FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC100.3852080123266564No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.30816640986132515No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT70.2696456086286595No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT60.23112480739599386No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA60.23112480739599386No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.23112480739599386No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.23112480739599386No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT50.1926040061633282No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT50.1926040061633282No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG50.1926040061633282No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.1926040061633282No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.1926040061633282No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGT50.1926040061633282No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG50.1926040061633282No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC50.1926040061633282No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG50.1926040061633282No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG40.15408320493066258No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA40.15408320493066258No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.15408320493066258No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.15408320493066258No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA40.15408320493066258No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT40.15408320493066258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.15408320493066258No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT40.15408320493066258No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC40.15408320493066258No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC40.15408320493066258No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA40.15408320493066258No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA40.15408320493066258No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC40.15408320493066258No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC40.15408320493066258No Hit
CCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGCA40.15408320493066258No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.15408320493066258No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT40.15408320493066258No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.15408320493066258No Hit
CACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCA40.15408320493066258No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.15408320493066258No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.15408320493066258No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT40.15408320493066258No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.15408320493066258No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.15408320493066258No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA30.11556240369799693No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG30.11556240369799693No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC30.11556240369799693No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATG30.11556240369799693No Hit
CCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCAT30.11556240369799693No Hit
GATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAA30.11556240369799693No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA30.11556240369799693No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG30.11556240369799693No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.11556240369799693No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC30.11556240369799693No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA30.11556240369799693No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC30.11556240369799693No Hit
ATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTCATTA30.11556240369799693No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA30.11556240369799693No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA30.11556240369799693No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA30.11556240369799693No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.11556240369799693No Hit
ATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGC30.11556240369799693No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA30.11556240369799693No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC30.11556240369799693No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.11556240369799693No Hit
AGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGG30.11556240369799693No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT30.11556240369799693No Hit
GTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCAC30.11556240369799693No Hit
CCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCG30.11556240369799693No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT30.11556240369799693No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC30.11556240369799693No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT30.11556240369799693No Hit
CTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATATT30.11556240369799693No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.11556240369799693No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC30.11556240369799693No Hit
CTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGG30.11556240369799693No Hit
GAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAG30.11556240369799693No Hit
CTCCAGTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTG30.11556240369799693No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30.11556240369799693No Hit
ATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGA30.11556240369799693No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA30.11556240369799693No Hit
GTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACT30.11556240369799693No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA30.11556240369799693No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA30.11556240369799693No Hit
ACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAA30.11556240369799693No Hit
GGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACA30.11556240369799693No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT30.11556240369799693No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30.11556240369799693No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT30.11556240369799693No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.11556240369799693No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30.11556240369799693No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA30.11556240369799693No Hit
TGTTGATTCCCACTAACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCT30.11556240369799693No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG30.11556240369799693No Hit
AAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGC30.11556240369799693No Hit
GTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGGGAAGGGTACT30.11556240369799693No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA30.11556240369799693No Hit
CTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACACCAATTGA30.11556240369799693No Hit
GTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAA30.11556240369799693No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG30.11556240369799693No Hit
ATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCT30.11556240369799693No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCAT30.11556240369799693No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA30.11556240369799693No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC30.11556240369799693No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC30.11556240369799693No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT30.11556240369799693No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG30.11556240369799693No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.11556240369799693No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.11556240369799693No Hit
AGTCAATTCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAG30.11556240369799693No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC30.11556240369799693No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA30.11556240369799693No Hit
GTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTT30.11556240369799693No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.11556240369799693No Hit
CTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCC30.11556240369799693No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA30.11556240369799693No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.11556240369799693No Hit
CTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTT30.11556240369799693No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.11556240369799693No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA30.11556240369799693No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA30.11556240369799693No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA30.11556240369799693No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.11556240369799693No Hit
CTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGA30.11556240369799693No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATA30.11556240369799693No Hit
GTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCT30.11556240369799693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAGT100.006684254144.999984
GTAACAG100.006684254144.999983