FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGC11.0752688172043012No Hit
TCCTTGGCCCTTATTCCTCCTTTGGTTCCTTGACCCCAGTGGGCCTCACT11.0752688172043012No Hit
AGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTG11.0752688172043012No Hit
GATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATT11.0752688172043012No Hit
TTCTGGTGCCATATTTTCACCGAGTGCCCCCTTCAAGGGGCTTGTTCGCT11.0752688172043012No Hit
CAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAAT11.0752688172043012No Hit
AAAGGGTACATGATCGAGAGTAAAAGAATGAAGATTCGAACACAAATACC11.0752688172043012No Hit
GATGTATTATGCCCCAATACTCAGCAGCAGGGAAACGAGGATCTTGGAAA11.0752688172043012No Hit
CAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGA11.0752688172043012No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG11.0752688172043012No Hit
GGCATGGCTCGATTCGACCGTGGGTGTCTTTCCACCAGAATCTGGAATAT11.0752688172043012No Hit
TCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCG11.0752688172043012No Hit
GTCACATGTGCCAAGCCCATCCCGCAGTTGCTGGACCAGTGTCTTTGCGA11.0752688172043012No Hit
GTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTTCA11.0752688172043012No Hit
CAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGA11.0752688172043012No Hit
GCGCCGGCGGCTGCGGGGCCGGGGCGGGCGGGGTTTCCGGGGCCGGGGGC11.0752688172043012No Hit
TCCTGGGAGTCGCTGTTCTCCTTTCAGACAGCTGCAGCGGCTAGTAGGTT11.0752688172043012No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT11.0752688172043012No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG11.0752688172043012No Hit
CCGGCTGTGCGTATGCCTTGGGGTCAATTATTGGCTCAGCAATTGACTGC11.0752688172043012No Hit
GGGAAAGGAAAGGAAAAACAGGTTTATGATTTCGAGGGCTGGGGGAAAAA11.0752688172043012No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.0752688172043012No Hit
CCTTTTAAGTCGCCTGTTTTTAAATACCAAGCTCCTTCTCTAGGTGGACG11.0752688172043012No Hit
CACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCA11.0752688172043012No Hit
CCCGCAGCTCTTCGTCAGAGAAGAGGAAGCTGCTGACAGGGAGGTGACTG11.0752688172043012No Hit
AATTGAGACTGGCCACAGGATTGAGGAATATCCCGTCTATTCAATCTAGA11.0752688172043012No Hit
GTCATGTCTTTCTCTTTGACAGAAGAGTCGGCCTCAATCATGCTCTCAAT11.0752688172043012No Hit
TTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTC11.0752688172043012No Hit
CTGCCGTTCGGGGGGGCGCGGTGTGCGGAGGGGGTGGGGTTTTCATGGCG11.0752688172043012No Hit
AACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTC11.0752688172043012No Hit
CTCTGTATCCTACTGAGTGTCTTAGAATATGGACCTGTCACTGACTCCTA11.0752688172043012No Hit
CGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAACCAATGGA11.0752688172043012No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG11.0752688172043012No Hit
CCTGGTGGTGTGGGCACCACCAGAAGAGCCAGACAAGAGACAGGGGAGTT11.0752688172043012No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAT11.0752688172043012No Hit
GTAATCTTCAGGCGGCCAGCGTGGAATTACCTGGGTCACCAGGGCTGACC11.0752688172043012No Hit
GGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAG11.0752688172043012No Hit
ACATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTT11.0752688172043012No Hit
GGCTTGTAGTGGCTCCGGAAAGCTTCATCCTTGGGTTTCTTGGGGTAGAG11.0752688172043012No Hit
CCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATG11.0752688172043012No Hit
GGCATAAATCCCAATTACCTCCTGGCTTGGAAGCAGGTGCTAGCAGAGCT11.0752688172043012No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG11.0752688172043012No Hit
GCTGGCCGCCGGAGGGGTCCAGCGTGCGGCAAATGAGAATGTGGAGCCCA11.0752688172043012No Hit
GTCCCCATACACACCAGGCTAAGGCCTGACGTGGGAGCGGGGGTCATGGG11.0752688172043012No Hit
CAGCTGGCACAGGATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTT11.0752688172043012No Hit
CATCTTCGCCCAGCACGTCCCGTGGGTTCAGAGCCACTCTTCAAGATATC11.0752688172043012No Hit
GTCTATACAACAGCCACTGAAGGAGGTCCTTCTGCTTACTGGCCTTTTAA11.0752688172043012No Hit
GCCGGACTCTGAGCCATATCAGTGAGGCCAGTGTGGACGCTGCCTTGGCT11.0752688172043012No Hit
