTAAAAATGTATCCATGCACATGTC11.1904761904761905No Hit
GTCTCGCTGTCAGTAAGTATGCAACAGACCCGTTATGGAATAAATAACAA11.1904761904761905No Hit
GCTTCAAGCTCATGCCTAGGCAAAACAAAATAGGCCCAATATTCGTGCGA11.1904761904761905No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGAAACTCACTAAGGATGATGGC11.1904761904761905No Hit
ATATCTCCCTATTCCAACATGTTACGTCCAGAGGTGATACCATAACACTG11.1904761904761905No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.1904761904761905No Hit
ACCTACTACAGCATGCAGGAACAGATGAAGCAGCTGCTAGGAGAACAAGA11.1904761904761905No Hit
CAAAAGGATTGGAACAGTGACCACAGAAGCAGCTTTTGATCTAGTGTGTT11.1904761904761905No Hit
ACCGTAGAAGGCCCACTTTTCAAACCCTAAATAAAGCGACGAAAAATAAA11.1904761904761905No Hit
ATAAGCACAATAGCCTTCTCTCCTTTTGCAAAATTGCTCAGTACATATAT11.1904761904761905No Hit
TGGTAACACTGTTAGAGTCTCTGAAAATATACAGAGAATCGCTTGGAGAA11.1904761904761905No Hit
TCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTCAATACTGGAG11.1904761904761905No Hit
ACTCAACTATTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACAACAAT11.1904761904761905No Hit
GCCCCAGCCCTGAATCTAGTATCGTGAATACTCATTTAGAAGGAACAACC11.1904761904761905No Hit
GAACTGAGAACTTGACAATAACTTATTCAACATCAAAGAAGTGGCAGATC11.1904761904761905No Hit
ATAAAAAATAGAGAGAATAAAAGAAATGATAGATCAAATGTCGCAGACAC11.1904761904761905No Hit
CTCTTAGCCAGCGATTGGACCACGAGATAATGGAAAAGAACATAGTACTG11.1904761904761905No Hit
ACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGA11.1904761904761905No Hit
ATATTACTGGGTCGGATTCTTCAAGGAAAGCAATAGCCTCTAGAACACAT11.1904761904761905No Hit
CCACAACATTCATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTTGCG11.1904761904761905No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTATTCGGCTGAATCTAGAAAA11.1904761904761905No Hit
CACTTGAATCCATGCATCAGCACTCCCATTCGCTTCTGGTAGGCCTGCAA11.1904761904761905No Hit
TCGTCCAATTATGGCGAACACTTCATTAGACAATAGGGAAACCTATAAAT11.1904761904761905No Hit
AACCAGAACACAGAAGGATTCAATTTTCATAATTCACTGTGAAACTCAGA11.1904761904761905No Hit
TGATTATCAATTGATAAGTGTTGACTAGCACTGACTCAGGGCCATTGCAC11.1904761904761905No Hit
AACAGACTGTGTTCTAGAGGCTAAGCCTTTCCTTGAAGAATCCCACCCAG11.1904761904761905No Hit
TAAGACAGCAGACAAGTCAGAGCTATGCCAGTCTGAGGCAGGACGCTCAA11.1904761904761905No Hit
ACTCCCAACCATAGTGAATTCTTCATACCCTTCATGTACTCATATTTTCA11.1904761904761905No Hit
GTCCCACGTTTGAGGGCAAAAGGTGACAGAGGCCGTGAGCATAGCGCTAA11.1904761904761905No Hit
ACATCATCTCAGAGAACTCAACTTACTAGATCCCTCCACACACGAAGTCG11.1904761904761905No Hit
ACTGTCCACAATAAAACCATAATAAAAAAGTACAAATCAGCAATGAAAGA11.1904761904761905No Hit
CGTGGTCACGGCATAGCCAGCAGCCATCTGTTAGTCCGCGCCAGATTGAC11.1904761904761905No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA11.1904761904761905No Hit
GGCCATGTGTGTGAGCGCGTGTGTGCGTGTTTTGTATTAAAGAAAAAAAC11.1904761904761905No Hit
GGATCTTCTCTTCATTTTCAATGACCTGTAGCTCTGCTAGCACCTCCGTC11.1904761904761905No Hit
GGGCGTGGTGTCGTCCAAAAGAATGGTTCAATTTTGGCGTTAACTACTTT11.1904761904761905No Hit
AGCTAATCCTCAACCCTAATGCAGCCTTGCATATGTCTACGGCTTGTTAC11.1904761904761905No Hit
AGAAAACATGAATCGCTGCATCTCCACTCCCATTCGATTCTGCTAGGCCT11.1904761904761905No Hit
CTCTACGCCCTGGTCAGATGACGGCTCTGGTGGGGCCCAATGGCTCCGGG11.1904761904761905No Hit
CACTTCATTTGGGAAAACAACTTACATAATCACATCCTGTGCCTCCTTGG11.1904761904761905No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTATCCTTTGGCATATCC11.1904761904761905No Hit
GGCTAGCTCCAAGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC11.1904761904761905No Hit
CCTCAGAAGAAAGGGAGGGACCCCGGTGACCATTTCCCCAGAGAAGAAAC11.1904761904761905No Hit
AATCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGAAG11.1904761904761905No Hit
