FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCATGTGAAATGCCTGATTCAAGCTGTATAGAACTTGATGAAA12.564102564102564No Hit
GAAAAATAGTAGAAAAAAGGCATAATATCATCAACAAAAAATAAAATAAA12.564102564102564No Hit
GCAATTGCCTGCTCGTCTCTCCCGCTTACTATCAACTTGCGCTATCTCCT12.564102564102564No Hit
TGGCAAACCAGCGCCTGCACCCCATGCGCCAACTCTTGAGGCATTTCCAA12.564102564102564No Hit
TCCAATCTGTGTTCTGTGGCACATTTCCAAGAGAGATCCTAATGGGTCTG12.564102564102564No Hit
TTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAA12.564102564102564No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12.564102564102564No Hit
CCCTTGCGTAAACTGCCACACGGCAATATAAACAAAGCCTGTACCGCCGG12.564102564102564No Hit
CAAAAGAAGTGAACGCAAAAATTGAACCATTCTTGAGGACGACACCACGC12.564102564102564No Hit
GTTTCAGAAGACCAATTAGCATCTCTCATGGAAATGTTACTCAGAACACA12.564102564102564No Hit
CCCTTATACTGTAAATCCTCAATAAATCAATGGTACAGTAAAAGGATACA12.564102564102564No Hit
GTATTACTCTCCGACACTTTTGCTGCCTCTGAGAACTCTAAGCGACAGCT12.564102564102564No Hit
AGTCAACACTTATCAATGGATAATCTGGAACTGGGAAATTGTGAAAATTC12.564102564102564No Hit
CTACCTGACGATAATGAACAAAGTGGGTATGCACAAACAGACTGTGATAA12.564102564102564No Hit
AAAGATAAGCGAAAATACTCAATTAATAAATTAAAACAATGGAAACAAAC12.564102564102564No Hit
CAGTAGCGCCATCCTCAACTCCCCTGGGTGACTCTCGGATTGGCCATGTT12.564102564102564No Hit
TATAGGAACATCCGCCACGACAAATGGCATAACGAATTCATCAACAGCAG12.564102564102564No Hit
GTTTGAGGTGGGGCTGGAAACCTTTACGAAGTCTTTGGAAAAATATGATA12.564102564102564No Hit
ACCTTGGCCTCGATATCTAAACAGCCACTCTTGTTGGGAAACAAATCGTG12.564102564102564No Hit
CAACTATATGAAGCAATCAAATGCATCAAGACATTCTTTGTCTCGAAAGA12.564102564102564No Hit
GACTACACCCTTGACGAAGAGAGCAGTGCAAGAATCAAAACTAGGCTGTT12.564102564102564No Hit
TTCAGTCGCCCTTCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCA12.564102564102564No Hit
GCTAGGAAGCATTTGCAAGCAGAGCTACAGGAGATTGAAAATGAAGAGAA12.564102564102564No Hit
CCGATATTTTAGCAACAATGGAACATCCAATATCAAGAGGAAATGGGGAA12.564102564102564No Hit
CTCTTGAGGCGTTTCCCACAAGATGCAAATGTGCGTTTCAAGAACTGGGG12.564102564102564No Hit
CAGTACCAACAAAGCGCAACCTTTAAGAAATGACCCTCCATGAAATGAAA12.564102564102564No Hit
GCTACTGGGAGAAACCTTGTTTTACGGCCCAATTCTCTTTCTAGCATGTA12.564102564102564No Hit
GTGGTATTCCCTCCACCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA12.564102564102564No Hit
ACACTTGTCATTCTTACATTTTCACCACTACAGAAATTTCTTTCATTTAT12.564102564102564No Hit
GTTCAAGCTCATGCCTAGGCAAAATATAATAGGCCATCTTTGCGTGCGAT12.564102564102564No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGAAGAGAGAG12.564102564102564No Hit
ACAAGGTCTTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTC12.564102564102564No Hit
GGAGCTCGGATGAGTCCCAATAGGTCTCATTGCATGGCCCATCTGCCTCG12.564102564102564No Hit
GAAAAAAGTCATATGCTTTTAAAAAAACTTGTTTATAGGGATGCCAGGGT12.564102564102564No Hit
CGCGCAGAGACTCCAAAGTGTCTTTCCAGGAAAAAACACAGATCTTGAGG12.564102564102564No Hit
GGGGAAGTTCGGTGGCAGACTTTGGTCGGCAAGCTTGCGCATAGTTACTG12.564102564102564No Hit
ATATAGTACTTCTTTTTTCTAATGTTGATTCCAACTAAATTTAAGGTAAT12.564102564102564No Hit
ATTTAAGATCCAGATGGGCCGTGGCATGTCCAAGGTGAATGTTAATCATG12.564102564102564No Hit
GCAATGCAGAGGGTCACTTCTGATTTAAAATAGATCAAACTATTTATTGC12.564102564102564No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)