FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGAGTCCTACGATATCTGGCTGTATGGCGAAGTTCTTTTCTGTCCCA15.263157894736842No Hit
CTCCAGCACGGAGAGAGTGGGAGTGAGTATTGACCGATTTTTAAGGGTTA15.263157894736842No Hit
CTCGAATAGCTGGCAGAACTTCTGGTACATCTGTTAATCCTAAAGAATTC15.263157894736842No Hit
TAACCCAATGGCCGTGATGGGAGCAGATGTACACTCCAGTAGGAGAAGGG15.263157894736842No Hit
AGTAGCAACAAGGTACGTTTTTGGACAGTATGGATAGCAAATCGTAGCAT15.263157894736842No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG15.263157894736842No Hit
CTTGTGCGCTGTCTTTTCATTTGGTCTAATCAGTCTGAACAATTTGCTTT15.263157894736842No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT15.263157894736842No Hit
AACGTACTTAGCATGATCAACATTCACATCATCATCCCAGGACTCAGTGA15.263157894736842No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCACCCCTGCTCCTCTCACATTC15.263157894736842No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT15.263157894736842No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC15.263157894736842No Hit
GTCAACGGAATCAATCAAGTTAACAGAAGGATGAGAGGTGCGACACACCA15.263157894736842No Hit
TCACGAACGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGTC15.263157894736842No Hit
CTGGATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGA15.263157894736842No Hit
GCCTCTCTCTGCATCTTTGCTCATCGTATTTAATGTCAGTGCTCTTATTA15.263157894736842No Hit
GTATAGGTTATCAGGCGAACAATTCAACAGACACTTTAGCCACAGTACTA15.263157894736842No Hit
TACCAGGACTCAGTGATGCTGGGCCAGCTATTAGAAGAGGCCGGATTTTC15.263157894736842No Hit
GAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)