FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT15.263157894736842No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCAAC15.263157894736842No Hit
CCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGAT15.263157894736842No Hit
GCGTACGACTCTCTGGAGTAAATCAATAACCTGAAATGACTATTGGAGTA15.263157894736842No Hit
ATCTAAGTCCGAGCAACCTCTTTATTCTGTAGATGCTGGGCGGAGAAGAG15.263157894736842No Hit
CCAGTATATTGTGTTGGTGTATTTCAGTTGTCCAAGATTCAGTATCGAGC15.263157894736842No Hit
ACTAATAGCCCTACCTTTGAATGGGTTTGATCCCACAGCTTCTTTAACTC15.263157894736842No Hit
CTCCTCGATTGCTTCATATAGCACCCCAAGAACGAAGTTTCCAGGATACA15.263157894736842No Hit
TCATTGAAGAATCCAACACACGAATAATTGAGAATTAATGCCTTGAAACA15.263157894736842No Hit
CTGCATCTGCACTCCCATTCGCGTCTGGTAGGCGTGCAAATTTTCAAGAA15.263157894736842No Hit
CACACATTCACATATTTGCATTCACTGGAGAGGAGAGGTCCACCAACGCG15.263157894736842No Hit
GTTGTGTTAAATATAATGTTACAACCTGGCCATGACATGAGTAGGTCTAT15.263157894736842No Hit
AATACTTCAATAAGATTCCAATATTTAAATAACAAAATAGAAGCAATAAG15.263157894736842No Hit
AAATAGGAATTAGTCCAATTTGTTTATGAAGGTGGTGCAATCTTTTGTTG15.263157894736842No Hit
CTCCCAACCATTGTGAATTCTTCATAACCTTCATGTACTCTTATTTTAAG15.263157894736842No Hit
ATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCG15.263157894736842No Hit
GTTCTGGGGCGGGCGGGCCCGGGGGGCTGAGGGGAGCGGGTGGGGCAGTG15.263157894736842No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC15.263157894736842No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCAATGCCACAACGACTACAG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)