FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCC10.9803921568627451No Hit
ATACTGAATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGGGA10.9803921568627451No Hit
AGTAGAAACAAGGCATTTTTTTCATGAAGGACAAGTTAAATTCATTATTT10.9803921568627451No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT10.9803921568627451No Hit
GAATTGAGGATTGGTGGTAGAAGCCATCTGTCTGTCAGACCGATGCTGTC10.9803921568627451No Hit
CACTAAGACCACTGTGGCCAATATGGCCATAATCAAAAAGTACACATCAG10.9803921568627451No Hit
GGGGGGGGGGGGGGGTGGTGCGGGGGGGGGGGCGGGGGGGGGGGGCGGGG10.9803921568627451No Hit
ATTACTTCCTGATTTGAATGATTACAAAGAGAACCGGTTCAATGAAATTG10.9803921568627451No Hit
GGTTACACCATGGACACAGTAATCAGAACACACCAATACTCAGATAAGGG10.9803921568627451No Hit
CCTTTATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.9803921568627451No Hit
GAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGAT10.9803921568627451No Hit
ATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACT10.9803921568627451No Hit
CCCCACATACAGGCCTCTCCGCCAATCACTAGAGCCTTTTGCTGAGGGCT10.9803921568627451No Hit
GCGTGGGGGTCGGGTGGCGGGGAGGGGTGGGGGGTCTGAAGTGTCATAAG10.9803921568627451No Hit
AAATAGCACTTTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA10.9803921568627451No Hit
ACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGC10.9803921568627451No Hit
CTGGGGGAACCGTGGGGTGGTATGCCAGGCGAGGGTGGCACGGGCCGGAG10.9803921568627451No Hit
ATATGACGCAACCTATGGGAGCTCCAAGTACTAGTTGAAATGGGGCTTGG10.9803921568627451No Hit
CATCAGTATCATTTCTCAAATGCGACCTTCCTTTTATAATGAACCCATAC10.9803921568627451No Hit
TAGTTAAGATGCAGCATCTGTCAATGGGCCGGCAGCCATTAGAATCTCTG10.9803921568627451No Hit
CCTCATACAACAGGAAGGAGAGCAGCCCGCCTTGAGTGAGGTCCCCAGCC10.9803921568627451No Hit
AGGGGTGGGAGCGGCCGCGGGGGGGCGTGTGGCGGGGGGGGGTGGTGCTG10.9803921568627451No Hit
GAGAGATCTAATGTCGCAGTCCCGCGCTCGCGAGATACTCCCTAAGCCCA10.9803921568627451No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC10.9803921568627451No Hit
TGTTCCCTGGATTTGGTAGTACATGGGACCAAATCCTTTAGGTCCTAGCA10.9803921568627451No Hit
CAGTAAGTATGCGAGCGTCCCGGGTTCGGTTGCTTGCCCACCCGACGGCC10.9803921568627451No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC10.9803921568627451No Hit
ATGTGGATCTTGGACACCGCCAGGTCGGCCCTGTCGGGGTGCGCCGGTCA10.9803921568627451No Hit
GGATGGCGGCATTCAGGTTACTCCGCACCCGCCAGGTGGTCAGCCTGATG10.9803921568627451No Hit
CCATCCCAGGACCCTAGGATCATGACCTGGGTGGAAGGCAGAGCTTTAAC10.9803921568627451No Hit
TAGTAGCATTGGCACTCGTTGTTCTGTGCAGGTGTGAGGTAGGAGTTGAA10.9803921568627451No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA10.9803921568627451No Hit
TAACAAAGGCTATATAATAATAAAACAAACAAAAATAACGATAAAAAAAA10.9803921568627451No Hit
CTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGC10.9803921568627451No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT10.9803921568627451No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT10.9803921568627451No Hit
CATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATG10.9803921568627451No Hit
ATTGAAGCGCCATCTGGGCCGTTGCAGGTCCATGGTGTTTGTTTGTGATG10.9803921568627451No Hit
TCTTAAGCCTTCAGAACATTCCATCTTGACTTAAAATTCTGAATGCTTGG10.9803921568627451No Hit
GGGTGATGAGGGGCCAGTGGGGCGCGGGGGGCCGGCGGGGGCTGCCTTGG10.9803921568627451No Hit
ATCCCAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGAC10.9803921568627451No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.9803921568627451No Hit
AAGTCAGTATTCTAGCCCACCGTTTTCTTTTTTTTACGAACACAGCGTCT10.9803921568627451No Hit
CCAGAATGTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGT10.9803921568627451No Hit
GGCGGGGGGGGGGGGGGGGGGCGGGTGGGGGGGGGGTGGGGGGGGGGGGA10.9803921568627451No Hit
GTATTGGAGTAACAGTGATATGGGACACCATGTTTAACGATGGCGTTGGG10.9803921568627451No Hit
GACCTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA10.9803921568627451No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC10.9803921568627451No Hit
ACACTAGCCTTCTCTCCTTTTGCAAGATGGCTCAGTTCATTGATGCATAA10.9803921568627451No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTC10.9803921568627451No Hit
ATGCGGGGCTCTACTGGGGCAGAGTGGACGCGTGGCAGCTGCATGGCAAC10.9803921568627451No Hit
GAAATCCTCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGA10.9803921568627451No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAACAATCCCACCCAGGAATATTT10.9803921568627451No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC10.9803921568627451No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10.9803921568627451No Hit
CGCGAAGGGCTCATTCCCGCTCTAGCGAGCTACTCCCTTCTACCGCTGTG10.9803921568627451No Hit
GTGTGGGGGGGGCCGTGGGCGGGTTGTGGTGGGTGGGCGCGTGCGGGGGG10.9803921568627451No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.9803921568627451No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG10.9803921568627451No Hit
GTTTTCAAAGAATTCCTTGGTCATGTCGTTCTCTTTGACAGAAGACTCGG10.9803921568627451No Hit
CCCCTACATCTGTACTCAGTCCGTGCTCCACCTCCTGTATGAAGCCATGG10.9803921568627451No Hit
GTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGA10.9803921568627451No Hit
AGGATATGCAGCCGACCTCAAGAGCACACAGAATGCCATTGACGAGATTA10.9803921568627451No Hit
CCCAGGTGCTGGGCCAGGAGTCTCGATTCCCACAGGACACCCGCCATGCT10.9803921568627451No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC10.9803921568627451No Hit
ATATATCTTAGTTTAGCAACTTAGGATTTCAAATGCCTGATTTTTGAGGG10.9803921568627451No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATAGTC10.9803921568627451No Hit
CCATCCCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.9803921568627451No Hit
GTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTC10.9803921568627451No Hit
GTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGG10.9803921568627451No Hit
CCGTGGAGAAGGACCTGCATGGTGGGGGGGAGATTGGGAAGGGAGACACG10.9803921568627451No Hit
ATGGTATCTTTTCTGGTTTTACTATTCAGTCTCAAGAACCCATCCACATG10.9803921568627451No Hit
GGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCA10.9803921568627451No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA10.9803921568627451No Hit
CCGTTAGAATGAAAAGTGCAAATGTGCCTAGTTCGCGCTACATTTCTGGC10.9803921568627451No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCGTCCGAAT10.9803921568627451No Hit
GGTCAAGGAACCAAAGGAGGGATAAGGGCCAAGGATCTGGGCAGTTGGAC10.9803921568627451No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.9803921568627451No Hit
GTGCGGTGGGGGGGGCGGGAGGGGGGCGTGGGCGGGGTCTGCGGGGGGGG10.9803921568627451No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.9803921568627451No Hit
GTGCTCAAGGGGCTGCCGTTCCCTCTACGCCCTGGTCAGAGGACGGCTCT10.9803921568627451No Hit
CACCACCGGGTCCGCGTCAATTTAAACACACAGGGACGATTCCGTCTTTG10.9803921568627451No Hit
CAATTATTGCCTCAGCCATTGACTGCTCCTCTAGCCCGCCTACGATCAAC10.9803921568627451No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG10.9803921568627451No Hit
GTTCTTTGATGCTTTATGCTGGGCCTTATCTCTTGTGTTGTTTGACCATA10.9803921568627451No Hit
CCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTT10.9803921568627451No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA10.9803921568627451No Hit
CTCAAGAATGGTTCAATTTTGGCGTTAACTTCTTTTGACATTTGGGAAAG10.9803921568627451No Hit
GACTAGCCCTGACTCAGGGCCCTTGATCTCCCACATCAGGGATGCCGAAT10.9803921568627451No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10.9803921568627451No Hit
AATCCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATA10.9803921568627451No Hit
GCCCTCCAGAGGCGCCCTACTCACTCGCTTTCAGGTTTGTCCAGCTAGCC10.9803921568627451No Hit
CTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGGA10.9803921568627451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG10.9803921568627451No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT10.9803921568627451No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG10.9803921568627451No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10.9803921568627451No Hit
CACCACAACTGAAAGATGAGGTAATCCTCAACCCTATTGGTGCCTTGCAT10.9803921568627451No Hit
CTGTCTTTCGCTCAGTATGATAAGATCTCCAGAAGGAGTGCTCGATCTTG10.9803921568627451No Hit
GAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAG10.9803921568627451No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG10.9803921568627451No Hit
AAAAAACAACGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATT10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)