FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA21.6129032258064515No Hit
TCCTTAGACATAATACAACAGAGGATCACGACGTAGACATATGCAACGCA10.8064516129032258No Hit
GTAGGAGGAGGGGCGCGCCTGGGTGGGTGGAGGGGGGCGGAGGGGGGTGC10.8064516129032258No Hit
AACTGGCTCTTTCCACCACGTGGTCAGCTCCAGCCTTTCCGTACAGCCAG10.8064516129032258No Hit
GCAGCAGCGATATGAGGTTCTTGGAAACAGGGCCGCCGGCGCTATGGGGC10.8064516129032258No Hit
GCCTAGAACAAGACTATGTGGGAAGAGTGCTCTGGGGTTGCCTGATGATG10.8064516129032258No Hit
AACTGAGCCAGCGAGGCACCCATATTTCAACTCTTAAGCGGGAAGAAAAT10.8064516129032258No Hit
CCCCTCTCCCCCAAAGACCAGAAAATTGCTCACGGTCCAACTGCCCAGAT10.8064516129032258No Hit
CAAAGAATATAACCTCTCCAGAAAACACAGTTGTCGAAGGGAAGAGAGAT10.8064516129032258No Hit
TATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCAT10.8064516129032258No Hit
GCTTTTTGGATTTCTAATTTAATTCCATCATAGTTCAAGAGCATGTACTG10.8064516129032258No Hit
GGCCTGTGGCAGCCCCAGGGCTGGGAAACGTGCCTGGAATGTCAGCCATG10.8064516129032258No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC10.8064516129032258No Hit
ATATTCTACAAGGAACCTAATAGCCTCAGCAACTGCCATGTGACTGCACT10.8064516129032258No Hit
AATCCTCGGGAGGTCACCGGCCTGGGAATTGTGAGAATTAACAGAACGAA10.8064516129032258No Hit
CCATTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.8064516129032258No Hit
AGCTGGCTCCGCGGCGGGCGGGGGGGCGGCGGCGGGGCTCGGGCGCGGGG10.8064516129032258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGTG10.8064516129032258No Hit
TCCCAGGAGCAGAGAGCCGGATGTGAGGCTCGATCCCAGGACCCTGGGAT10.8064516129032258No Hit
GCAGGGAGCCCTCATTTGCATTTCATTTGCATTTCATTTGCATGCACCTC10.8064516129032258No Hit
CCCCAGCTGTGCACGTGCACGGGATACTCATACTCGTTATGGACTCATGC10.8064516129032258No Hit
CTCCCGTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA10.8064516129032258No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC10.8064516129032258No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTATGGCTTGGGTTGA10.8064516129032258No Hit
CTATAAGGCCAGTATTACCTGATATCAAAACCAGACACACTTCAGCAGAG10.8064516129032258No Hit
ATTTTGAAGAATGGTTCTTATTTACATGTTAAAAGTCCGTACTTCTGAAA10.8064516129032258No Hit
CCACTATAGGATGCAATCAAATGCAGGAAGACATTCTGTGGCTGTAAAGC10.8064516129032258No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA10.8064516129032258No Hit
GGAGAATTTTTATGATGAAACTGAGTATATTAGTACTCTGTGTAGAGAAG10.8064516129032258No Hit
CCGCTTACCAGTATCGTCCACCCTGAGCCGCTGACATTCACAGTCAATGA10.8064516129032258No Hit
ATGCAGAGCTGGTGGTAGTTTAGGACTAATTTCAAGACGGTCTGAAATCC10.8064516129032258No Hit
TCCAAGTGCAGTCAAGCAGAAGAATAAATTCACCCACTAGAGACTTTTGA10.8064516129032258No Hit
TGGCTTTTCCCTCCAGCAAAGCATGAACGTGGGTGTAAGGACCTGTTCAG10.8064516129032258No Hit
CCAGTGGCTGGGTGGCTGGAGAAATGGCAGCCAGGGCCAAAGGGCTGTCA10.8064516129032258No Hit
AGTAGAAACAAGGTCGTTTTTTAACAATTCGCCACTAATTGATGGCCATC10.8064516129032258No Hit
GTAGAGTGTATGACCTACCGAATCACTCAATATTATCAGAGACTCGTACC10.8064516129032258No Hit
GAAGCACCCCGCAAGCAGAAGGAAGTGGATGATGGCAAGGCGATACCCAA10.8064516129032258No Hit
GTCCAGTGGGGCAGTGGGGGGTGGTGACAAGTGCCCAGGTAACCAGAATG10.8064516129032258No Hit
TTTGAATGGATGTCAATCCGACTCTACCTTTCCTATTCCAGCGCAAAATG10.8064516129032258No Hit
GATTAATCCATGGCCAGAGCTGCCATGAAGCCCGGGGGCGGGGGCTTATG10.8064516129032258No Hit
CAATTTCTTTATGCCTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCC10.8064516129032258No Hit
