FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.4335260115606936No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG70.3371868978805395No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60.2890173410404624No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT60.2890173410404624No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG50.24084778420038533No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.24084778420038533No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.24084778420038533No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.24084778420038533No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC50.24084778420038533No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.24084778420038533No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.1926782273603083No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.1926782273603083No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.1926782273603083No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.1926782273603083No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG40.1926782273603083No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.1926782273603083No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT30.1445086705202312No Hit
GTTTTCAAAGAATTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGG30.1445086705202312No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.1445086705202312No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA30.1445086705202312No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC30.1445086705202312No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT30.1445086705202312No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT30.1445086705202312No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30.1445086705202312No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.1445086705202312No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC30.1445086705202312No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT30.1445086705202312No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG30.1445086705202312No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA30.1445086705202312No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30.1445086705202312No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT30.1445086705202312No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG30.1445086705202312No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC30.1445086705202312No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA30.1445086705202312No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA30.1445086705202312No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.1445086705202312No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT30.1445086705202312No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA30.1445086705202312No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA30.1445086705202312No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.1445086705202312No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT30.1445086705202312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA100.0065864045144.999982
TACTGAT100.0065864045144.999987
CCTTATA100.0065864045144.999982
CCCTTAT100.0065864045144.999981
Produced by FastQC (version 0.11.5)