FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCC11.3513513513513513No Hit
TTCTTATAACTTCTTTTCGCATGTCGGATGTCCGTCCTTCATTGTTCCCG11.3513513513513513No Hit
CACATTACAGTAAAGCAAGAACCATTTACACAAGCACATACAGACTCTTG11.3513513513513513No Hit
GGCAATGAGATACCCAAATACAGCAGACAAGAGAATAATGGACATGATAC11.3513513513513513No Hit
CTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCG11.3513513513513513No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGTTGATGATGGGCAT11.3513513513513513No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG11.3513513513513513No Hit
AAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTT11.3513513513513513No Hit
CCTTATGGCCCAGTAACTGCTTATCAGTTCCAGCGTATTGGAGTCCATGG11.3513513513513513No Hit
GGTAAGATACCAAATAATATGCATATTATTTTGATCTCTGGAGGCATATC11.3513513513513513No Hit
GGATGAATTGATAAAGGCCGTATTTCTGTACCCTCACTTCAGTATGTATT11.3513513513513513No Hit
GTAAAAATAGACAGAAACATTAGAGTATTTTGATAATCAATCAACTTAAT11.3513513513513513No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA11.3513513513513513No Hit
CCAAACTGTCAATTGTTGTGAGAAAGAAGATGACTAATTAACAAGACACA11.3513513513513513No Hit
GTATATTTTCAGACACTCGAAACGTGTTACCATTCCATTCAAGTCCTACT11.3513513513513513No Hit
GCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCTTCGT11.3513513513513513No Hit
GAAGACGGAAAACAAGCCTGTATGGGTTCATTTTAAAAGGAAGGTCTCAT11.3513513513513513No Hit
GCGTTCTTAAATGGTGGAACAGATCTTCATTATCTCAGAGAAATCTTCAT11.3513513513513513No Hit
ATTTAACGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCAAC11.3513513513513513No Hit
ATATTCAATATCGAGAGAATAAAAGAACTCAGAGATCTAATGTCGCAGTC11.3513513513513513No Hit
GTCCTCAAACTGATACTAATAGCCCTACCTTTTAATGGGTTTGATCCCAC11.3513513513513513No Hit
GAAGGGTACTCCCTGGTCGGGATAGCCCCATTCAAATTACTCCAAAACAG11.3513513513513513No Hit
GGAAAATAGTTACTCCTCATCATTGCGATATGTTCAATCGGGGCAACATA11.3513513513513513No Hit
GTATGGATAGCAAATAGTAGCATTGCAACAACTACTTCAGTGCATGTGTG11.3513513513513513No Hit
CATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGCAGCTGCTT11.3513513513513513No Hit
TATTTTAACTTGATTTCTGAAGTGGACAGGGCCGAAGGTACCATGTTTCA11.3513513513513513No Hit
TATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGG11.3513513513513513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
GATTGGAGCCCATCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCC11.3513513513513513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
TGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCA11.3513513513513513No Hit
GTTGTTCCACAAAAAAGGGTAGCTAAACTAACTCAAGGTAGCCAGAATTA11.3513513513513513No Hit
ATCCTGGGCGGTTAGGACCTGGAGTGGAGGGTGGACCAATCCTGCAATGG11.3513513513513513No Hit
CTGCATCCCATGACTCAGTGATGCTGTGCCGGCTCTTAGAATAGGGCTTA11.3513513513513513No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGCCCCTGGCATCACTCCGGGCCCT11.3513513513513513No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAATATTACACTGAAGTTATATTTC11.3513513513513513No Hit
GGATTTTACTTCACATAATGAAGAAAGGGGAGGCCTGTTCTGGCTTATAT11.3513513513513513No Hit
GTTATTCAGATTAGATTTTTCTTATACTTTGTTGCAACTTTAGCTATGAG11.3513513513513513No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11.3513513513513513No Hit