GGTTTCCACATTCTCATTTGACGGAATCTCGACCCCGCTTGTGGAAAGCT11.0752688172043012No Hit
AAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAA11.0752688172043012No Hit
CCATCCATCTACCATCCCTGTCCACCCCCCTACAATGAAACCGGCAATGG11.0752688172043012No Hit
GTCAGACACGACAAGTTTTTTGTTTTTGGCTCTCATGTCCCATGACCCAT11.0752688172043012No Hit
ATATAGACATCTAAACAATATTGTACTTCCAATCCATGACCTTCCATAGT11.0752688172043012No Hit
GTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTC11.0752688172043012No Hit
AGAAAGCTCTTATCTCTGGTTCTACTTCAAGCAGTAGTTGTAAGGCGTGC11.0752688172043012No Hit
GGATGGGAATGAGATGGGTGTCTGCCCTCTCCAAAGGCATTGTATGGCAT11.0752688172043012No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT11.0752688172043012No Hit
GGGTAAATCAGACATATGTTAACATCAGCAACACCAACTTTGCTGCTGGA11.0752688172043012No Hit
GCTGTGATCTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT11.0752688172043012No Hit
GTATTGACAAGGATGTGGAAAATTTGGGACCCATTGCTGGTGGGAGTGAA11.0752688172043012No Hit
GTTTTTGAGCTGAGCAAGGTCCCGGATTCGATCTCCCAGTGAACGAATGG11.0752688172043012No Hit
GGTCGAAAGGTTGAAACATGGAACCTTCGGCCCGGTCCACTGCAGACATC11.0752688172043012No Hit
GTACCTAACCTATTCAACTGGTGCCCTTGCAAGTTGAATGGGCCTCATCT11.0752688172043012No Hit
CCCTTATACTGCAGATACACCATAAAGCCATGGAACAGGAACAGGATACA11.0752688172043012No Hit
CATCCAGAAACTGATTGCCCCCAGGGAGCCTACCAGTACCAATGAACTGG11.0752688172043012No Hit
GTATGGTGAGGTTAACCCAAGTGCATCTCTATTCTCTTTTCTAAAAAGAG11.0752688172043012No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG11.0752688172043012No Hit
CTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGG11.0752688172043012No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.0752688172043012No Hit
GATTCTACAGGACCTGCAAGTTAGTGGGATTCAACATGAGCAAAAAGAAG11.0752688172043012No Hit
GCCACACATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAG11.0752688172043012No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG11.0752688172043012No Hit
TGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTA11.0752688172043012No Hit
ATCCGGAGTCGGGGCGAAAAGATATCCACAGAGCCAATATGCTGGAGGGA11.0752688172043012No Hit
GTATTTTGTTCTTTTTTATGGCTGAATGATATTGTATTATCTGGATGACC11.0752688172043012No Hit
GTTGTATCCTGGTGCTTGTTGGCAATGCCCAAGCCGGGCCAAGAGGAGGC11.0752688172043012No Hit
GGATTTCTCCTTTCTAGGGAAATTAATCTTCTCCCACTTTCTATCAGGGT11.0752688172043012No Hit
AGCTAATATAAAAATATACTGATGACTTCAAATACTGCCAAAGATGCAAA11.0752688172043012No Hit
GTTTGGGGGAGGGTGGTCATCTTGGAAACAGAGACCAGGTAGGAGGCTTC11.0752688172043012No Hit
GGCTGGACCTGGGCGGGTCCGCTGGGGGGGGGGTGTGGGCGGGGGGTGTT11.0752688172043012No Hit
ATCCTCTGATGCCCAGACCATTGCTACAACTCCATTCTTTCAAGGAAAAA11.0752688172043012No Hit
GGGCTGGTGGGGCCGCTGCAATGGGGAGCGGTTTTATTATTTGGCCAGTG11.0752688172043012No Hit
CAATTAACCACCTTATTTTCTCAAATTTCTGTCCCAATTGCTCTCGCCAC11.0752688172043012No Hit
TCCTGGAGGACAGCCTCGCCGCCACGCAGTTCCAGCAGAGCCAGGCCAGC11.0752688172043012No Hit
TCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCA11.0752688172043012No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT11.0752688172043012No Hit
GTACCTGGATCGGAAGCCAAAGCTGCCTCCGCCGGGGACGCTGGCCCCTC11.0752688172043012No Hit
ACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTA11.0752688172043012No Hit
GGCCGGGGGGGCGGCGGGCGGCGGCGGGGGGGCGGGGGGGCGGGCGCCGG11.0752688172043012No Hit
TAAAAGGGCAGATCAGGCCAGGTGGAGACATCCAATCAGTGGGGCGCCCA11.0752688172043012No Hit
GTAGAAGCAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATA11.0752688172043012No Hit
GCCTGAGACATCTGCATGGCAGCTTTTGGGTTGAGGATTAGCTCCTCGTT11.0752688172043012No Hit
CACATATAGGTGACATATGGGAGACACACAAATTCAGACTAGAAGATCAT11.0752688172043012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGG50.0145.01
GGGAGGG50.0145.07
TTGGGGG50.0145.03
TGGGGGA50.0145.04
GGGGGAG50.0145.05
TTTGGGG50.0145.02
GGAGGGT50.0145.08
GAGGGTG50.0145.09
GGGGAGG50.0145.06
TTTGCTG50.0145.0145