FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA50.1787629603146228No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG40.14301036825169824No Hit
ATTTTATTCTTCCTCTTCCTCCAAGTATGTTTACTTTCCTGCCCAAGACT40.14301036825169824No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT30.10725777618877369No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC30.10725777618877369No Hit
GTGCAGGAATCAGCTTGTGAAGACAACAGAGACTGATTTCAGAAATATAA30.10725777618877369No Hit
GTCTTATATATATTCATTTCAGTCCACTCCAAGCATAGGTTTTTCACCAA30.10725777618877369No Hit
TTCCAGCACCGTTCCGTGTCCTGGTCACAGTTCAATAAGGAGATCCTGCT30.10725777618877369No Hit
CTCTGAGGCAGAGGACTCTGTTGTGGGAGGAGGTGGATTGTGGGCACAAG30.10725777618877369No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT30.10725777618877369No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA30.10725777618877369No Hit
ACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGA30.10725777618877369No Hit
CATATAAACTACCCTTTGGGCTCCCTGCTCTGCCTCTCTAAAACCCCTGC30.10725777618877369No Hit
GCCCAAGACTTACCTTGTTGGCCTAAATAGAAGTGAGAAAAGAAAAAGGA30.10725777618877369No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)