FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG100.34746351633078526No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT50.17373175816539263No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.13898540653231412No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA40.13898540653231412No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.10423905489923557No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.10423905489923557No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA30.10423905489923557No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATACTGGGAAGGGAGACACG30.10423905489923557No Hit
ATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCT30.10423905489923557No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.10423905489923557No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG30.10423905489923557No Hit
GTATGGTTTTGAAGCCATCCGTCCAGGAACGGCGACGGGCCAGAGAATTT30.10423905489923557No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA30.10423905489923557No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA30.10423905489923557No Hit
GGAGTGAGGCCCACTGGGGTCAAGGAACCAAAGGAGGGATAAGGGCCAAG30.10423905489923557No Hit
CCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTG30.10423905489923557No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG30.10423905489923557No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC30.10423905489923557No Hit
CTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTC30.10423905489923557No Hit
GGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGG30.10423905489923557No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30.10423905489923557No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG30.10423905489923557No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)