FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R2_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCTCTGTATATTTTCAGAGACTCGAACAGTGTTACCAATCCATTCAA11.2345679012345678No Hit
ATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTAC11.2345679012345678No Hit
CTATGGTTATGGCCAATGCAGATGCTGCCGTTTGAATTCTGTTTAATGAC11.2345679012345678No Hit
CATTAGGGAATACAAGCAGGAGAGGACAGATTCAACATGAACTGCAAGTT11.2345679012345678No Hit
CTAATTGATGGCAATACGAATTCTTTTGGACGCAGTCTGGCAGACAGTAA11.2345679012345678No Hit
GGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTG11.2345679012345678No Hit
AAACAAGCTCTTTTTTAAAAAATTCGACACTAATTGATGGCCATCCGAAT11.2345679012345678No Hit
GAATAAAAGAACTGAGAGATCTAATGTCTCAATCCCGCACTCGCCAGATA11.2345679012345678No Hit
CGAAAGGAATTACAAAATTACTTAAAAAGAAAAACAATCGTCCTACATCT11.2345679012345678No Hit
CATCCAAACCAACACCAAATAGCGATTTATCCCTTTCTCATGTGGTTACA11.2345679012345678No Hit
GTAGAACACATCTTCATTATAACAGATAACTCTTCTTTATTGATCCGACC11.2345679012345678No Hit
GTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCA11.2345679012345678No Hit
CAATAGTACGAACCCACTTTGATCTCTAACCCTTAAAAATCCGACAATAC11.2345679012345678No Hit
TCATTACCAACACTACGTCCCCTTGCACAATTAGCACAATAGCCTTCTCT11.2345679012345678No Hit
TCGTATTAAAAGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTT11.2345679012345678No Hit
CTACCAGGTTCACCCTAACTGCCTGAACAATGACAACCAAATTTCTAGAT11.2345679012345678No Hit
GGAGTCTAAGGGATACGGCCATGCTCAATGAATCCTGTGCATCCATGGAT11.2345679012345678No Hit
CATAATGAAGGGAGAGTACATAAATACGGCCTTGCTCAATGCATCCTGTG11.2345679012345678No Hit
TTTATGTAGTGGTGAACATGGAAGAAGGCACAGGGGTGCGTCTGCAATAA11.2345679012345678No Hit
CCTTCTGACCTTTACAATACACAAATCAAGTTAAAATAAGGAGGAGACTT11.2345679012345678No Hit
CAATAGTACGTTCCCTCTTTGATCACAAACCATTAAAAAACCTTCAAAAC11.2345679012345678No Hit
ATCCTAGAGTCCCCTTTTAGAATAAATACCAACACAACGTACCCATGCCC11.2345679012345678No Hit
AAATGGCAAGACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAG11.2345679012345678No Hit
GCACACTTTCCCAAAAGAGCCTTCCTCCACTCCCCTGGGAGACTCTCCGA11.2345679012345678No Hit
GTAATCTCACTGCAAGTAATAGACCATTTTCTTCATTCAAAGACAGGAGT11.2345679012345678No Hit
AAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAAT11.2345679012345678No Hit
GATCCAAAATGGAACACTTTGTGCGACAATGCATCAATCCAATGATCGTC11.2345679012345678No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGCAACAGGAAACA11.2345679012345678No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCAGGTTTCCTTAAAAAACC11.2345679012345678No Hit
TTTCAGACCAGAACTGCAGATAGGAAGATTCCCAATAGTACTCATTGCAT11.2345679012345678No Hit
ATTACCTCAACCTCGTGACTAGGGTCATAAATACTTAATTTCAGAGCAAC11.2345679012345678No Hit
AATCCAACCTCAGAACGCATGCATGACTTTATGGTGATCCCAATGATAAG11.2345679012345678No Hit
GTCCTAGCCAGCACCATTCTGATAACATGCCTGATTACAGGATTGGTCGT11.2345679012345678No Hit
ATCTTCATGAACTCAGCCAACTCATCGATCTTGGTCCGTCCAGACTCGAA11.2345679012345678No Hit
GCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGAGT11.2345679012345678No Hit
GAGGAAAAGGCAATGAAACAATATGGGGAACATCCGAAAATCGAAACTAA11.2345679012345678No Hit
AACCAAAGTCGATTTTAAACAATTCGACACTAATTTATGGCAATCCGAAA11.2345679012345678No Hit
AAGTGATACAGTAAAATATAATAGAATAAGCATTATTATCTCAATTTTCT11.2345679012345678No Hit
AAATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTCCTGCAATATGCA11.2345679012345678No Hit
GGATGTTACTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC11.2345679012345678No Hit
GAGTTGAACCAAGATGCATAAAGCAAAACCCAGGGATCATTAATCAGCCA11.2345679012345678No Hit
