FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATGTTTCAACCTTTCGACCATTTCCAAATAAGATTTATATACCTTAG13.4482758620689653No Hit
CCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCAATCAAATGCA13.4482758620689653No Hit
TACCAGATCATGATTATACATAGATAAGAGTTGACTAGCACTTACTCAGA13.4482758620689653No Hit
ATCCAGCCATCTGTGCCATAGCCTTTGCCGTAGTGCTAGCCCGCCCCAGT13.4482758620689653No Hit
CCATCCAGCCAATGAACAGGAACAGCATACACCATGGACACAGAAATCAG13.4482758620689653No Hit
ATTATAAAAAAGTCATTATTTCATTACGTAAAAATTAACATCAATAAAAT13.4482758620689653No Hit
GTAGCTTTTTACAATAGATCAATGAACAAAAAAATACAATCGTAAACAAC13.4482758620689653No Hit
GTGGTTTAAAAACTCTGCCTTCGTCTCCGGTGATGATCCCAGGGTCCTGG13.4482758620689653No Hit
AGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACAAAGAAGAAATAA13.4482758620689653No Hit
TAATATATAATGTTGAATACAGATTTTGAAATTAATGTACTGAAAACTTT13.4482758620689653No Hit
AAAGTGGACAATTGTTATTATTTGGACAGTGTCAAATGTAACATGCACCT13.4482758620689653No Hit
GGGCTCGGCCACGGTCAGCACACAGGCGGGCTGTCGACGGGCTTCACATC13.4482758620689653No Hit
CAGCAGTGAGTCACCTTACCCTCTCTATCTCAGACTGCCTCAACTGATCT13.4482758620689653No Hit
TCTTACTATGTTTCTAGCAGCGATAATAAAACTTTGGTCCACATCATCAT13.4482758620689653No Hit
CAAATAAACACCTTAATACCTAAAATTTCTGTAACAAATGCTCTCCACAC13.4482758620689653No Hit
GTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAAGAG13.4482758620689653No Hit
TTGGATGTGTGAAATTAAAACGGAATAATAAAATAAACTATCAAGAGTTG13.4482758620689653No Hit
AGCAGGTACTGATCAAAAATGTAAGACTATCTTCGACAATGCTAAAATCC13.4482758620689653No Hit
AATCCAATGATCGGCGAGCTTGCGGAAATGGCAAGGCAAGAATATGGGGA13.4482758620689653No Hit
GATTTTGCCAGTAAGTTCCTGCCCCATTTCCCAATAGAGCCTTCATCAAC13.4482758620689653No Hit
GTTAAACCTACAGTTTCTCTTGTTTTTAAACAAATACAGCAACTATAGAA13.4482758620689653No Hit
GAGGAGAAGCAGACTCTTTGCTGAGCAGGGAGCCTGATGCAGGGCTCGAT13.4482758620689653No Hit
AACTGAGTACTAGGGGCTCTCGAATCAGAAAACTGCCCTAAATAACTATC13.4482758620689653No Hit
AAACCCTGCCTGTTCCGCCGGCTACTGGGAGAATCCTTGTTTTACGGACC13.4482758620689653No Hit
GCCTGTCAGAGCTAAGAGTCACTCTTTTCCTTCACAAACTAAAGGGTGTC13.4482758620689653No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGCTGC13.4482758620689653No Hit
AGTATCAAGAATACACAATGGTTTTTATAATAGAAAAATCTATTATCAGA13.4482758620689653No Hit
ACCAAGGAACTGAGAAGTAGACAATAACTTATTCCTCATCAATGATGTGG13.4482758620689653No Hit
ACATCAGGAACGCAAGAGAATAACCCCGCAATCAGAAACAACGCCATGAT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)