FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT21.4925373134328357No Hit
AGCGCGGACTGCAGCGTATACGCTTTGTCAAAAATTCCCTAAATGGGAAT10.7462686567164178No Hit
TGAAACAACTCTTGATGCATTTCCAAAAATATGCAAAATTTAATTTCCAT10.7462686567164178No Hit
ACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTA10.7462686567164178No Hit
GTGATATATGAAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATT10.7462686567164178No Hit
GTAACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCAAGGATTGTA10.7462686567164178No Hit
CAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGGCA10.7462686567164178No Hit
GACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGT10.7462686567164178No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG10.7462686567164178No Hit
TAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGAC10.7462686567164178No Hit
CAAAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACCACGC10.7462686567164178No Hit
CAATAAGGGATTGGAATGACCTGCTAGAAGCGGGAGAGGACATGAAGAAT10.7462686567164178No Hit
GCATGTTGAACATGCCCAACATCATACCAGTACTCAGTGATGCAGTGCCA10.7462686567164178No Hit
GTGAAAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.7462686567164178No Hit
CGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAACTGTGAT10.7462686567164178No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGATGCAAGTTAAAAAGCAGG10.7462686567164178No Hit
CCTCCATCTGATACAAATAGCCCTACCTTTGATTGGGTTTGATCCCACAG10.7462686567164178No Hit
GACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTGT10.7462686567164178No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA10.7462686567164178No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10.7462686567164178No Hit
CTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATATT10.7462686567164178No Hit
ATGTAGATGGATTCGAGAAGCAAGGATGCATTGAGGGCAATCTTTCAAAA10.7462686567164178No Hit
CTTCCATTGTTTCAAGGCATGAATTCTCAAATATTCCTGGGTGGGATTCT10.7462686567164178No Hit
ACCAGCGACTGAACACCATGCACAAACACTTGACCCAGTTCAAAAAAGAT10.7462686567164178No Hit
CTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGAT10.7462686567164178No Hit
GAGACAGAATCATGGACTGGACAGTGGTGAACAGTATATGTAACACAACA10.7462686567164178No Hit
CAGTAAGTATGCTAGAGTCCCGGTTTAGTATCAATTACAACACAAACAAA10.7462686567164178No Hit
GTATAAACTATCCTCATTATTTTAACTTGATATCTCAACTGGACAGGGCC10.7462686567164178No Hit
GTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCA10.7462686567164178No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT10.7462686567164178No Hit
ACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGG10.7462686567164178No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT10.7462686567164178No Hit
CCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCAG10.7462686567164178No Hit
GGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATA10.7462686567164178No Hit
CTGCTGGTCAATCTGATCATACGCAATAATTAAGATTTTTCTTGTTGAGA10.7462686567164178No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG10.7462686567164178No Hit
GTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTTCGAGAGTA10.7462686567164178No Hit
GGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGACAATAT10.7462686567164178No Hit
CTCAAATCTTGAGTTGTATGGAGAGGGAACTTCACCAATAGGACAGCTCA10.7462686567164178No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.7462686567164178No Hit
GCCTAGTTTTGATTCTTGCCCTGCTCTCTTCGTCAAGGGTGTAGTCCGCT10.7462686567164178No Hit
ATCTTAGGGGGAGTGGTGTCTTCCTCAAGAATGGTTCAATTTTGTCGTTC10.7462686567164178No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT10.7462686567164178No Hit
GAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTACAT10.7462686567164178No Hit
CTTCAGAAACATCCTGAGCAAGGCACCTATAATGTTCTCAAACAAAAAGG10.7462686567164178No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT10.7462686567164178No Hit
TGATTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAAT10.7462686567164178No Hit
GTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATAT10.7462686567164178No Hit
GAAGAAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAG10.7462686567164178No Hit
GAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACA10.7462686567164178No Hit
GTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTG10.7462686567164178No Hit
ACCATAAATCACCCCGAGTCTTTCAGAATCATCCACAGCAGCGCACCGAT10.7462686567164178No Hit
ATTAAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10.7462686567164178No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT10.7462686567164178No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC10.7462686567164178No Hit
ATACTGACATTAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGA10.7462686567164178No Hit
AAACAACTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCA10.7462686567164178No Hit
GCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTGTCAAATGTCCCAAG10.7462686567164178No Hit
GGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATT10.7462686567164178No Hit
GTGGACGACAACCACAGAGACTGGTGCACCCCCGCGCACCCCGATTGATG10.7462686567164178No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC10.7462686567164178No Hit
CGCCCTAGACACCATGGCCTCCACAATGCTAGAAATTCCAACCTGTCTCC10.7462686567164178No Hit
ATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATT10.7462686567164178No Hit
CAGTTCCAGCAAATGCGGGAAGTGCTTGGGACATTTGACACTGTCCAAAT10.7462686567164178No Hit
CTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTT10.7462686567164178No Hit
CAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCC10.7462686567164178No Hit
CTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTAT10.7462686567164178No Hit
GCCATAAGCACCACATTACCTTATACTGGAGATCCTCCATACAGCCATAT10.7462686567164178No Hit
GCATTGTTTACAGATTCAATCTCTTTATGACTGACAAAGGTATTCAGGGG10.7462686567164178No Hit
CGTCATGATCTAAGAGAACTCTTCTTTCTTGCTCCGTCCAGCCTCTAAGT10.7462686567164178No Hit
CAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAG10.7462686567164178No Hit
ACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAA10.7462686567164178No Hit
CCCAGCTCACAAAAGTCAAATGTAGAGGATTGCAATCAACTCTCATGCAT10.7462686567164178No Hit
AATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTC10.7462686567164178No Hit
TTTCATCTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT10.7462686567164178No Hit
CAGTCACTCCTTACGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGA10.7462686567164178No Hit
AAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGC10.7462686567164178No Hit
ACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACT10.7462686567164178No Hit
GCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGG10.7462686567164178No Hit
ACCAGGTACAAATCCAAAAAGTAAGACATTTTCCCAAAATGATTCAATCC10.7462686567164178No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG10.7462686567164178No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGTAGAGAATAAAAGCACTGAG10.7462686567164178No Hit
CCTTAGTGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGGTTAAA10.7462686567164178No Hit
CCCAAAGCTCAGCAAATCCAACATTAATGATAAAGTGAAAGAATTCATCG10.7462686567164178No Hit
GATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGA10.7462686567164178No Hit
ACACTAAGTGGAATGAAAATCAAAATCATAGAATGTTCCTTGCGATGATT10.7462686567164178No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC10.7462686567164178No Hit
ATGCTAATGGGTCTGCTTACACTGCTGCTCTTCTTACTATGTTTCTAGCA10.7462686567164178No Hit
ATATCGAGACTCCCAATACAGTACTTAGCATGTTGAACATGCACATCATA10.7462686567164178No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCCTTCGATCTAAAGACT10.7462686567164178No Hit
ATATTGCTCTCATGGTGAATGCACCAAACCATGAGGGAATACAAGCAGGA10.7462686567164178No Hit
GTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGGAACCATC10.7462686567164178No Hit
CATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCATGTTGTTCTTTA10.7462686567164178No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT10.7462686567164178No Hit
AGTAAATGTCACTAGAATGGTTCATTCTCATTCATATGCAAAAGATAATA10.7462686567164178No Hit
CTCATTTGAGAAATGATACGGATGTTGTGATCTTTGTAAGTATGGAGTTC10.7462686567164178No Hit
ATCTAGCCCCCCAAGATCGAAGGTCCCATGTTCCAGGTGGTCCCTAAGTG10.7462686567164178No Hit
ACTACCTGAGGATAATGAACAAAGTGTTTATGCAAAAACAGACTGTGTTC10.7462686567164178No Hit
GAGTATTGGAGTAACAGTGATAAAGAACAACAGGATAAACAATAACCTTG10.7462686567164178No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC10.7462686567164178No Hit
ATGATACTGATGTGGTTAACTTTGTAAGTATGGAGTTCTAACTCACTGAC10.7462686567164178No Hit
ATTGGAGCCCATCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCA10.7462686567164178No Hit
GTATATCTACCATTCTTTGTAGAACACATAACAAATATTCAAGACATCAA10.7462686567164178No Hit
CCAGATCAGTGTGCAGCCTACATTCTCAGTGCAGCGGAATCTCCCTTTTG10.7462686567164178No Hit
TTGAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTGTCC10.7462686567164178No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.7462686567164178No Hit
GCTTTGGAGTGTATGTAGTAAATTAATAAGATTACATGAGTATTGGATTA10.7462686567164178No Hit
CTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACACCAATTGA10.7462686567164178No Hit
GCACAGATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTCTGTC10.7462686567164178No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA10.7462686567164178No Hit
ATAGCAACATGGTCATCGAACATTTGCATATCTCAGTCCTCAAGAGCATG10.7462686567164178No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC10.7462686567164178No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCAATAGCGATAAAAAAAGC10.7462686567164178No Hit
ACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAA10.7462686567164178No Hit
GAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTAT10.7462686567164178No Hit
GTCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGAAGAGAGA10.7462686567164178No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT10.7462686567164178No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC10.7462686567164178No Hit
GTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATA10.7462686567164178No Hit
GTAGGGGGCAATGAAAAGAAGGAAAAACTGGCAAATGTTGTGAGAAAGAT10.7462686567164178No Hit
GTCTATGGTATTACTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA10.7462686567164178No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10.7462686567164178No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT10.7462686567164178No Hit
AAACTTCAATAATCCCATCCTGTGCCTCCTTGGCACTGAGATCTGCATGG10.7462686567164178No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7462686567164178No Hit
ACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACT10.7462686567164178No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC10.7462686567164178No Hit
ACAAAGCCCGTTGTCCCCCCGAACCATAGGCCGTACTGACCCAGCTCTTC10.7462686567164178No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT10.7462686567164178No Hit
ACACAGGACCCACCCTAGCAAACACAGGAAAAGGACGGACAGTCATCACA10.7462686567164178No Hit
ATTTGAGAAGTCATGCCTTGAAACAATGGAAGTGGGTCAACCCGCCAGGG10.7462686567164178No Hit
ATGTTCACTCGATCCAGCTATCTGTTCCAAAGCCTTTCCAGTAGTGCTAG10.7462686567164178No Hit
ATATGGACCCCTCATGTGGAAAGCTTTCCTCGTGGAATCACTGTCTTTCC10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers