FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGCTAAAGTGCCAGGAGAAATGACTAGGAAGGTGGGAAACCCTC40.2976190476190476No Hit
CCCTAAGGTGTCTCCAAAGCGTGCCTGACGATTTACCAAGCACTTTCCGT30.2232142857142857No Hit
CTCCTACCCCGCCCTGCACTCCTCACGGCGGCGACCGCTCTGGAAAACCC30.2232142857142857No Hit
GCCTAGGACAGAGTATGCCAACACTGAAAAAACAGAAAAACAGAAACCTA30.2232142857142857No Hit
GAATAGAGAAGAATGCCAGGAGGAATGAGAAAGGATCAGCAAAACATTTA30.2232142857142857No Hit
ATATCTGCTTGTTTTAGCCAGTGACAAGCCTTATCAAAATCTTCCTTAAA30.2232142857142857No Hit
ATTAGAACTTCATTCCTTTTTAAGGTTAAGTAATAGTCCATTGTGTGTCT20.1488095238095238No Hit
GTCCCAGCAAGGACGTCATCCACTGGGCCCTCTGGCATCCTCTGAAAGGT20.1488095238095238No Hit
GTCCTCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCCATGAGTCCCCAC20.1488095238095238No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA20.1488095238095238No Hit
GGTTAGGAAAGCCCAAAGATTGAGGATAAAAAGCCTAGGACAGAGTATGC20.1488095238095238No Hit
ATGTACCACTGTATCCTCACTCTCTCCTCACCCATATTTCCTAACTACCT20.1488095238095238No Hit
GTATTAGGAATTGAGTATGATCCATTAGGAGCGACTCACACCTCAGCATA20.1488095238095238No Hit
TAACAAGACAGTAATCAAAGATGCTATTTCCACCCCCTCCTTAAGATAGC20.1488095238095238No Hit
AAGCAGGATGGCACCGTTAGGAAGCAGAGCCGAGGAGCACAAAGCGGGCA20.1488095238095238No Hit
CTCCAATTTAGAGAAAACTTCTAAGCACAGAATTAATTTATCCTGAACCA20.1488095238095238No Hit
CAATAACGTAGGTCTTCAGTGAAATTTTAGCGTGTGCTTTTGTCATACTG20.1488095238095238No Hit
GTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGC20.1488095238095238No Hit
GGCTCTGTGACAAGTTCAGCAGAGAAACATGACTTGGTTACAGCTAACAC20.1488095238095238No Hit
TCCTAACCCTGCTGGAGACAGACGGGGGCCCAGCCGGTCTGGACGACGGG20.1488095238095238No Hit
AACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACT20.1488095238095238No Hit
CTGCCCACGCTAGCTGGCCCCCAGGCGAAGAGGCTGTCGGCCTCCAAGCG20.1488095238095238No Hit
GGAGAAACATGGGCAAACAGATGCCACAGCCGACCTTCACGCTGCGGAAA20.1488095238095238No Hit
GTTCAAACCACAGTCATGGATTTCAAACTTCCTTTTGACCTCACATTCCC20.1488095238095238No Hit
GAGGAGATGAGACCATGAGGTTCTTAGCAAGGTGGACGGGGGTGGACAAA20.1488095238095238No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC20.1488095238095238No Hit
GTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATTTGATTCCCT20.1488095238095238No Hit
GCCCGTGCAGAGTGCAGAGTGCTGGCTGAGGGAGGAGACTAGTCCAACCA20.1488095238095238No Hit
GGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCT20.1488095238095238No Hit
GAGGTCCTCTTGACCTTGCTCAGGGCCCAGGTGAGGGACCCGTGAGCCTG20.1488095238095238No Hit
TTCCCACCCTGGAGGAGCAGCAGCCGAGTCGGACCAGGAGGTGCCTCTGT20.1488095238095238No Hit
GGTGGGGAGGTGGCTGTCAGCCAGGCGGGAATGACCAAGCTGCAGCGGGC20.1488095238095238No Hit
ATCTTGAGTAAACCAAAGTTAAGTTATTAAGTTCCGAAGCTCCTCCTTTA20.1488095238095238No Hit
GAATTAAGATACAGCTGGGTTCTCTTTGTGGAGCCACCTTGCCTCTCTCT20.1488095238095238No Hit
AATACAAACAGCTCGAGGAAGGCAGGGGAGCATTTTGGAAAATGCCAGCC20.1488095238095238No Hit
GGCCTGTCACGAAGAGGTAGGCAAAAGGCTCCTCTCCTAGTGACCCAGGG20.1488095238095238No Hit
CGTCAGGGATGGGTTTGGGCTCCCTTGAAGAGCTGTCCTCAAGCAGAATG20.1488095238095238No Hit
TCTAGCTTCCCCTCTTGCCTCCCTGGAACCTGGTCTTAAATCAGAACCCG20.1488095238095238No Hit
CTCTTTAAACTATTGATCAGAAGTGATTATTAAGGGTAGAAAATGGAAAT20.1488095238095238No Hit
GGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAAA20.1488095238095238No Hit
TCCCAGTATTTCACGCCCCTTCTTATACATGCCCTTAAGGACCATCTGAA20.1488095238095238No Hit
CATCTATGGAGCCCAATCCATTGGAAGTTCCCTGATCTGCCACGCCAGCC20.1488095238095238No Hit
ACCCTGTTTTCTTTCTCTGGACAACAGTGCACTCCCACCCCTGTCTGCAG20.1488095238095238No Hit
ATCTAAACATGGTAGGTTCTCATTAACTGCAAATGAATATTGAATGCACT20.1488095238095238No Hit
GTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCA20.1488095238095238No Hit
GAATACAGAAATGAGGTCAAGAGAGGACAGAATGGGTTCCTGATGGCTTC20.1488095238095238No Hit
TCTGGATACGGGAGGGAGATGTGGATAGAATGCTTAACAGGAGCTGGAAA20.1488095238095238No Hit
TTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCC20.1488095238095238No Hit
TTTTAATGCTGGTGGGGGATCCCCGAAACTTTTAAATAGGGCAGTGACAT20.1488095238095238No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)