FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGAGTACATGAAGGGTATGAAGAATTAACAATGGTTGGGATCAGA13.571428571428571No Hit
GCAGGCCGTTACAGGCAAAATCTGAATACAACAGTCTATATGCGTCTCCA13.571428571428571No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC13.571428571428571No Hit
AAGCACAACATTCACTTATAATGGAGATCCTACATACAGCCATGGAACAG13.571428571428571No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGTGCCCTAGACACCATGGCCTC13.571428571428571No Hit
GTACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA13.571428571428571No Hit
GAACAAGAGCTATTGTTCGCACCGGAATGTATCACAGAATGTTCTCTATA13.571428571428571No Hit
CACATATAGCAGACATATGGGAGAAACACAAATTCAGACAAGAACATCAT13.571428571428571No Hit
CACCATCGCCTCCCCCATGCTAGAAATTCCAACCGGTCTGCTATATGAAC13.571428571428571No Hit
GTCTGGATGTCAGTAAGTATGCTAGCGTACTGTTTTCTTTTAATTAACAA13.571428571428571No Hit
AGCAGCTAATTATCAAAAAAAGAAGACTTTGTGCGACAATGATTCAATCC13.571428571428571No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGAGAGATA13.571428571428571No Hit
GAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTAT13.571428571428571No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.571428571428571No Hit
GACTAATGCACCATACACAGCGATTGAGTTCCCCCTCCCTGTGGAACAAC13.571428571428571No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA13.571428571428571No Hit
CCTCAATGCAGCCGTTCGGCTCGAATCCATCAACATAGGCTCTAAAGTTT13.571428571428571No Hit
GGGGTTGTTCACGCCGGGGGGGGGTGGGGGAGCTGGGGGGGTGGGCCGAG13.571428571428571No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGGTTCATTTCCAACACTACGT13.571428571428571No Hit
CTTCGTCAACGGTGAAGTCCGCTTAGGTGGCCATCTCCACTCCAGTCAAT13.571428571428571No Hit
AACTACTTCAGGGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG13.571428571428571No Hit
ATCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC13.571428571428571No Hit
GCAATTAGCACAACAATCCCTAAAACTGGATAAACTCCATACAGCAATGG13.571428571428571No Hit
CTCCTAGCTAAATATTCAACAAAGTATACGAAACCTCTAATCTGCAACCC13.571428571428571No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT13.571428571428571No Hit
ATGCCACTCTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAG13.571428571428571No Hit
GTATGGAGCGGAAATAATAGCATGGGCAGACCTAATACAGGGCTTCTGTC13.571428571428571No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATGA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)