FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_C06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_C06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.2380952380952381No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40.2380952380952381No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.17857142857142858No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT30.17857142857142858No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG30.17857142857142858No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT30.17857142857142858No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.17857142857142858No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.17857142857142858No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.17857142857142858No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.17857142857142858No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA30.17857142857142858No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.17857142857142858No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.17857142857142858No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT20.11904761904761905No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20.11904761904761905No Hit
GAGTACAGATGTAGGGGAGAAAGGGAAGCAGCTGGCTGCGGGACAGAGGC20.11904761904761905No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT20.11904761904761905No Hit
AGTTCCGTGAGAAGTGTTATTGGTATTTTGATAGGTATTACATCAAATAT20.11904761904761905No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC20.11904761904761905No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG20.11904761904761905No Hit
GTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCA20.11904761904761905No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA20.11904761904761905No Hit
AAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCAC20.11904761904761905No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA20.11904761904761905No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA20.11904761904761905No Hit
CCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCT20.11904761904761905No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.11904761904761905No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20.11904761904761905No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20.11904761904761905No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA20.11904761904761905No Hit
GTCCCTCACAGAACCCGAGAAGAGCACAGGCTCCTGCTCCACCGAAGTCA20.11904761904761905No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.11904761904761905No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20.11904761904761905No Hit
GGGGATGGCAAGTACCTAGGAATAAACATAACCAAAGAATTAAAAAATCT20.11904761904761905No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA20.11904761904761905No Hit
CTTCATCAAGGGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAAT20.11904761904761905No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG20.11904761904761905No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.11904761904761905No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.11904761904761905No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA20.11904761904761905No Hit
GGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCAC20.11904761904761905No Hit
GGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATT20.11904761904761905No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.11904761904761905No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.11904761904761905No Hit
GTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGCTGC20.11904761904761905No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG20.11904761904761905No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)