FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGTAATTACCACCAGATACATGAACAGAACTTCCCCATCGTGAACA14.545454545454546No Hit
TCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCT14.545454545454546No Hit
GTAATAAGTCCCATTTTTGACAATAACCATGCACATTTTATCTAATTAGT14.545454545454546No Hit
CTAAAGGTCGCCAGACTTATGATTTGACATTAAACAGCAATCAAACGGCA14.545454545454546No Hit
GGCCCATAGATTGAATAGACGGGATCTTACTCAATCCTGTGGCCAGTCTC14.545454545454546No Hit
CTGAATAACCACTTCACAGCACTAAACATATTGCTGAGTAAACTTTAATC14.545454545454546No Hit
GATTATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATGAAACGGTTCT14.545454545454546No Hit
GCTACTACCACCAAACCACTAATCAGGCATGAAAACAGAATGGTGCTGGC14.545454545454546No Hit
AAGGAATATCTTTGCAGCAAGACCCAGGGGCGCTGTATGACTTTAATTTA14.545454545454546No Hit
CTCATCACAGTTTATCCAATCGAATCTATGGATATTTTCAGAGAATCGAA14.545454545454546No Hit
TTGGTAGACAATGATTCAATCAAATGATAATATATCTTGCCTGAAATGAA14.545454545454546No Hit
TTACAATGAAGTTCTCTTTCAGTAATATTTTATTTTCCATGCTCGACGGG14.545454545454546No Hit
ACATAGTCCGACAATGCTTAAATCCNATGATCGTCTATCTTGCGGAAAAG14.545454545454546No Hit
CATCCCACAAGAATATTGACTTGGGGTAGATCTTTTGTCCAACATAAAGA14.545454545454546No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAAATAAGACCCTTCATTAC14.545454545454546No Hit
CGCCCACCGTGCCCAGAGAGCGAGGACTGCAGCCTAGACGCTTTGTCCAA14.545454545454546No Hit
GTTAAAAACAAAAAAAATATCACCAAAGACCTTCCACATGCAACTGCCAA14.545454545454546No Hit
GATCAAAAGCATGGTGACAAAAAAATAATGTAATCCAAAAACATGTCAAG14.545454545454546No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA14.545454545454546No Hit
GATCATATGAGTTAAACACGCTGACGGATCAAACCCAATCAAAGGTATGG14.545454545454546No Hit
GTCTTACAAATATCATGATTTGTCATTCAAACCGGAAAGATGCCAAATAA14.545454545454546No Hit
CTGTTATACAGGAGATGCTGTATACAGTCTCTTAACAAGAATCTGATACA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)