FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGAAGAGCATCCCAGGGCTGGGAAGGCCCCTAAGAAAGCAGGAGGA11.0309278350515463No Hit
AAGTGGATGATGTCAATGAGATACCCAATTACAGCAGACAAGAGAATAAT11.0309278350515463No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT11.0309278350515463No Hit
GAAATAGCAATAAGACCCAAAGTGAGGGGTCAAGAAGGGAGAATGAACTA11.0309278350515463No Hit
ACCTATGATTGCCTTTGATCCCACAGCTTCTTAAACTCAAAACATATTCT11.0309278350515463No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGATTTCTTCAATGG11.0309278350515463No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC11.0309278350515463No Hit
CAAGATTCGCTGATACTGGATCTCCTCCATACAGCCATGGAACACTCACC11.0309278350515463No Hit
TCGCGAGGCTAGGCGTGTTGATTGAGTCCAGTATAGGTGCAATGCGCAGG11.0309278350515463No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT11.0309278350515463No Hit
AAACATAACACACCAATACTCAGAAAAGGTAAAGTGGACGACAACCACAG11.0309278350515463No Hit
CTTGTGTGTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAATGGGAA11.0309278350515463No Hit
GGAGAAGGTTTATTATTTTGACAGTGTCAAATGTCCAAATCACATCCCTC11.0309278350515463No Hit
ACCCAGGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTGAAGGAACTTAC11.0309278350515463No Hit
AACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGG11.0309278350515463No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC11.0309278350515463No Hit
GTTCCATCAAATCAGGGATGTGATTGGCAGATTTGACAATGACCAAATAA11.0309278350515463No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG11.0309278350515463No Hit
ACCAACACTACGTCGCCTTGCCCAATTAGCACATTAGCCTTCTCTCCGTT11.0309278350515463No Hit
TCATCAGTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACT11.0309278350515463No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG11.0309278350515463No Hit
ATCCTCAACCATGATCCTCTCACATTCACAGTCAATGAGGAAATTTGCAT11.0309278350515463No Hit
GCTGTTTGAAATTTTCCTTTGAGGATATTGCATCCCGACCAGTGAGTACC11.0309278350515463No Hit
GCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGAACCCGA11.0309278350515463No Hit
GTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATTGAAGACCCGA11.0309278350515463No Hit
GTCGGGGGCGGCCGAGGGGTCGGCGGGATGCGGCCGGTTGGGGGGCGGGC11.0309278350515463No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGCTTCCTTTGGCATATCC11.0309278350515463No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC11.0309278350515463No Hit
ATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATATGTG11.0309278350515463No Hit
ATGTTACATTGCTGGCTGGATCATGGGAAATCCAGAGTGTGATTCACTCT11.0309278350515463No Hit
CACCCTCAACGTGCCCAGACAGCGCGGAATCCAGCGTAGACTATTTGTAC11.0309278350515463No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC11.0309278350515463No Hit
AGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTG11.0309278350515463No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT11.0309278350515463No Hit
ATGCAGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTTTCAAG11.0309278350515463No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG11.0309278350515463No Hit
CACACATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAATCATAAA11.0309278350515463No Hit
ACCTTGTTTTACGGACCAATTCTCTTTCTAGCATGTACGCCACCATCAAG11.0309278350515463No Hit
ACAACCCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGAT11.0309278350515463No Hit
CAATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATGCCGTTGT11.0309278350515463No Hit
ATGCACTCCCATACGATTCTGGTAGGCATGCAAAATTTAAATAAGCTCAT11.0309278350515463No Hit
CAGCAGAAGTGTCCCACAGCAGGGCTACTGAATACATAATGAAGGGAGTG11.0309278350515463No Hit
CCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTT11.0309278350515463No Hit
ATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAG11.0309278350515463No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAAATT11.0309278350515463No Hit
GATCTGAATGGCCAGTGTTTGTATCAACTCTCATCCTTATTTTAACTTTA11.0309278350515463No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACAATGGACACAGTAAA11.0309278350515463No Hit
AATCTGGAGATTCTACATCCAAATGTGCACTGAACTCAAACTCAGTGATT11.0309278350515463No Hit
GTGTTCTAGAGGCTATGGCTATCCTTGAAGAATCCCACCCAGGAATATAT11.0309278350515463No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC11.0309278350515463No Hit
GTTCACCACATCAGAATCATTTATCAAATGAGACCTTCAGATTAAAATGA11.0309278350515463No Hit
GCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAA11.0309278350515463No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT11.0309278350515463No Hit
GGGGGGGGGGGGGGGGGTGGGGGGGGGGTGGTGGGGGTGGGGGGGGGGGG11.0309278350515463No Hit
ACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAATAAA11.0309278350515463No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG11.0309278350515463No Hit
ATCCTATATGGCAAGGTCGACATGCAGCTTTGCCTTGCTGGACCCGTTGG11.0309278350515463No Hit
TCCCCAGCCCGTACTTAGCATGTAGAACATTCCCATCATCATCCCAGGAC11.0309278350515463No Hit
CGAGAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTTAAGA11.0309278350515463No Hit
GAATTGGTCCGTAAAAAAAGGTTTCTCCCAGTAGCCGTCGGAACATGCAG11.0309278350515463No Hit
GGATGTGATTATGGAAGTTGTTTTCGCAATTGAAGTGGGGGCAAGAATAC11.0309278350515463No Hit
GGTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCC11.0309278350515463No Hit
TCCCATGACTAATAGATGCTTTGCCATCTATTAGAAGAGGCCTGATTTTC11.0309278350515463No Hit
TAGGTGGATGGTGAATGCCCCATAGCACGAGGACTTCGTTCCCGTTATCA11.0309278350515463No Hit
GTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGG11.0309278350515463No Hit
ATATAGCCCCCCAAGATCGATGGTTACTGGTTCCACGTTGTAGCTTAGTT11.0309278350515463No Hit
GATCGCCCCTAACTGCCTTGATCATGCACTCCGCCGGTGAGATTACCATG11.0309278350515463No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG11.0309278350515463No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT11.0309278350515463No Hit
ATCGTCAACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCA11.0309278350515463No Hit
GTTGAACCAAGATGCATTAAGCAAACCCCAAGGATCATTAATCAGGCACT11.0309278350515463No Hit
CGTCGGGGGGGGTGCGGGGGGGGGGGCTCTGGGGGGGGGCGGGGTGGGGG11.0309278350515463No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG11.0309278350515463No Hit
ATCCAGTGCTGGTCTGAAAGATGAACTTCTTGAAAATTTTCAGGAATACA11.0309278350515463No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA11.0309278350515463No Hit
ATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTTCA11.0309278350515463No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGCCTCTTATCCCTCTCAG11.0309278350515463No Hit
GTATGATCATAATCGGGGAAGACGTTGTCATCCCCTGAATCCCTTTGGCA11.0309278350515463No Hit
CACTACCCCTATCTCCTTGCAGGAGTAAACATCTACACCCAATTTGCTGA11.0309278350515463No Hit
TTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGT11.0309278350515463No Hit
ACATGGTGGAATAGGAATGGCCCAACATCAAGTACACTTCAAAAACCTAA11.0309278350515463No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA11.0309278350515463No Hit
CTCTAGCTCTATCTTGACAAAATGACAATCGTAAACATCCACAGCACTCT11.0309278350515463No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA11.0309278350515463No Hit
ACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTC11.0309278350515463No Hit
GAATGAAAATCAAAATACTCGAATGTTCCTGGCGATGATTACATATATCA11.0309278350515463No Hit
CAATTTTAATGGCTAACTTAATATAACAAAATGGAAACATAATATCAATA11.0309278350515463No Hit
GATTCAGTGCAAGTCAACACTAATCAATCTATAATCAGGAACAGGGAAAT11.0309278350515463No Hit
GAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAATGACAAATAACAT11.0309278350515463No Hit
TATGGATAGCAAATAGTAGCATTCCCACAACTACGTCAGTGCATGTGTGA11.0309278350515463No Hit
ATAATGAATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGGGA11.0309278350515463No Hit
CTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGAC11.0309278350515463No Hit
CTGTGCCACCAATGGGGCGGGGGCTGGAGTCGGCGGGGGGGCAACAAACC11.0309278350515463No Hit
ATGTAATGTAATAAATGAAAAAAGAGAAAATAGAGATAACAACCCACTTA11.0309278350515463No Hit
GTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAGCAGAT11.0309278350515463No Hit
GAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCACC11.0309278350515463No Hit
CTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCATGA11.0309278350515463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG50.0145.05
AGCCCGT50.0145.06
CCCAGCC50.0145.03
CCAGCCC50.0145.04
CATTTCT50.0145.0145
CCCGTAC50.0145.08
CCGTACT50.0145.09
GCCCGTA50.0145.07
CCCCAGC50.0145.02
TCCCCAG50.0145.01
Produced by FastQC (version 0.11.5)