FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAACACTGATAAATGATTAAATAATATTGAAAAAGGAAGATTATGAG12.857142857142857No Hit
GTATGCTAGAGTCCCGGTTAAGTTTAATTACCAAAACCACGACGACTTGC12.857142857142857No Hit
AATTTATTTTCATTGAAATAGTGAGGTGGAATTTTTTGAGTCTCTCACTC12.857142857142857No Hit
ATCCCGTATTGACGCCGCGCAAGAGCAACTCGGGAGCCGAATACACTATT12.857142857142857No Hit
GTCCTTCCGAGTGAGAGACACAAAAAATTACAACACACTATTGAAAAGAA12.857142857142857No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC12.857142857142857No Hit
GGCACAGGAGGTAATCAGGGAAGTTGTTTTCCATAACGAAGTGGGATACA12.857142857142857No Hit
CTTAAGAACAGCACGACAGCTACCAAAAGAATTCGGATTGCCATCAATTA12.857142857142857No Hit
TTATTATCCCGTATTTAAGCCGGGCAAGAGCAACTCGGTCGCCGCATACA12.857142857142857No Hit
CTCTTACTGTCATGCCAACCGTAAGATGCTTTTCTGTGACTGGTGAGTAC12.857142857142857No Hit
CTAAGGGATTAGAACGCTTTTCAACGAAGGGTCCGGTAGTAAAAGGACAA12.857142857142857No Hit
CCCACTGGCTGGTGGAACAAGCAGTGTGTACATTCAAGAGTTGCATTTGA12.857142857142857No Hit
CCGGGTATGTGTGCCAGGCTGCACTGGGACTGAGCATGAGCGCCTCCGTC12.857142857142857No Hit
CCCTGGACCATACCACGGTCTCACAACTGTTCCTAAAATCACTATTAACG12.857142857142857No Hit
GTCCTGCAACTTTATCCGACTCCATCCAGTCTAGTAATTGTTGCCGGGAA12.857142857142857No Hit
AAACTACATATGTGCCTTAATCTGGGTCTTAAGTAAAAGGGCCAGCAACC12.857142857142857No Hit
CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCAC12.857142857142857No Hit
GGACTATGGTTGCTGACTAAGTGAGATGCGGAGCCGCTGGCACGACAGGT12.857142857142857No Hit
GTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTACAAACCCACT12.857142857142857No Hit
TCGCTCTGCTAATCCTGTTACCAGTGGCTTCTGCCAGTTTCGATAAGTCG12.857142857142857No Hit
TTTAACGATAATGAATTGTATTTGTTATTGGTACATTCCTATTCCAAAAT12.857142857142857No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTC12.857142857142857No Hit
CCCGAGGAGGTCCGTGAAACACAGGGAACAGAGAAACTGACAATAACTTA12.857142857142857No Hit
GTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGC12.857142857142857No Hit
TGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGA12.857142857142857No Hit
GTTATCACTCAAGAATATGGCAGCAATGCATAATTAACTAACACACATGA12.857142857142857No Hit
CTTATTACCTATTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACACAGA12.857142857142857No Hit
AACGGCTTCAGGACCATGCGTCTCCAACATCATTGACGATGAGTTAGTTC12.857142857142857No Hit
GATGTGTCCTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG12.857142857142857No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC12.857142857142857No Hit
TTCTTATGCTCGCTGTCTGGCTGTCACTAAGTATGCTAGAGTCACGTTAC12.857142857142857No Hit
GTTCTGCCTAAGGTTGTCTACCATCCTAATTCCACCAATCTGTGTGCTGT12.857142857142857No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG12.857142857142857No Hit
AGCTTATAGTGAGTGTGATAGAAGCAAAGTATATTGCCGAATCTATAATT12.857142857142857No Hit
CCCATTTTGCTGATTCTCACTCATCTAATTGACATCTCGATGCTTGGAGT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)