FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R1_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGC13.7037037037037033No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG13.7037037037037033No Hit
ATGGACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACACAC13.7037037037037033No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC13.7037037037037033No Hit
GTATTTGGGGGCTTAAATACGCAGTAGTGTAATTACTGGAGCATACGGTG13.7037037037037033No Hit
CAAGTAGGCAGAGATGCAGGCAGAGAGACAGAGGAGGAAGCAGGCTCCCC13.7037037037037033No Hit
CTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCA13.7037037037037033No Hit
GAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCCATAATG13.7037037037037033No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC13.7037037037037033No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCTGTATGGTCCTGCACACTT13.7037037037037033No Hit
AATCATGGCCTGGACATTGGTGAACAGTATATGAACAAGAAAATCTATCA13.7037037037037033No Hit
CTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAAT13.7037037037037033No Hit
GGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACA13.7037037037037033No Hit
CCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGT13.7037037037037033No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.7037037037037033No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13.7037037037037033No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA13.7037037037037033No Hit
GCCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACT13.7037037037037033No Hit
GTCATACTCCTCTGCAATGTCTCAGAAGAAATAAGACCCTTCATTACTAA13.7037037037037033No Hit
GCCTACCCTAACATGAAAGGTTATGCTAGGGCAAGGTTATGCTAGGGTAG13.7037037037037033No Hit
ATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTCCT13.7037037037037033No Hit
CTTTCCCAATAGAGCCTTCCTACACTCCCCTGGGTGCCTCTCCGATTGGC13.7037037037037033No Hit
CATTTGAGTTGAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTA13.7037037037037033No Hit
GCTAGCACCTGCTTCCAATCCATGAGGTAATTGGGATATATGCCTTTCTC13.7037037037037033No Hit
CATCTTAAGTAGATTCCTCTTGACTTGCAAAGAGGAATTTCAATAGAGGC13.7037037037037033No Hit
CCCCTGAAATGGTCTAGCACTTTTGCATCTTTTTGGAAATCCCTCAAGAG13.7037037037037033No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)