FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAAGCGTGCAGGCGGGCTATGCAGAGTTCACCATGGGGGGTCGCAGC15.263157894736842No Hit
GTTGTTTTCCCTAACGAAGTGGGAGCCAGGATACTAACATCGGAATCGCA15.263157894736842No Hit
AGGAACATAGTGAGAAGATCTGCAGTATCAGCAGATACACTAGCATATTT15.263157894736842No Hit
AATTGACATCTCGATGATTTGAGTCATGTATGGCAATATCCCAATCATTA15.263157894736842No Hit
GTAATAGGAATCGACCAATAACAAATACAGATCAATATCCAAAAATCAAC15.263157894736842No Hit
CGCGAGCTCAGCTCGGGGTTCAGATCAAAGGTCTGAAGGGCTGGTTGCAA15.263157894736842No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG15.263157894736842No Hit
GTATCTGGGTGTGTCGGTGTGTCTGCCTGTCACTCTGTCTCTCTATTTTG15.263157894736842No Hit
GTAATAACTCTGTTACAACAAATGAGGGATTTGCTTGGGAAATTTGATAC15.263157894736842No Hit
GCTGTATACTCCAGGAGGGGAATTGAGGAATGATGATGTTGATCAAAGCT15.263157894736842No Hit
AGTAGAAACAAGGTAGTTTTTAAACTATTCGAAACTAATTGATGGCCCAC15.263157894736842No Hit
ACATAAAACCAGGTCATGCCGCGCTCAGAGCGAACTAGGCACAGGAAGTA15.263157894736842No Hit
ATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAATGT15.263157894736842No Hit
CACCAGAATCGGACGTGAGGCTAATGAGAAGAGGGTAAATATCCCCCACA15.263157894736842No Hit
GTTATAAAGAACAACATGATAAACAATGCCCTAGGACCTGCAACGGCCCA15.263157894736842No Hit
CCGAAAACAAGGAAACGAATAGACGCCAAATTCTATTAAAGTAGGCTGTA15.263157894736842No Hit
GTCTGGCTGTCAGTAATTATGATAGATTAAAGTTTACGTTTCATTAAAAC15.263157894736842No Hit
ATACAGGAGAATGGCCCCTTACAAGTATTCTCATTACTGATCACCTAACA15.263157894736842No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATATTCACAAGGATGCGAAAG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)