FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAATGTGATCAGGCACTCCACGACTGGAAATCCCACCTCCACCCAA12.380952380952381No Hit
GTTTGATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC12.380952380952381No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA12.380952380952381No Hit
TTTCTCATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCT12.380952380952381No Hit
GCCACAATTATTGCCTCAGCAATTGACTGCTCGTCGCTCCCGCTGACTAT12.380952380952381No Hit
TGCAGAGCCTGCTCACACTGGACGTCCAGAGCACTGGTGGCTTCATCCAG12.380952380952381No Hit
GGGTTCAGTCGCTTGTTTGAACTATTGACAAAGTTAAGATAGCCCCTAAC12.380952380952381No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC12.380952380952381No Hit
GTATAAGTTTGGTCATCCATCTGATACTAATAGCCCTACCTTTGATTGGG12.380952380952381No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA12.380952380952381No Hit
CAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATG12.380952380952381No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12.380952380952381No Hit
GGGGGGGGGGGGGGTGGCGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG12.380952380952381No Hit
AGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCCGAACGGCTG12.380952380952381No Hit
TCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTCCT12.380952380952381No Hit
ACGCGAACGCTTTTTTCTTTTTACGTCAAGCCTCGAAGAAGACACAGTCA12.380952380952381No Hit
ATAATTTATGTAAATAAAAATTATTATCGTCTATGTCTGGCTGTAAGTAA12.380952380952381No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC12.380952380952381No Hit
CCCACATCATTGATTACGAATAAGTTATTGTAAAATTATAAGTTAATTTC12.380952380952381No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT12.380952380952381No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12.380952380952381No Hit
ATCCGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCAA12.380952380952381No Hit
GAAGAGGCCCCAATCTTGGAATAGATATCGAACACGACACGCTTGATGGT12.380952380952381No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12.380952380952381No Hit
GTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGA12.380952380952381No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGCGGACTTCTTTCCC12.380952380952381No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC12.380952380952381No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGACTCCGCCGAGATCAAAAGTC12.380952380952381No Hit
GGTAAATCCTTGTTGATTCCAGCTTTACCCCATCTATTTCTTCTCTGTTT12.380952380952381No Hit
GGAAATAGCAATAAGACCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT12.380952380952381No Hit
GTCTGGGCTCCCAGGAGGGGGCAAGGAAAAGAAGGCCACACGGGCACATG12.380952380952381No Hit
ATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATA12.380952380952381No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCTAGAGCGATGCGAATGGGTC12.380952380952381No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT12.380952380952381No Hit
ATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGAT12.380952380952381No Hit
GTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAAAAATACT12.380952380952381No Hit
AGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAG12.380952380952381No Hit
TCCCAAGCCACCCTGTGGTTCTCTGGAAGTGGCCCCAATCACTGGGGATG12.380952380952381No Hit
ATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATC12.380952380952381No Hit
AGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGTCATTGC12.380952380952381No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA12.380952380952381No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)