FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.36596523330283626No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG70.32021957913998167No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.2744739249771272No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG60.2744739249771272No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA50.22872827081427266No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC50.22872827081427266No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG50.22872827081427266No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC50.22872827081427266No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG50.22872827081427266No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC50.22872827081427266No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC50.22872827081427266No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC40.18298261665141813No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA40.18298261665141813No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG40.18298261665141813No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT40.18298261665141813No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC40.18298261665141813No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.18298261665141813No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA40.18298261665141813No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT40.18298261665141813No Hit
ATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTC40.18298261665141813No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA40.18298261665141813No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.18298261665141813No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC40.18298261665141813No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG40.18298261665141813No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.18298261665141813No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.18298261665141813No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.18298261665141813No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT40.18298261665141813No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.18298261665141813No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.18298261665141813No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT40.18298261665141813No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA40.18298261665141813No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA40.18298261665141813No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.1372369624885636No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30.1372369624885636No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.1372369624885636No Hit
GGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTA30.1372369624885636No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.1372369624885636No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA30.1372369624885636No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA30.1372369624885636No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC30.1372369624885636No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30.1372369624885636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1372369624885636No Hit
ACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGGTAGCT30.1372369624885636No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA30.1372369624885636No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT30.1372369624885636No Hit
ACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACT30.1372369624885636No Hit
CCCCACTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG30.1372369624885636No Hit
ATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCA30.1372369624885636No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT30.1372369624885636No Hit
GTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGAC30.1372369624885636No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC30.1372369624885636No Hit
TCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCC30.1372369624885636No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT30.1372369624885636No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG30.1372369624885636No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT30.1372369624885636No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA30.1372369624885636No Hit
CTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA30.1372369624885636No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30.1372369624885636No Hit
CATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCA30.1372369624885636No Hit
TCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACA30.1372369624885636No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT30.1372369624885636No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC30.1372369624885636No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.1372369624885636No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.1372369624885636No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.1372369624885636No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT30.1372369624885636No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA30.1372369624885636No Hit
ATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGATTCAAT30.1372369624885636No Hit
CAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTT30.1372369624885636No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.1372369624885636No Hit
CTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGA30.1372369624885636No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG30.1372369624885636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCATG100.0066095586145.07
Produced by FastQC (version 0.11.5)