CACTGAAAGCCCTTAGTAGTATCAAGATCTATAATCGGTTAAACATTACA11.3513513513513513No Hit
GTGATGGCTAGAACTACGTCAAAGGAAATTTAACAGATGGCTGGATCCTG11.3513513513513513No Hit
CGAATATTTGAGAAATCATGACTGGAAACAATGCAAGTTTTACAACAAAC11.3513513513513513No Hit
GTCAGTAAGTATGCTAGAGTCCCGGTTTGGTTTCATTACCAACACTACGT11.3513513513513513No Hit
AGGTCCAAGGTCATTGTTTATCATGGTGTTCTTTATCACTGTTACTCCAA11.3513513513513513No Hit
CACCAATACTCACAAAAGGGAAAGTGGACGACAAGCACCCAGACTGGTGC11.3513513513513513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGCGGG11.3513513513513513No Hit
GGGGAGGCAGGGGGCCGGAGGGGTCCGAAATGGGAGGGTGGGGGTCAGAG11.3513513513513513No Hit
ACTGAACCCCATGCACATACTCATGAGGCATTTCCAAAAAGATGCAAAAG11.3513513513513513No Hit
CTACGGCAAAGGCTATGGAACAGATGGATGGATCGAGTGAACAGGCAGCG11.3513513513513513No Hit
ATTGACAAAGTTCAGATCGCCCCTAACTGCCTTGATCATGCAATCCTCCT11.3513513513513513No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCG11.3513513513513513No Hit
GATCGGGAGTCAGCATTTAGAAGGAAGAACCAAATCTTGTCAAAAAGATT11.3513513513513513No Hit
AATTAAGCCAGTGTACATAAAAACTGACAAGATCAATGCAACCAAATCAG11.3513513513513513No Hit
GGTCCAGATTGCTTCAAATGAGAATGTGGAAGCCATGGACTGCAATACCC11.3513513513513513No Hit
GGTTCAGAAACATCCTGAGCATGGCACCCATAATGTTCTCAAACAAAATG11.3513513513513513No Hit
CTTCATACCCTTCAAGTACTCGTATTTTAAGTTTTTGTAGGTTGCCCGTT11.3513513513513513No Hit
TCTTGTCTTATAGTGAAAAGCCTAGTTTTGATTCTTGCCCTGCTCTCTTC11.3513513513513513No Hit
AGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTAT11.3513513513513513No Hit
GCATTAAACAGAATTATGCGGCCAGCTACACCGTTCGGCTTCAGCATAGG11.3513513513513513No Hit
AAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGT11.3513513513513513No Hit
GTTCTGGAAAAGCACTTTTCCATCATTTTGGAAATGCCTCAAGAGTTGGT11.3513513513513513No Hit
CATCATTAATGCCGAATAAGATATACACAACTAATCACATCCTTGCGTTA11.3513513513513513No Hit
GGGGGGGCGCGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
TATGAAACTTGATACTCTTAAATCTTAAAATTCAGAAGAGAGGCATGCCA11.3513513513513513No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC11.3513513513513513No Hit
CTGGGGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTA11.3513513513513513No Hit
CCAGAGAAGTCGTTAGATCGAATGGCCTTACAGCTAATTAGTCAGTAAGG11.3513513513513513No Hit
ATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCAC11.3513513513513513No Hit
ACCCTTGGGGGCGTGATCTGAACGGCGACAATTGTTGGGTGACAACACGG11.3513513513513513No Hit
GAGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAAT11.3513513513513513No Hit
GGCTTGAACTTCTTGCTGTATCTAGATGACCCCACAAAAACATATGCATC11.3513513513513513No Hit
ATCCAGTTTGATTAGAGGGAAGGAAGTGATTCCGGGGGGGAGGCTTTCCG11.3513513513513513No Hit
AGAATGGAGTTCTCACTCACTGCCCCGAGACTGGAGCCACACAAATGGGA11.3513513513513513No Hit
GACCCAAGTGGGTATGCACAAACATACTGTGTTCTAGAGGCTATGGCTTT11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTCA50.0145.02
TCAGATT50.0145.06
TATTCAG50.0145.03
TAACAAG50.0145.0145
CAGATTA50.0145.07
GATTAGA50.0145.09
GTTATTC50.0145.01
TTCAGAT50.0145.05
AGATTAG50.0145.08
ATTCAGA50.0145.04
Produced by FastQC (version 0.11.5)