ATGTATATGTGTGTTTAATCCCTGCAGTGTCTTTTAAAGAAAACACCGGT10.8064516129032258No Hit
CTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAAT10.8064516129032258No Hit
GCCTTCCATTCCCTGGTTGAGAGAGACCACCCCAAAGGGCTTTTGGGCAT10.8064516129032258No Hit
GTACACTGGTGAGGATGATTTTAATTTTTATGACTTTTTGTCTGAAACTG10.8064516129032258No Hit
GAGTACCAGCTGGCAATAACAAAATAGAAGAAAGAATAGCTCAATGATTG10.8064516129032258No Hit
GTCCAAACTTCCACCATGCCAATAGCTCTAGCAACAACTACCCAGCAGCA10.8064516129032258No Hit
TGATTGGTTCATCTGGGTGAGGGAAACAGCTGCAATAAAGCTCCATGAAT10.8064516129032258No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC10.8064516129032258No Hit
CCTTCTATCACGACTGGCAGTTAGCCCCGGATCCCAGAGTGAGTGTAGCC10.8064516129032258No Hit
CAATTTTGGCGTTCACTACTTTTTACATTTGGGAAAGCTAGCCCTCAATG10.8064516129032258No Hit
CTTATGATCTCAGAGAACTCTTCTTTCTAGATCCGTCCAGACTCGAAGTC10.8064516129032258No Hit
GTTACTGTAATCTCAAATTTTTCTTCAATTGTCTCTTCGCCTCTTTCGGA10.8064516129032258No Hit
CGCTAGCGGCCGGAGCTGGACGCAGCCAAAGTGGCTGTTAAAGGTTGAGC10.8064516129032258No Hit
ATTGATGGCCATCCGAATTCTTTTGTTCGCTGTCTGGCTGTCAGTAAGTA10.8064516129032258No Hit
ATTATAAGATATTTAGCCGACTGCATGAGGAAGGGTCTGCTGCTGTGCCT10.8064516129032258No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC10.8064516129032258No Hit
GAACACACCAATACTCATAAACGGGAAAGTTGACGACACACAAATAGACT10.8064516129032258No Hit
GTGAACTGCTACCCTATGGGGTTTGCTGATCTTGCTAACTAAGTAGCTTA10.8064516129032258No Hit
ATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCT10.8064516129032258No Hit
CTTTCTGGGTAAGGGTCCTAGTTTATAAGTGAGAAATCTAATCTGTAACA10.8064516129032258No Hit
GGAGCATCCCCAGGAACTCGATGACAGCGACGCCCACACAGATGCCTAGG10.8064516129032258No Hit
GCCCTCTGCTAGCGGCCACTGCCTGGAGCGCCATTTCTGTAGCTGGGATC10.8064516129032258No Hit
AAGAGAGATAACGTTACATGGGGCCAAGGAGGAGTCACTAAGCTATTCAA10.8064516129032258No Hit
TGTTTCTCCTGTCATCCTTACTGCTCACTTCAAAATTCACTTCAGTAAGG10.8064516129032258No Hit
CATTATGTATAGGTTATAATGCGAACAATTCAACAGACACTGTAGACACA10.8064516129032258No Hit
GTGTCTTTTGTCCCTGAAGCAGGGTCTCCCCGTAGCTCAAGGGGGCGCAG10.8064516129032258No Hit
GGCCTTTATTGGTTAGGTAATTAAAAAAAGAAACCATTTAAAACATACTA10.8064516129032258No Hit
ATTTTCTAAAAGTTAGCATTGAGAGGTAAGTCTGATGCTAAAATGTTTTT10.8064516129032258No Hit
CTTCATTGGTTTACGCTTTCTAGTGGTTGCTCTCTGGTTTACAGGTGGAA10.8064516129032258No Hit
TTGTTTTCCTGTGGTGAATCACTCCTCAATCTCAAAGTTGCTACTCAGTG10.8064516129032258No Hit
TGAAAGAATATGGGGAATATCCGAAAATCGAAACTAACAAGTTTGCTGCA10.8064516129032258No Hit
GAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTG10.8064516129032258No Hit
GTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT10.8064516129032258No Hit
CTCTAAGATTCTGGGGAGCCTGGTGGGATGCTCAGGGCAGAGCAAAGAGC10.8064516129032258No Hit
GATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAG10.8064516129032258No Hit
GTCCCAATACTAAAACTTCTCCCCTTGGCGGCTGCCCCCCCAGCACAGCG10.8064516129032258No Hit
CACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTC10.8064516129032258No Hit
CTCTTTGATGGAGGCTAATGCGGGAGGACAGTCTGTGGCTGGCATGAGGC10.8064516129032258No Hit
GGGATGAGTCACTATAAAAATACATACATATGTACATACACAAAAATAAA10.8064516129032258No Hit
CTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATT10.8064516129032258No Hit
CTGACTTACGAGAAGGAGGAGGCACAGGCCCAAGGGGTGAGCTGCAACAA10.8064516129032258No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG10.8064516129032258No Hit
