FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.3205128205128205No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.2403846153846154No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT30.2403846153846154No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG30.2403846153846154No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA30.2403846153846154No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.16025641025641024No Hit
CAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATG20.16025641025641024No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.16025641025641024No Hit
CATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTG20.16025641025641024No Hit
GTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTG20.16025641025641024No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT20.16025641025641024No Hit
CTTCAATTGGCTTGTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTT20.16025641025641024No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.16025641025641024No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG20.16025641025641024No Hit
ATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTCATTA20.16025641025641024No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC20.16025641025641024No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA20.16025641025641024No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.16025641025641024No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC20.16025641025641024No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC20.16025641025641024No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20.16025641025641024No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT20.16025641025641024No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG20.16025641025641024No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC20.16025641025641024No Hit
CAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAAT20.16025641025641024No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA20.16025641025641024No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATC20.16025641025641024No Hit
CCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCTTA20.16025641025641024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)