CACCTATTGACAATGTTCAGATCGCCCCTAACTGCCTTGATCATGATATC11.1904761904761905No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACATCACTCT11.1904761904761905No Hit
GCCTTTATTGTTTAGGTAATTAAAAAAAGAAACCATTAAAAACATACTAC11.1904761904761905No Hit
TCGTTGTTGATTCATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCT11.1904761904761905No Hit
GCAGGCCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTA11.1904761904761905No Hit
GAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATA11.1904761904761905No Hit
AGCAAAATCAAATCAAAGTCACCTCTGGCAATAACAAAATAGAAGAAATA11.1904761904761905No Hit
GACGTGGACACTTGAAATCGCTAAATTTTGTGAAATATAAAATATACAAT11.1904761904761905No Hit
CCCTGGGATCATGACCTGAGCTGAAGGCAGCCACTTAATGACTGAGCCAC11.1904761904761905No Hit
CAAAGAATATGCGGAATATCCGAAAAACGATACTATCAATTTAGCTGAAA11.1904761904761905No Hit
CAACTACACATTCCTGGATTCACAATAGGAATCATTCAATTCACAACACA11.1904761904761905No Hit
CACCACCTCATGCCAGAACCAACCCGCTCAATAACAACACCATGGGAACA11.1904761904761905No Hit
TATCTTGGAGGTTCCATTGTTTCTCACATATAGGAACATGGGCCTCGACA11.1904761904761905No Hit
CAGATGGAGGACCAAACTTATACAATATACGGAATCATCACATTCCTGAA11.1904761904761905No Hit
CTCCCCACTGAGCAGGGAGCCCGATGCGGGGCTCGATCCCAGGACCCTGG11.1904761904761905No Hit
ATGCTGATGCATATGTTTTTGTGGGGTCATCAAGAAACAGCAAGAAGTTC11.1904761904761905No Hit
CATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTT11.1904761904761905No Hit
CAATTTGTACCAGCCAACTGGGGGGCGGCTGCTGCTGGATGGGAAGCCCA11.1904761904761905No Hit
AAACAAGGTAGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11.1904761904761905No Hit
CATTACATCCTGACCAGCACTTAAAACTCCATATTGTTAAATAATAGGCA11.1904761904761905No Hit
AATTGATGGCAATCCGAATTCTTTTGGTCGCTGTCTTGCTGTCAGTAAGT11.1904761904761905No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGAACCTGGCATCAATCCGG11.1904761904761905No Hit
CTGCTGGGCCACCCAACGCTCCCCAGCCCCATGTCCAGCTCCCGCTGCAA11.1904761904761905No Hit
GAAACAAATTTGGAGAATAAAAATTTACCTAGAAAGGAGAAATCACTAAG11.1904761904761905No Hit
TATTCAGACAATGCACATGCATCCTATCCAAGAACTGTAAGTCGAATGTT11.1904761904761905No Hit
GATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCC11.1904761904761905No Hit
CCGCATTTCCACGCGCCATTTCTGCGTGATGTATGAGGTTGCATTGGTTC11.1904761904761905No Hit
GTGTCATGTCTGTACTGCATTCTCACATCTGAAGACTGTTTTAGCTAGAG11.1904761904761905No Hit
TTATACTCGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA11.1904761904761905No Hit
GAGTGGTAGAGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG11.1904761904761905No Hit
CATCTAATAGATGGCACAGCATCACTGAGACCTCCGATGATGATGGGCAA11.1904761904761905No Hit
GTAATAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGATAT11.1904761904761905No Hit
CATATGAAATCACACTCTTTTTTTATAGCTATGGATTTGTGGATAATTTT11.1904761904761905No Hit
ACGCCAAACCATGAAAATTAGAGTCCAGGAAGGATATGAAGAATTCACCC11.1904761904761905No Hit
AGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTG11.1904761904761905No Hit
TAGTTAGCCAATTGATGCCATCATGACAAGCACTACCTCACCAAGCGACT11.1904761904761905No Hit
ACATCAACATTTCTCACTTCTCCTCCTGGAGTGAACATCTGCTCCCAGCA11.1904761904761905No Hit
ACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACA11.1904761904761905No Hit
CCATAAGACCATACTGTAACACCACTGACTGTCTAAACATTAGGGCGTGG11.1904761904761905No Hit
GTAGACTTTGATGAACAATTGAAGAGCCATCTGGGCCCTTGCAGGTCCAA11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTA50.0145.08
TGGTAAC50.0145.01
ACACTGT50.0145.06
CACTGTT50.0145.07
TAATTGA50.0145.0145
TAACACT50.0145.04
CTGTTAG50.0145.09
GTAACAC50.0145.03
AACACTG50.0145.05
GGTAACA50.0145.02
Produced by FastQC (version 0.11.5)