GGGATAATGAGCCAGGGCTAGAGATCAGGGGTGGGCAAAGTGTTCCGGTC10.8064516129032258No Hit
CGTGGGGGGGGGTGGGGCGGGCGGGGGGGGGGGGGGCGGGCGGGGGGGGG10.8064516129032258No Hit
GTTTTGGGGGGCGGGGGGGGGGGGGCGGGGGCGGGGGGGGGCGCGGGGGG10.8064516129032258No Hit
GCCTTCAGCTCAGGTCATGATCCCAGGGTTCTGGGATTGAGCCCCTATTC10.8064516129032258No Hit
CTCATAAAGCATCTACTTCCCAATTTCCCTCTAATCAAAATCACTCTGAA10.8064516129032258No Hit
AGACAATCCATGCTCATGGATTGGAAGAACAATATTGTTAAGATGTCTAT10.8064516129032258No Hit
CAATAGCGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTT10.8064516129032258No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC10.8064516129032258No Hit
AAGCCTCCTCCATTAGCTCCCATTTTAAGCAGCCTTCAGGAATGTGAAGA10.8064516129032258No Hit
CTCCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACA10.8064516129032258No Hit
GACAGCAGTGGGTGGACACTTGGGTGGACATCACGAGAGATGTGATCCTT10.8064516129032258No Hit
GGCCAGCCCTTCATGCACAGAGGTTACGGGGCTGGCCCTGAGCCCCGGCC10.8064516129032258No Hit
AAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGGTCG10.8064516129032258No Hit
CTATATATCCAGAGTTAATTTGGTGTAGTGATTTAGATGCCTTTGCCTTC10.8064516129032258No Hit
GGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTG10.8064516129032258No Hit
GGCCTAAATAGAAGGGATAAAAGAAAAAGGAAGTAAAATACAAATATCTG10.8064516129032258No Hit
CAAGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.8064516129032258No Hit
AGATATTGCAAGGTTGAGGACGGTCTATATTTCGGCACAGTTGACGAACT10.8064516129032258No Hit
CCCTAGACACCATGGCCTCCCCCATGCTAGAAATTCCAACCGGTCTCCTA10.8064516129032258No Hit
GGAAGTAGGCTCCCTGGTAAGCAGAGAGCCCTATTCGAGGCCCGATCCTA10.8064516129032258No Hit
GAATCCAAGTCCCAGCAGTATTTATGCTCAAGTAAGACCCGTACATCGTT10.8064516129032258No Hit
TACATACACCGCACAGACAAGATCCTGAGGAGGTAGAGTCTGAGGACAGT10.8064516129032258No Hit
TTATAATTGAGAAGCTCACTCTAAAGCTATCAGATATCCTCATCTCTTTG10.8064516129032258No Hit
ACCCAAACTGAAAAACAAAAAGACTGGAAAAAATAGTGAACAGAACCTTA10.8064516129032258No Hit
GGTTTAGTTTACTCCCATCCTTTAGAACTCTCCCTTAAATGGGCATAGAA10.8064516129032258No Hit
GTGTGCTATGAGGGAACAGGAACATCTAGATACTTCAGGAAACAAACACC10.8064516129032258No Hit
CAAGCATGAGGAGGAACTATTTTACAGCAGAAGTGTCCCACTGCAGGGCT10.8064516129032258No Hit
GGATAAGAAGATGATCAATCTGTGGAAATTTGGTGCCTTGTAGCACCCCC10.8064516129032258No Hit
ATGCTAAATATGTGTGCAGTTTCTGTGAGCATCAAGCATCCTGACTGATA10.8064516129032258No Hit
GCCTTCCAGAAGTGATTAAAAGAAGAATTTTTATACGAGCTTTTATGCCC10.8064516129032258No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.8064516129032258No Hit
GAGAGAAGCAGGCTTCCCACCGAGTAAGGAGCCCGATGCGGGACCCTGGG10.8064516129032258No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA10.8064516129032258No Hit
CCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACC10.8064516129032258No Hit
CAGTAAGTATGCTAGAGTCCCGGTTTCGTTTCATTACCAACCCTACGTCC10.8064516129032258No Hit
GGGATGGAATACCAATATATGATGCAATCAAATGCATGAAGACATTCTTT10.8064516129032258No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCA10.8064516129032258No Hit
TTCCTGAAGTCTGCTAAAAATGGGAGCAAAAGGATTATGATTATCGGGGA10.8064516129032258No Hit
GCTTAACACTATAAGACAAGAAAAGGCCAGTAGTAGAATATGGGATACCT10.8064516129032258No Hit
AAGATGTGCAGCCTCTCCCGTGATTGTGAGGAGAGGCAAAAGCAGAAGTC10.8064516129032258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT550.00897947213.18181785-89
Produced by FastQC (version 0.11.5)