FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGGACTAAGCTGGGTTTTCACTCGCTCAAGACATCTGGTCAATAG10.6802721088435374No Hit
GATTCAAGCTGGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGAT10.6802721088435374No Hit
GAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAG10.6802721088435374No Hit
GTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAAC10.6802721088435374No Hit
ACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAGGAGAT10.6802721088435374No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG10.6802721088435374No Hit
TCCTTCTCCTCATCATCATGATCAAGCTGCCATGTTCTTGCTCTTCCTGA10.6802721088435374No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA10.6802721088435374No Hit
GCCCGTCTTCTCTCCGGAGCCTTCCCCAGGGGAAGCCCACCCTTGGGGGC10.6802721088435374No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA10.6802721088435374No Hit
GTATGCTAGAGTCCCGTTTTCGTTTGATTACCAACACTACGTCCCCTTGC10.6802721088435374No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT10.6802721088435374No Hit
AAACAAAGATTAATTATATTAAAAATACACCAATCTACCATACGATAATA10.6802721088435374No Hit
GTCTAATGCAAGGTTCAACACTTCCCAGATGGGCTGGTGCCGCAGGTGCT10.6802721088435374No Hit
GCGTTGTGGGGAGCTGTAGTCTGTGAGGGGGGAGTGGTGGGGGGTGTGGG10.6802721088435374No Hit
GCCTGGAAAACTTTAGAGCCTATGGAGATGGATTCGAGCCGAACGGCTGC10.6802721088435374No Hit
GGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAGCATTGTG10.6802721088435374No Hit
CCTTTAATGTTCTCTAACAAAATGGCAAGACTTGGGTAAGGGGACATGTT10.6802721088435374No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA10.6802721088435374No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA10.6802721088435374No Hit
TTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACC10.6802721088435374No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG10.6802721088435374No Hit
AGTATCTGCTGCTCAGTTCCAGGGTAGTGGAGTCCATGGTGTCCCCCTTC10.6802721088435374No Hit
CACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCC10.6802721088435374No Hit
GGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGA10.6802721088435374No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCACCACCATGGCAAGCTTTC10.6802721088435374No Hit
CTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGTATCAACTC10.6802721088435374No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT10.6802721088435374No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10.6802721088435374No Hit
AGATGCAACAGCAGGTCTTACTCATATCATGATTTGGCATTCCAACCTGA10.6802721088435374No Hit
TCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAG10.6802721088435374No Hit
CACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGA10.6802721088435374No Hit
GTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCA10.6802721088435374No Hit
GATTGAGGCCGAGTCTTCTGTCTTGGAGAGGGCCTGGACCGAGGATTTTT10.6802721088435374No Hit
GCTTACTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTG10.6802721088435374No Hit
GGCTCATCACTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATC10.6802721088435374No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG10.6802721088435374No Hit
GTGGCTGAGCAGAGGAGACACAGTGAGCAGTGCTGTGTGGAGGGACACCC10.6802721088435374No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG10.6802721088435374No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA10.6802721088435374No Hit
AATCCTGCCTCCCTCACAGAGCGTATGGGCAAGCAGAAGCATGAGCGAAT10.6802721088435374No Hit
GGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAG10.6802721088435374No Hit
AGTCAATTCCCATGCGTTAGTGAATTCATTTTGGGCCCAGCTTGCGAGAG10.6802721088435374No Hit
ATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTA10.6802721088435374No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC10.6802721088435374No Hit
TCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCT10.6802721088435374No Hit
CGTGTGGGGTGCGCGTCTGGGTTTAGGAGTATTTGTGCTAGGTGGGTGGG10.6802721088435374No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA10.6802721088435374No Hit
CTCTCATGCTCTCTCTCAAATAAATACAATCTTAAAAGAAAAAAAAAAAA10.6802721088435374No Hit
