FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R2_B10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_B10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT23.7037037037037033No Hit
CTATTAGAAGAGGCCTTAATTTCTCAATTTTCTTCTTTGTTGATTCATTG11.8518518518518516No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG11.8518518518518516No Hit
CCTCCAAGATAAAGATGAAATGGGGCATGGAAATGAGGCGCAGCCATCTT11.8518518518518516No Hit
AGGACCAGCCAATACCATGGAATAAGATGCAGTTGGAACAACACATTCCT11.8518518518518516No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC11.8518518518518516No Hit
CTAATAGATGGCCATCCGAAATCTTTTCGTCGCTGTCTGGCAGTCAGTAA11.8518518518518516No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA11.8518518518518516No Hit
GAAAGGATCTCAATCCGACTCTACTTATCATAAAAATTCAAGCTCAAAAA11.8518518518518516No Hit
GCCACAACTACTTCAGTCCATCAGTGAGGAAGCACATGAACAAAGATCCA11.8518518518518516No Hit
TCCCAATTCTAAGAAAATGTAGGACCAAAGAAGGAAGAGGGAATACAACA11.8518518518518516No Hit
CCATGCGGGAATACAAGCATGAGTGGACAGATACAACAGGACCACCAAGT11.8518518518518516No Hit
GGAACAGTCACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGAGA11.8518518518518516No Hit
CTCTAAAGCAAGGTTCCACACTTCCCAGAAGGTCTGGAGACGCAGGTGCT11.8518518518518516No Hit
CAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTC11.8518518518518516No Hit
GTATGTTTCATGTATTCGGATTTCCATTTCTGCGACGCCCGGGCTCAATC11.8518518518518516No Hit
CTATGCGCAAGCTTGCCGACCAAAGTCTCCCAACGAACTTCCCCAGCCTT11.8518518518518516No Hit
CGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGATC11.8518518518518516No Hit
ACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGT11.8518518518518516No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACAACTTCAGTGCATGTGTG11.8518518518518516No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT11.8518518518518516No Hit
GGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATAAATC11.8518518518518516No Hit
CCACATTCCCTTATACTGGAGAACCCCCAAACAGCCATGGAACAGGAACA11.8518518518518516No Hit
CTTCAAAGGAGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.8518518518518516No Hit
CAATTTGGATAGAATTAAAGAACTGAGTGATCTAAAGTCCCAGTCCCGCA11.8518518518518516No Hit
TGTTTCTACTAATACCCCGAGTAGAAACAAGGTCGTTTTTAAACAATTCG11.8518518518518516No Hit
GATCTTCATGATCTCACAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.8518518518518516No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC11.8518518518518516No Hit
GTCTTTATATTGAAGTGTTGCACTTAACCCAAGTGACGTGCTGGGAGCAG11.8518518518518516No Hit
AATATATTCAATATGAACAGAATAAAACAACTGAGACAACAAATGTCCCA11.8518518518518516No Hit
GTAGAAACAAGCGTCTTTTTTATCATAAAATAATCTGAAACGAGAAAGCT11.8518518518518516No Hit
CTGTTCATCCTCAAGAATTCCCATTTTGCATGTGTTGAGAATAGAACTGG11.8518518518518516No Hit
ACCAACACTACGTCCCCTTGCAAAAATAGCACAATAGCCTTCTCTCCATT11.8518518518518516No Hit
AGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTAGATAG11.8518518518518516No Hit
TGCTAGAGTCCCGTTTTAGTTTCATTACCAACACAACGTCCCCTTGCCCA11.8518518518518516No Hit
CTACCTGAGGATAATGAACCAAGTGGGAATGCACAAACAGACTGTGTTCT11.8518518518518516No Hit
CAGGATAATCAACCAAGTGGGTATGCAAAAACATACTGTGTTCTAAACTT11.8518518518518516No Hit
CTTCATATAGACCCCCAAGATAGAAGGTTCCAGGATCCAGGTAGTCCATA11.8518518518518516No Hit
CATGTCGGATGTCCCTCCATCAAAGTTCCCGCTGAATGCTGCCATAACGC11.8518518518518516No Hit
CTCAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.8518518518518516No Hit
GGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATC11.8518518518518516No Hit
GTTCTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTC11.8518518518518516No Hit
ATAAAGAGCTAAACCCTCCCCCTAGTGACACCGGACTTAAAAACAAAAAT11.8518518518518516No Hit
GAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTT11.8518518518518516No Hit
TACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAA11.8518518518518516No Hit
GCCATAACGGTTGCTCTTTCAAAAGGGAGATTCCGCTGCACTCAGAATGT11.8518518518518516No Hit
CCCATGGAAAAGGAACAGAATACACAAAGCACACAGTAAACACAACACAA11.8518518518518516No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAATGAGTTGAACCAAGATGCA11.8518518518518516No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC11.8518518518518516No Hit
CTTATTAGATAGCCTCTCATATACAGTCTTTGTTTTTTCTTCCCTATTGT11.8518518518518516No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA11.8518518518518516No Hit
GTATACGCATTACCATTTCATCGACGAACGGGGTGAATCAATAATTGAAG11.8518518518518516No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGAT50.0145.0145
CCAACAC50.0145.02
ACACTAC50.0145.05
ACCAACA50.0145.01
CAACACT50.0145.03
TACGTCC50.0145.09
CTACGTC50.0145.08
CACTACG50.0145.06
AACACTA50.0145.04
ACTACGT50.0145.07
Produced by FastQC (version 0.11.5)