FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_C03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_C03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGGGTTTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGT13.7037037037037033No Hit
ATACCTACAAATTGGATGGCTTAGAGGAACAGAAATGTATCCTCTCCCTG13.7037037037037033No Hit
AAATTCGCGGCTAATTGCTGGCCATCCGAGTTGTTTTGGTCGCTGTCTGG13.7037037037037033No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13.7037037037037033No Hit
CATAAAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT13.7037037037037033No Hit
AATATATTCAATTTGGAGAGAATAAATGAACGGAGAGCTGTAATGTCGCA13.7037037037037033No Hit
GTTTTCGTTTCTTTACCAACACTACGTCCCCTTGCCCAATTAGCACATTA13.7037037037037033No Hit
CTCCACGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA13.7037037037037033No Hit
ATTCTACACTGTAGAGACCCATTAGAGAACAGCCAGAAACTGATTGACCC13.7037037037037033No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTATAATTTTCCTAAAAATT13.7037037037037033No Hit
ACATTATTTGGATGGAAAGAGAATAACATATTCAAACCACATGAGAAAGG13.7037037037037033No Hit
TGAAAACCCAGCTCACAAGAGTCAATTGTTGAGGATTGCATGCCACTATG13.7037037037037033No Hit
GAACAATATAATCACAAATTACTCATCCAATACCTCTCTCCAGTGATCTT13.7037037037037033No Hit
GGAGACTATGAAATTAAGAATATAAGACACGATTCACGACTCAGAGTGAA13.7037037037037033No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGCAGAACGCCGCACTCAGAAT13.7037037037037033No Hit
TTCTTTATCCGTCAAGACTAGAAGTCGACCCTGGCAACAATACGGTACCT13.7037037037037033No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGAGATAATCTCGCAGTCCCGCA13.7037037037037033No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATACGAATTCTTTTGGTCG13.7037037037037033No Hit
AACTTTAGCTAGGAGCTTTTGCGAAAAGCTTGAACATTCTGCGCTCCCAG13.7037037037037033No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC13.7037037037037033No Hit
TTTCTGTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATC13.7037037037037033No Hit
CAGAATGAAGATGATGATGGCAATGACATACCCAATTACAGCAGACAAGA13.7037037037037033No Hit
CATAACCACAGCATTGTTTACAAAATCAAACACTTTATGCCAGACAAAGG13.7037037037037033No Hit
CTATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGT13.7037037037037033No Hit
TATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGC13.7037037037037033No Hit
CATCTGTACCTACTTCGCGCTACCTTTATGACATGACCCTCGAGGAAATG13.7037037037037033No Hit
ATACTGGATAAAAACCATACATCCATGCACCTGTAACAGTATCAACCAGG13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)