GGGCCCCACAGGCCCCCCCACCCTACTTTTAGCAACACATCCTCAAATAG10.6802721088435374No Hit
CCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTTC10.6802721088435374No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT10.6802721088435374No Hit
CATCACGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACAGGATT10.6802721088435374No Hit
CCACAGGTGAGCCTTGAGGGTATTCAGCCAAGTAAGATATGCCAGGTCCG10.6802721088435374No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10.6802721088435374No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC10.6802721088435374No Hit
CTTTTTAGTCATAGAATATGCACTTGCAAACGCTACTGAAAAAACCCAAG10.6802721088435374No Hit
CTACTAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA10.6802721088435374No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATA10.6802721088435374No Hit
CCCTGGAACCAGACTTTCCCCACTTGCTTAGGATGAGAAACGCCTTGGGG10.6802721088435374No Hit
ATCTGGGCCGTGGCGGGTCCAGGGTCATTGTGGATCTGGTTGGGCGTTAT10.6802721088435374No Hit
GATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCT10.6802721088435374No Hit
GTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACACATGCACATTCA10.6802721088435374No Hit
TGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTG10.6802721088435374No Hit
GCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGG10.6802721088435374No Hit
ATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTT10.6802721088435374No Hit
GGGGGGGGGCGGCGGCGGGGGGGGCGCGGGGGGGGGTTGTGGGGGGGGGG10.6802721088435374No Hit
TGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCA10.6802721088435374No Hit
CAAAATATTGATACCCCTGGTGGGGACGCTACCCCCACACAAAGCATTGA10.6802721088435374No Hit
ACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTCACATC10.6802721088435374No Hit
TCACACATGCTTCTCTAGCAGAGGGAACAGATGAATGGCTTCCCAGGAAT10.6802721088435374No Hit
TCTCTTCATTTTCAATGTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCC10.6802721088435374No Hit
CATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAATTTT10.6802721088435374No Hit
TTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGAGAAAAGCTTG10.6802721088435374No Hit
GGCCACGTCAGAGGGCGCCTGTGAAATTTCCATGTGAACAACCCACAGAT10.6802721088435374No Hit
GAATTCTATTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCACGT10.6802721088435374No Hit
GGATACACCATGGACACACCAATACTCAGAAAAGGGAAAGTGGACGACAA10.6802721088435374No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG10.6802721088435374No Hit
GAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAA10.6802721088435374No Hit
ATATAATGGTATTCCCTCCCCCTCGTGCCTCGGGTCTTCAATACTTAATT10.6802721088435374No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT10.6802721088435374No Hit
GTCTTTGAAAATATTTTTTGTTCAGTCACCAACATCAAGCTGCCTATATT10.6802721088435374No Hit
AGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAG10.6802721088435374No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA10.6802721088435374No Hit
GGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAG10.6802721088435374No Hit
CTGCGGGCTAGCACAGGAGGGAAGGATGAAGGGCGCCTGGGGTGTATGAT10.6802721088435374No Hit
GGCTATAACTACTGCAAATGCTATGAAACATATTTCTGGATCTAGTTAAC10.6802721088435374No Hit
GCTCATGGACAAGGCCCTGCTCTGCAGCGTGTGCAAGATGACCTGCCACA10.6802721088435374No Hit
CCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTC10.6802721088435374No Hit
ATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGAC10.6802721088435374No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAG10.6802721088435374No Hit
CTCTTTGATCTCTAACCCTTAAAAATCGGTCAATACTCACTACCACTCTC10.6802721088435374No Hit
ATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATACCTTTCGTCAGTC10.6802721088435374No Hit
GGTCAAATATATTCAATATGGAGAGCATACAAGAACTGAGAGATCTAATG10.6802721088435374No Hit
ACCACAAACAAACAAAACGATTAACGCAAAAAAAACAAATACCACATAAA10.6802721088435374No Hit
AACTTATACATCAAAACGCTAGAACCAGAGAAAAGCTGTATGTTGGCCTG10.6802721088435374No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA10.6802721088435374No Hit
