FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC25.0No Hit
GGGAAGGAAAACCCTAAATAACTCGTATAGGATATAATACCTAAAAATAG12.5No Hit
TCCCTGTCTTATTTATATAGGACATCTTTTTGCTCATGTTGATTCCCACT12.5No Hit
TATAGGACATTTTCCAATTGTGATCGGATGTAAATTCTGAAAAGGGAGGC12.5No Hit
TCAAAATACAACTCAAGATTTGAGTAACTCAATTTCTCAGCAACTTCTTA12.5No Hit
GTAAAATTGCTCCGTTGATGGTGGCGAACATGCTAGAAAGAGAATTGGTC12.5No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA12.5No Hit
GTATATCCTCCACTCCTGGTCCTTATGGCCAAGTATCTGCTGCTCAGTTC12.5No Hit
ACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCC12.5No Hit
GAATATGGGAGGTTAACTAATAACAGCGCTCCGATTACAAACCATCACCA12.5No Hit
GGTCATTGTTTATAATGTTGTACATAATCAGACTTACTCCAATAATCTAT12.5No Hit
AGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGT12.5No Hit
CCAAGAAGATGGTCACGCAAACAACAATAGGGAAGAAAAAACAAAGACTG12.5No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT12.5No Hit
TCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTAATGCCTTGAAACA12.5No Hit
TCCTGGCGATGATTACATATCTCACCAGAAATCCACCCGAGTGGTTCAGA12.5No Hit
CTACAATTTCATATGACGTCATTTCGCTGATTGCTGATAGGTGTTGGATG12.5No Hit
GGGAATTGACATGCGCTTAGTAGAGAGCAGAGAGTATGCCTTATTTAGGT12.5No Hit
GAGCCAGCCACCAAGTCTCAAACCGGAACGTGTACCTTCACCGGAACACA12.5No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC12.5No Hit
GTAAAATTTAGACCATGCTGCAGGAGTACATGATAGAAAAAGAATATGTC12.5No Hit
GGGGGGGGGTGCAGCGGCAGAAGGGCCGCGGCGCGGCGGGGGGGGCGCCC12.5No Hit
GGAGACAGACTCTTGACGACTGCTATACGGCAAGTGTCGAGTCGCAGGTT12.5No Hit
AAATTTAAATTCAAAGCTACAAACTGGACGAAATATAACCAATCAAATAA12.5No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG12.5No Hit
CGACACTAATTGATGGCCCTCCGAATTCTTTTGGTCGCGGTCTGGCTGTC12.5No Hit
GGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGG12.5No Hit
GGCAGCAGAAAAGGGGAGAAGTGTTATTATTTGGCCAGTGTCAAATGTAC12.5No Hit
CATTACCAACACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCA12.5No Hit
CAGGAATGAGTAGTTTCAACGGAATCATATTCAATGCTTTTGGCTGGACC12.5No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT12.5No Hit
ATAAAAATACAAGAACAATAAATTAAAACACTACCAATATAATCAAAGGA12.5No Hit
GTCAAAGGTAATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATA12.5No Hit
CCATTATACAGGAGATACACAATAACGCCATGGAAAACAAACAGTATACA12.5No Hit
GAAAAGCGCTGAGGGCTGGAATGTCGCAGTCCCGCCCGCGCGGGATACTC12.5No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA12.5No Hit
CTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCT12.5No Hit
CTAGGCGCCGCGGCAATCCTGATGGCGCTGATCAGACTGCAGAGAACACG12.5No Hit
TTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCT12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)