FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAAGCTTACATGTTCGAGTCTAAAAGAAAGAAGATTCGAACACAAAT15.555555555555555No Hit
GTCTATTACTCGGTCGATCCTATCCGTCAAGTGAGTCTGACATCCTGGGG15.555555555555555No Hit
GAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCC15.555555555555555No Hit
GTCACGGTAGTCGTGAGCTATGACGGATTTTTGAGAATCGGGCTGCACCC15.555555555555555No Hit
GCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTT15.555555555555555No Hit
GTTCATTGATGCTTAAGGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA15.555555555555555No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG15.555555555555555No Hit
CATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAAT15.555555555555555No Hit
GCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGT15.555555555555555No Hit
GACTGAATTAGCGATGCTATCTAATAAGAGGACTGGGAGTTAATACCACG15.555555555555555No Hit
CTCGTGGTCAGGCTGTGTGGTTTCTGCCGTAGTGTTCGTTGCGGGCCCAT15.555555555555555No Hit
TTCTTGAGGACGNCANCNNGCCNNNNNNNNNTNNNNNNNNNNNNNNNNNN15.555555555555555No Hit
CCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCC15.555555555555555No Hit
GGACATGGCCACCTAAGGAGTGTTGACCGATGAGTTAGTCAACTGGGGAA15.555555555555555No Hit
CGTACACACCATGGCCAGCTCCAAGAAAGAAAAACCAACCGGTCTCCAAT15.555555555555555No Hit
ACAATGAGGAAAGTGCGCTAACACCCCACTGTCAAAGAAAAAGGAGAGTA15.555555555555555No Hit
CTAAAATACATCCCGTCATGGTCCCGTACATGCTAGAAAGAGAATTGTTC15.555555555555555No Hit
AGGTAAGAAAATATAGAAAGTTTAAAAGAAATGTAACATCGTAACTTGAG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)