GTAGTAAGAGAAGAAAAGGAAGCAGAACAAAGCAGGAGGGCCCTTTTCTC10.6802721088435374No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10.6802721088435374No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA10.6802721088435374No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC10.6802721088435374No Hit
GCTCGGGGTGGGTGGTGGCGGTGGCGGGGGGGTGGGGGGGTGGGTGTGGG10.6802721088435374No Hit
GTCCCAAGGCCACCCGGCAGCTGGGCTCTGGCGTCTTCTTGCTTTGTCCC10.6802721088435374No Hit
AGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAG10.6802721088435374No Hit
TAATGGATCTTTGTTTGAAAGGGTGGGGCCCCCCGGGCGGGGGGTGGTGT10.6802721088435374No Hit
GGGGGGGGGGGGGGGCGCGGGGGGGGGGGGGGGGGGGGGGGCGGGGCGGG10.6802721088435374No Hit
AATTCGTCCGTTTCTTGCATCTTCAGATCCGCATGGAGCTCGGCCAGCGT10.6802721088435374No Hit
GCTTCATAGCGAAGTTATTCACAACAGACAAAAGGTGGAAGCAACCCAAA10.6802721088435374No Hit
CTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGGAATTGGGATTTA10.6802721088435374No Hit
CAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCAT10.6802721088435374No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA10.6802721088435374No Hit
GTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTG10.6802721088435374No Hit
GGGGGGGGGGGGCTGTGGCGTTGGTGGTGGCGGCCGGCCGGGGGGGGGTT10.6802721088435374No Hit
AATTGACAATTAATCCCTTGAAACAAAGGAAGTTCTACATCAATCAACGG10.6802721088435374No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10.6802721088435374No Hit
TCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGCCTCGA10.6802721088435374No Hit
AGACATTCTACTGGTTGGAGGGGCTCCCATGGTCCGGCGAGTGAGCTGTC10.6802721088435374No Hit
GGATTGGGTGATGCCCCATTCCATGATCGGCTCCGCCGAGATCAAAAGTC10.6802721088435374No Hit
TATTTAATGTCAGTGCGCTTATTAGATAGCCTCTCTTATTCAGTCGTTGT10.6802721088435374No Hit
TTATATACCTTAGGGTAATGAACTGTACTTGTTGCTGTCTCTTATACACA10.6802721088435374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.6802721088435374No Hit
GTGCCATAGTGAGTTGTACTAGTTTCATATCTATTTTCCTTTTACTGTAT10.6802721088435374No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT10.6802721088435374No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT10.6802721088435374No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA10.6802721088435374No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA10.6802721088435374No Hit
CACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGG10.6802721088435374No Hit
GCTTTTGATGAGAGAAGAAATAAATACCTAGAAGAGCATCCCAGTGCTGG10.6802721088435374No Hit
CTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGT10.6802721088435374No Hit
GATACTACTAAGGTATTTAACTGAGGAGGGAGCAATATTTGGAGAAATTT10.6802721088435374No Hit
GGCTTATGGTGGAGTGGAGAGCCAGAGGCTGCTGCCTGGGGTGAAGGCTC10.6802721088435374No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.6802721088435374No Hit
GGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCA10.6802721088435374No Hit
AGTAAGAGAAAGTCGAACCCCAGGAACCGCTGAGATTGAAGACCTCAGGT10.6802721088435374No Hit
AATTGAGATTGTGGAGGCCATGGGCTGCAGTGCCCTGGAGGTGCGGGGCG10.6802721088435374No Hit
TGTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGG10.6802721088435374No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.6802721088435374No Hit
TTATCTTTTCATTCTTAATGAATTATATGAACCAATCTGGATCATTACTG10.6802721088435374No Hit
CCTAAGTGGTGCCTAGTTGTTCTGTATGACTCTCACCTGGCCTTACCTCC10.6802721088435374No Hit
CGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGG10.6802721088435374No Hit
GGCCTGTAGCGCGGCTAGCGCCTGCTTGCACGCCGGGCGGTTATTGGGGT10.6802721088435374No Hit
GGCCCCAAATAGGCCTCTAGATTGAATAGACGGGATATTCCTCAATCCTG10.6802721088435374No Hit
CATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGACTACACCC10.6802721088435374No Hit
GATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAG10.6802721088435374No Hit
GGACCTCTGGCGTGCAAAGCACGTGTTGGAAAAGTGAGAGACAGTGGTGA10.6802721088435374No Hit
ATACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTT10.6802721088435374No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.6802721088435374No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers