FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACAATCCTTACTCCTCCAATCTGTGTGATGTGGCACATTTCCAAGA11.3157894736842104No Hit
TTGAAGACCCGAGTCACGAGGGGGAGGGAATACAACTATATGAAGCAATC11.3157894736842104No Hit
GCAAGGGGGCTGAGATCTTGTCTGGGTTCATGAAACTGGTGAGGGCTGCC11.3157894736842104No Hit
CTTGTAAGGCTTCCATAAATCTTAGTTGTTATAAACTATTATCTGACGCT11.3157894736842104No Hit
CTACTGGGAGAAACCTTGGTTTACGGACCAATTCTCTTTATAGCATGTAC11.3157894736842104No Hit
GATTGGATAATTTCAAGATTCCTCTTGGTAATACAGGAATGTGTAGTTTC11.3157894736842104No Hit
AGTCTTACCAGATAATAATAATCCATTAGCTCCCAATTTAAGCAGACTTC11.3157894736842104No Hit
TCTCCCACATCATTGATGACGAATAAGTTATTGTCAAGTTCTCAGTTCCT11.3157894736842104No Hit
GTGATAAAGAACAACATGATAAACAAGGCCCTTGGACCTGCAACGGCCCA11.3157894736842104No Hit
GTGTCAAACGTCCCAAGCACATCCCGAATTTGATGGAACAGTGTCATTAC11.3157894736842104No Hit
GTCCCAAAGGAGAAAACCAAGTGATGCCTTCCACATCTGTGGAAGCGTTC11.3157894736842104No Hit
ATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAA11.3157894736842104No Hit
TTATCCAACTATTGCTCACTCCTCAGTGAAAGCCCTTAGGAGTATCACGG11.3157894736842104No Hit
CCACTGTACCGGATTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTC11.3157894736842104No Hit
GATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTC11.3157894736842104No Hit
GGTATGCACAAAAAGAATGTGTTCTAGAGGCTATGGATTTACTTGAAGAA11.3157894736842104No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATAAGTAAAGACTCGA11.3157894736842104No Hit
GGGTGACTCGCCGAATGGCCAAGTTTCCGATTTGTATTCAAAGATTTCAT11.3157894736842104No Hit
GCATATGTCTGCGGCTTGATCCTCAGTTGGATACTGTCTAAGGATGTCAA11.3157894736842104No Hit
AATACTCACAAACCCTATATCCGTGCTGGAGTATTCATCTACTACCATTT11.3157894736842104No Hit
TCTGGTACCTGTTTCTAAGTCTCACCAAGAAAACAATCATGCTGTCGCGG11.3157894736842104No Hit
ATGCTTCGCTGCGGGGGATCGGCTGGGGCGAGGGTGACCGCGGGTGAGCC11.3157894736842104No Hit
TTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTT11.3157894736842104No Hit
GGCCTCCACCATGCTTGAAATTCAAACCGGTCTGCTATATGTACTGCTAG11.3157894736842104No Hit
CATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAAT11.3157894736842104No Hit
GGCCAGGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATTATTACTCCC11.3157894736842104No Hit
ATGTTAAATAGCTTGCCAGAGATCCCCTTTTTTCCTGCTGTGTGAAAGTT11.3157894736842104No Hit
CATTAAATAAGATCCCCAAAGATGCAGAGAGATGCAACTTAAAAAGAAGG11.3157894736842104No Hit
CCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAAT11.3157894736842104No Hit
AAGGAGTACGATTTGATTCTGGAAATTATTCCTCCCGCCGACCACGTGGC11.3157894736842104No Hit
AATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAA11.3157894736842104No Hit
CATTAGTTGTAAGTCTTGCATAAATGTTATTTGTTAGAAACTATTCTATG11.3157894736842104No Hit
GGCCACGAGGGTACGGGATCACAGAGCCTGTGTGTCTTGCTCTGGCTCAG11.3157894736842104No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA11.3157894736842104No Hit
GGCTCTTTTGGGAAAGTGAGCAGGACCTTACTGGCAAAATCTGTATTCAA11.3157894736842104No Hit
GAAGTGGATTATTGAAATGAGATACACAATTACAGCAGAAAAGAGAATAA11.3157894736842104No Hit
GGTCAAAGTTCCTGCTGATGGATGCTCACAAAATAAGTATTGAAGACCCG11.3157894736842104No Hit
CTGCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATT11.3157894736842104No Hit
GTATGGAAGTGCTGCGGCACTCCTGAAACTAACATTACACTTTATGTGAA11.3157894736842104No Hit
