FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT40.3755868544600939No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC30.28169014084507044No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.28169014084507044No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA30.28169014084507044No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.28169014084507044No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.28169014084507044No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT30.28169014084507044No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG30.28169014084507044No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG20.18779342723004694No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA20.18779342723004694No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA20.18779342723004694No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.18779342723004694No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.18779342723004694No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG20.18779342723004694No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG20.18779342723004694No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.18779342723004694No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT20.18779342723004694No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC20.18779342723004694No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT20.18779342723004694No Hit
CAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGC20.18779342723004694No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG20.18779342723004694No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT20.18779342723004694No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTA20.18779342723004694No Hit
CTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCA20.18779342723004694No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.18779342723004694No Hit
GATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCA20.18779342723004694No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT20.18779342723004694No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG20.18779342723004694No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)