FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R1_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA18.333333333333332No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG18.333333333333332No Hit
TCAATACTCAATACCACTCTCTCCGTGCTGGAGTATTCATCTTCTCGCAT18.333333333333332No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC18.333333333333332No Hit
CTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCT18.333333333333332No Hit
ACAAAGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG18.333333333333332No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA18.333333333333332No Hit
CAAGCCGTAGACATATGCAACGCAGCAATAGGGTTGAGGATTAGCTCATC18.333333333333332No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG18.333333333333332No Hit
TCTCAAGACAGTGTCAATTTTAGAAAATAATAATTTACACATTTTTTTGA18.333333333333332No Hit
TCATGTATTCGGAATTACATTTCATCGACTAACGGGGTGAATCAATAATT18.333333333333332No Hit
ATTACAAAGAGAACCGCTTCATATAAATTGGAGTAACACTGACGGAAGAC18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)