GTGAGAGCTCTCAGCAGTCCATCGGGTTTGTAAGCGGAGTGCTCCCCCAG11.3157894736842104No Hit
GTGTGGATGGCATGCCACTCTGCTGCATTTGAAGATTTAAGAGTAGCAAG11.3157894736842104No Hit
CTCCCATTCTCAAAAAATTTTCTCCAAGAGAATATCTGTATATTTTAATA11.3157894736842104No Hit
GGCTTCTGGTTGCCTTAGTGACAAGAGACTGAAATGGTTCAAATTAAATT11.3157894736842104No Hit
GTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGC11.3157894736842104No Hit
ACCTTCAACAAGCAGAGCTAGAGTCGGTTTGCAGGTCTATCCTAATACTC11.3157894736842104No Hit
CCTGTAGCTCTGCTAGCCCCTGCTTCCCAGCCATGAGGTAATTGGGATTT11.3157894736842104No Hit
CACCGCTAAGAATCATCCAGTAGCTCACATTTTAAGAAGCCTTCATGAAT11.3157894736842104No Hit
ACAATACTGCCAATTGACCAATTAGCACAGTATCATGCTCTACTGGTGCA11.3157894736842104No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA11.3157894736842104No Hit
TGGTAGCGCAGAATCATACGGGAGAAATTTTATTATTTGGACAGTTTCAA11.3157894736842104No Hit
ATAATGCCCCCATACAGGTTTTTTTTCCGTCGTCCTTCTATGGTCCTACA11.3157894736842104No Hit
GCATAAAGCCAAGTAACAAATAGCTACCTTCCACAATCAGAGCTAGACTC11.3157894736842104No Hit
CAATGGGAGAGTGTGAGCGGATGCAGTCGGGCCCTGGGGAGCAGGGCTGC11.3157894736842104No Hit
GAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCC11.3157894736842104No Hit
CACACACACCCAATTTATTGAGGAGTTTGCAGTGGTGTTTTTTGGTTTGT11.3157894736842104No Hit
TCTTAAAGTAACAGAAAAGTGGCTACAAACCCACTTAGAGCACCAAACAG11.3157894736842104No Hit
CAAAAATAAGTCACATCATCACTGACACCTGGGAAGCTGCTGGCCATATA11.3157894736842104No Hit
GTCCCTGTGCTGTGGGTCCCTGTGCTGGTGGCCTGACCGTGCCGCCTCTC11.3157894736842104No Hit
GATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAACGTCTCCAACATA11.3157894736842104No Hit
ATACATTGATAAGTGTTGACTAGCACAGACTCAGCGCCATTGAACTCCCA11.3157894736842104No Hit
ATGCAGTGTGATCCGATTTGATCCTTACCTATCCTCAGCTGGATTTCTGT11.3157894736842104No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCTAAAAGGTCGAAAGGTAGA11.3157894736842104No Hit
GATGTTGGAGACCTTAAACAGTATGACATTGATGCTCCAGAGCCCAGCTC11.3157894736842104No Hit
GGACAGGGCCGATGGTCCCATGTTTCAACCTTTCGACCTTTTCGAAATAA11.3157894736842104No Hit
GGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAG11.3157894736842104No Hit
CATTTGAATCGTGTTAGTCAAAAGTTCTTTCATTTTAAAATATAACCAAA11.3157894736842104No Hit
CACCAGTTTCATGAACCCAGACAAGATCTCAGCCCCCTTGCTCATGCCCC11.3157894736842104No Hit
GTCTAAGGATGTCCACCATCCTTACGCATCCAATCTGTGTGCTGTGGCAC11.3157894736842104No Hit
GCTGTGGCCCACCCGCCACACAGCATTTCCTCGTCAGGAGCTGCCAGGGT11.3157894736842104No Hit
GAATAAGATTGGTCCTCCATAATAAAATAATAGAAGTACCTTTGATAGAG11.3157894736842104No Hit
GGCCCGGGGGTACCCCTGGGTGGACAGCGGGGGAGCCCGGGCTCAGCTCA11.3157894736842104No Hit
GTGTATGCACGCGTCTCTGCACGTGCCTCGGTTGTCGCTATAGGACATGA11.3157894736842104No Hit
CCCTGGATCATGACTACATAAGACGCTAACACTTACACATCAGTCCAAAC11.3157894736842104No Hit
ATGTTAAAGGATTGATTCCACCTACTTAAGAATTCGCTCATATGGCAATT11.3157894736842104No Hit
AGTGAATTGTTGACCGGAACCACCTTTACGTGCTGGTATAATATGTACAC11.3157894736842104No Hit
AAGCAGGGCAGAGGAGTCGATAGCTGGATGGGAAGGCAAGGGAGAAGGGA11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAA50.0145.01
TACAGGT50.0145.08
ATCTAAT50.0145.02
GAAAATA50.0145.0145
TCTAATA50.0145.03
CTAATAC50.0145.04
TAATACA50.0145.05
AATACAG50.0145.06
ACAGGTC50.0145.09
ATACAGG50.0145.07
Produced by FastQC (version 0.11.5)