GGGGTAGAGAAGCCTAAAATTATTACTGATTTGTATGATTACAAAGAGAA11.2345679012345678No Hit
TATAGGAGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTTA11.2345679012345678No Hit
GTCTTCTCTCAAAGAGAAATACATGACCAAGGAATTCTTTGAAAACAAAT11.2345679012345678No Hit
ATTTACATGATCGACAACATAAATACAAAATAAAAGCCGACTGAGATCTA11.2345679012345678No Hit
GTCTATGGGATTACTTACGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA11.2345679012345678No Hit
CATATAGACTGTTGAATACAGATTTTGACAGTAAGATCCTGAACACTTTC11.2345679012345678No Hit
ATTCATCCCTCATGGACTCATGCACAACTACCGTAGAAGGACAACTATTC11.2345679012345678No Hit
GAATAATGACTTCGACAATAACAATTCACCAACAATCAGTTACGAGACAC11.2345679012345678No Hit
GTGCTATTCCCACCACCTCGACACTCGGGTATTCAATACTAAATTTCAGA11.2345679012345678No Hit
AAACAAACCTGTATGGGTTCAATATAAAAGGAAGGTCTCATTAGAGAAAA11.2345679012345678No Hit
GGTCAAATATAATCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.2345679012345678No Hit
GCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACAGACAAAG11.2345679012345678No Hit
ATCTTGCTCAACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATAT11.2345679012345678No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACATAATT11.2345679012345678No Hit
CAGTATGGATAGCAAATATTAGCATTGACACAAATACTTCAGTGCATGTG11.2345679012345678No Hit
CCTCCACAATTTCCCAATAGAGCAATACTCCACTCCCCTGGGAGACACTC11.2345679012345678No Hit
CGAGTGGACAGATTCTACAGGACCAGCAAGTAAGTGGGAATCAACACGAG11.2345679012345678No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATCCAACTGGCAAGTGCACCAGT11.2345679012345678No Hit
GAGGAAAGAAAGTGATTCCAAGAGGAAAGCTTTCCACAAGAGGGGTCCAG11.2345679012345678No Hit
GTCCCGCGCTCGCGACAGACTCACTAAGACCACTCTGGAACATATGGCCA11.2345679012345678No Hit
GTATCATTACAAAGAGACCCGGATCATGGAAATTCGAGTAACACGGAGGG11.2345679012345678No Hit
AGCAGGTACTGAACCAAAATCGAAGACTTTCTGCGACAATGCTTCAATCC11.2345679012345678No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG11.2345679012345678No Hit
GCTTCATATAGCCCCCCAAGATCCAACGTTCCAGGAACCAGCAAGTCCCT11.2345679012345678No Hit
GATGATGACTAATTCACAAGAAACAGAGATTACTATCACAATCACTGGCG11.2345679012345678No Hit
GAGACTGGTGCACCCCAGCTCAACACGATTGATGGACCACTACCTGAGGA11.2345679012345678No Hit
ATCAAATGCATGAAGACATTCTTTGGCAGGAAAGAGACTAACATAGTCAA11.2345679012345678No Hit
GATCCACCAAACAGCCATGGCACAGGAACAGCATACACCATGGACACAGT11.2345679012345678No Hit
CTATTGAAGAACCGATTTGATATAATAGAAGGAAGAGACCGAATCATGGC11.2345679012345678No Hit
CTCCTATAAGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT11.2345679012345678No Hit
GGAGGCAATTGAAGAAAAATTTGAGATTACAGGAACTATGCGCAAGCTTG11.2345679012345678No Hit
ACATTGTGGGATCTTGTGACCATTGAATTATCACAATTTCCCAGTACCTG11.2345679012345678No Hit
TAACGATGCCCCATAACAACATGATCTTGCAGGAACCATTGGTTCACACA11.2345679012345678No Hit
TACACATATAGGTGCCATAGGGGAGACACACAAAATCAGACGAGAAGATC11.2345679012345678No Hit
GGTCCAGTAGAATCTCTGCACTCCTGCTTGAATTCCATCATGCATTTGTG11.2345679012345678No Hit
GGCCCATGTTGGGATATGTGAGAGCCAATGGAACCTCCAAGAGCAAGATG11.2345679012345678No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCATGGCAATATATCTTGTCTTC11.2345679012345678No Hit
TCCGATTTGTTTTCAAACAATTCCTTCGTCATGTATTTCTCTTTGACAGA11.2345679012345678No Hit
GTCCACATATATTACATAGAGAAAGCAAACAAAATAAAATCAGAGAAGCA11.2345679012345678No Hit
GAAGAACCCCGCACTCAGAAAGAACTGGATGATGGCAATGAGATACCCAA11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATG50.0145.03
TTCATGA50.0145.04
CATGAAC50.0145.06
ATCTTCA50.0145.01
ATGAACT50.0145.07
TTCTCGC50.0145.0145
GAACTCA50.0145.09
TCATGAA50.0145.05
TGAACTC50.0145.08
TCTTCAT50.0145.02
Produced by FastQC (version 0.11.5)